Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"gastruloids"”.
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WNT signalling in the patterning of human gastruloids
… of WNT signalling in the patterning of human gastruloids. By applying different concentrations of WNT agonists, it is shown that the levels of WNT signalling affect cell differentiation as well as elongation and morphology of the gastruloids. Higher WNT levels induce Brachyury expression, …
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Investigating Wnt3-mediated symmetry breaking and cell fate in engineered gastruloids
… aggregates of embryonic stem cells (ESCs) termed gastruloids are able to display symmetry breaking and develop an AP axis in the absence of extraembryonic tissues or localised exogenous positional signalling (Baillie-Johnson, van den Brink, Balayo, Turner, & Arias, 2015; Beccari et al., 2018; …
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Towards using organoids in functional genomics – A single cell characterisation of mouse gastruloids
… an organoid model of mouse gastrulation called gastruloids, as a model for such perturbation studies. Gastruloids are grown by aggregating around 300 mESCs for 48hrs, followed by a 24-hr treatment with a WNT agonist which stimulates the gastruloids to produce all three main germ lineages. …
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Developing a stem-cell system of embryonic development organised by a morphogen signaling centre
… stem cell derived embryo-like structures called gastruloids are able to circumvent these impediments posed by in vivo research. Gastruloids form derivatives of all three germ layers in a spatially coordinated manner and resemble key in vivo aspects of gastrulation. In addition, gastruloids are …
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The Generation of a Candidate Axial Precursor in Three Dimensional Aggregates of Mouse Embryonic Stem Cells
… of small, three-dimensional aggregates of ESCs (gastruloids) that can recreate the events of early post-implantation development, including axial elongation. Gastruloids are the first ESC-based model for axial elongation morphogenesis; this body of work characterises their development and …
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The effects of BMP4 on different in vitro models for mouse gastrulation and neural induction
… is required for neural induction to take place. Gastruloids are a 3D culture model that does not require BMP4 to undergo gastrulation-like events. This thesis describes the effects of BMP4 on gastruloids and on a 2D adherent cell culture model for gastrulation, demonstrating the interactions …
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Modelling Embryonic Haematopoiesis Using In Vitro State-of-art Gastruloid Culture
… the culture. Under the new culture conditions, gastruloids can generate haemogenic endothelium and pro- and pre-HSCs in sequential order, as detected by a combination of markers in flow cytometry assays (Flk-1, c-Kit, CD41 and CD45). Concomitant use of cytokines VEGF and FGF2 can facilitate the …
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Pluripotency and patterning in nonhuman primate embryogenesis
… streak and amnion formation in micropatterned gastruloids, similar to human. WNT was critically essential for initiation of the primitive streak, while Hedgehog did not exhibit patterning effects. To explore the transcriptional circuitry of anterior-posterior patterning, I screened 1000 …
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Cell Memory and Fate in Human Development
… a stem cell-based model of gastrulation (human gastruloids) to disentangle the function and timing of two key signaling pathways, namely Wnt and Activin/Nodal. From studies in model organisms both pathways are known to be required for initiating gastrulation and for the formation of mesoderm and …
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The Role and Control of WNT Signalling in an HESC Model of Human Primitive Streak
… transplantation strategy grafting treated gastruloids into chick embryos. The assay demonstrated that the human cells induce and contribute autonomously to a secondary axis while inducing neural fate in the host, thus fulfilling the most stringent criteria for an organizer. This work adds …
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A Translational Approach to Modeling Unique Aspects of Germ Cell Development During Self-Organization of the Primate Embryo
<p>The specification of germ cells during embryonic development is vital not only for the development of an organism, but quite literally for the survival and propagation of its entire species. Recent work has demonstrated that several aspects of human primordial germ cell (hPGC) development are …
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Probing early human embryonic development to characterize BMP signaling stimulators
Bone morphogenetic proteins (BMPs) sind sowohl in der Embryonalentwicklung als auch im adulten Organismus von entscheidender Bedeutung. Da ein fehlregulierter Signalweg mit zahlreichen chronischen und schwerwiegenden Krankheiten assoziiert ist, stellt dessen Modulierung einen vielversprechenden …