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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 61 for “"gel filtration chromatography"”.
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A study of the aggregating cationic antibacterial proteins and peptides in bovine seminal plasma
… and 20 kDa were identified and isolated using gel filtration chromatography at neutral pH. Acidic gel filtration chromatography, cation exchange chromatography using citrate buffer and anion exchange chromatography at pH 12.0 and pH 13.0 were found to be successful techniques for disaggregation …
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Molecular properties of aspartate transcarbamoylase and related enaymes from wheat
… enzymes has been investigated in wheat using gel filtration chromatography. CPSase activity could not be detected in gel filtered extracts and in crude extracts from wheat seedlings was shown to be highly labile. ATCase and DHOase activity was detected and the molecular weights of these …
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The Isolation and Evaluation of Biological Effects of the Glucosinolate Hydrolysis Product 1-Isothiocyanato-3-(methylsulfinyl)-Propane
… by hydrolysis product purification using gel filtration chromatography and reversed-phase HPLC.
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Mechanisms of Immunosuppression in Murine Malaria
… the suppressor factor was possible by means of gel filtration chromatography on Sephadex G-150 using sodium borate buffer containing 6M guanidine hydrochloride. The purified soluble suppressive factor was tentatively characterized as a small glycoprotein (M.W. 27,000 daltons), possessing …
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Suspended microchannel resonators for ultralow volume universal detection
… the potential for real-time monitoring, gel filtration chromatography was used to separate different molecular weights of PEG as the SMR acquired a chromatogram by measuring the eluate density. This work suggests that the SMR could offer a simple and sensitive universal detector for …
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Isolation and Characterization of Blood Lipoproteins From Dairy Cows Fed Control or High Grain Diets (Lactation, Feeding, Lipids)
… using either preparative ultracentrifugation or gel filtration chromatography. Lipoprotein classes obtained by ultracentrifugation were very low density plus chylomicrons, low density, high density(,1), and high density. These lipoprotein classes were applied individually to an agarose gel column …
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Studies of a low molecular weight Zn-containing protein population of lens tissue
… the total lens Zn, 25% was recovered in the ultrafiltration fraction of less than 20,000 molecular weight which contained 1% of the total soluble protein. Lens tissue has low molecular weight TCA-soluble components that can bind Hg. Isoelectric points of low molecular weight protein fractions were …
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Characterizing the oligomeric structure and catalytic activity of the dihydroorotase and aspartate transcarbamoylase from the bacterium, <i>bacillus anthracis</i>
… The proteins were purified by affinity chromatography and the enzymatic activity was determined by enzyme assays. The data suggests that a physical and functional interaction exists. The activity of ATCase was increased by about 2 fold in the presence of an equimolar concentration of …
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Purification, characterization and substrate specificity of a nuclear mRNA (Guanine-7)-Methyltransferase from Ehrlich ascites cells
… molecular weight of 95,000, as determined by gel filtration chromatography. SDS-PAGE showed a predominant protein band of 46,000 daltons suggesting that the native enzyme consisted of two identical subunits. The enzyme was unstable when the protein concentration was low but was stable when …
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Isolation and partial characterization of a water stress protein of the desiccation-tolerant cyanobacterium Nostoc commune UTEX 584 expressed in Escherichia coli
… was then applied to a Superose 12 HR 10/30 gel filtration column. The eluent fraction containing the ORF 24 product was then reapplied to the Superose 12 to yield the final fraction containing only the ORF 24 product. The final fraction of ORF 24 was purified to homogeneity as determined by …
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Transient collagen triple-helix binding to membrane type 1 matrix metalloproteinase : interaction studies and NMR-guided structural docking
… and hemopexin domains. The results from gel-filtration chromatography, DLS and NMR combined suggested that MMP-14 hemopexin domain behaves consistently as a monomer in solution. NMR-monitored THP titration led to identification of a distinct patch centered about blade I at the exit side …
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The functions of pregnancy-associated plasma protein A in rheumatoid arthritis.
… plasma using ammonium sulphate precipitation, gel filtration chromatography, ion exchange chromatography and affinity chromatography. The purity has been confirmed by SDS PAGE and western blot analysis. The purified PAPP-A has been used to investigate functions of PAPP-A which may support or …
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The Substrate and Regulator of Acetyltransferase San
… is still under way, and preliminary data from gel filtration chromatography suggest that the inhibitor may be a multi-subunit protein complex.
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Metal binding to transferrin and immune reactions in Parkinson's disease
… protein transferrin (Tf) was investigated by G75 gel filtration chromatography in control patients and treated and untreated patients with Parkinson's disease (PD). Fe-Tf binding was 100% in all controls and PD patients suggesting that a defect in Fe-Tf binding was not involved in the aetiology of …
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Isolation and Immunochemical Characterization of Soluble Anaplasma Marginale Antigen (Rickettsia, Hemoparasite, Disease, Elisa)
… antigen from infected bovine erythrocytes. Gel filtration in Sephadex G-200 and anion--exchange chromatography in DEAE cellulose were used to isolate this antigen from infected erythrocytes. A fraction with antigenic activity was eluted in the ascending phase of the hemoglobin peak by gel …
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Purification and Characterization of the Cytochrome BC(1) Complex From Rhodobacter Sphaeroides
… glycerol, and was purified by anion exchange and gel filtration chromatography. SDS-polyacrylamide gel electrophoresis showed the presence of four polypeptide subunits with apparent molecular weight values of 40, 34, 27, and 14 kDa. Values for ubiquinol, phospholipid, and iron content were …
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In vitro proteolysis of ovine lens crystallins by calpain II : A dissertation submitted in partial fulfilment of the requirements for the Degree of Bachelor of Science with Honours
… ammonium sulphate precipitation, hydrophobic chromatography and ion exchange chromatography, while the crystallins were purified using ion exchange and gel filtration chromatography. Calpain II was incubated separately with α-, β-and γ- crystallin and the resulting products were analysed by …
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The effects of thymic hormones on the proliferation of human myelogenous leukemia cells
… cytostasis. Further purification of TF5 by gel filtration chromatography produces a biologically active factor of approximately 20 kDa. Since the parent compound is composed of peptides less than 15 kDa, these results suggest the possible formation of oligomers. Electrophoresis of the active …
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The growth and stress response characterization of Synechococcus WH8109 cyanobacteria
… homologue of GroEL using anion exchange and gel filtration chromatography and sucrose gradient centrifugation. The expression levels of this chaperone were analyzed under normal and stress conditions and they have been shown to respond to heat shock and infection.
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Purification and characterization of a protein phosphatase (PP1-Arch) from the archaebacterium Sulfolobus solfataricus, isolation and expression of its gene
… 1OOO-fold by DE-52 ion-exchange, hydroxyapatite, gel filtration (G- 100), and Mono Q FPLC chromatography. PP1-Arch was identified from the final purified sample by renaturation on an SDS-polyacrylamide gel. The molecular size of PP1-Arch was determined by both gel filtration chromatography and …
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