Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"gastruloid"”.
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Modelling Embryonic Haematopoiesis Using In Vitro State-of-art Gastruloid Culture
… elusive. A novel 3D culture method, known as a gastruloid, can mimic gastrulation in embryogenesis, which is crucial to the emergence of HSPC precursors, highlighting the potential to develop this protocol as an in vitro platform for haematopoietic research. This project aims to adapt the …
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WNT signalling in the patterning of human gastruloids
… early development. One of these models is the ‘gastruloid’ which results from the aggregation of small numbers of hESCs and leads to the formation of the body plan. WNT signalling, an evolutionary conserved pathway, is known to play a crucial role during gastrulation with its dysregulation …
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Developing a stem-cell system of embryonic development organised by a morphogen signaling centre
… stem cell derived embryo-like structures called gastruloids are able to circumvent these impediments posed by in vivo research. Gastruloids form derivatives of all three germ layers in a spatially coordinated manner and resemble key in vivo aspects of gastrulation. In addition, gastruloids are …
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A Translational Approach to Modeling Unique Aspects of Germ Cell Development During Self-Organization of the Primate Embryo
… cell model of a gastrulating embryo known as a gastruloid. An hPGC transcriptomic signature that indicates migratory potential via canonical and novel mechanisms is indicated, raising several potential candidates for further investigation into the under-studied migratory phase of germ cell …
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The Role and Control of WNT Signalling in an HESC Model of Human Primitive Streak
… human embryonic stem cell (hESC) micropatterned "gastruloid" system. I first showed that in the human PS there is a conserved BMP → WNT→ NODAL signalling initiation hierarchy, and that WNT is necessary and sufficient for PS formation. Next, I found that structured subpopulations of endoderm and …
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The effects of BMP4 on different in vitro models for mouse gastrulation and neural induction
… is required for neural induction to take place. Gastruloids are a 3D culture model that does not require BMP4 to undergo gastrulation-like events. This thesis describes the effects of BMP4 on gastruloids and on a 2D adherent cell culture model for gastrulation, demonstrating the interactions …
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The Generation of a Candidate Axial Precursor in Three Dimensional Aggregates of Mouse Embryonic Stem Cells
… of small, three-dimensional aggregates of ESCs (gastruloids) that can recreate the events of early post-implantation development, including axial elongation. Gastruloids are the first ESC-based model for axial elongation morphogenesis; this body of work characterises their development and …
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Investigating Wnt3-mediated symmetry breaking and cell fate in engineered gastruloids
… aggregates of embryonic stem cells (ESCs) termed gastruloids are able to display symmetry breaking and develop an AP axis in the absence of extraembryonic tissues or localised exogenous positional signalling (Baillie-Johnson, van den Brink, Balayo, Turner, & Arias, 2015; Beccari et al., 2018; …
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A multi-lineage in vitro mouse embryo model derived exclusively from mouse embryonic stem cells
… only mouse embryonic stem cells (ESCs), the gastruloid model has been demonstrated to mimic basic body axis formation and aspects of gastrulation, somitogenesis, cardiogenesis, and neurulation. However, it fails to recapitulate the spatiotemporal interplay of signalling pathways between …
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Investigating the role of DPPA2 and DPPA4 in the Epigenetic Control of Lineage Programs in Human Embryonic Stem Cells
… and show axial patterning defects upon human gastruloid formation. Epigenomic profiling following the loss of DPPA2/4 revealed a marked depletion of H3K27me3 at a subset of DPPA2/4-target regions, predominantly near to poised chromatin regions. Regions losing H3K27me3 were typically highly …
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Towards using organoids in functional genomics – A single cell characterisation of mouse gastruloids
… an organoid model of mouse gastrulation called gastruloids, as a model for such perturbation studies. Gastruloids are grown by aggregating around 300 mESCs for 48hrs, followed by a 24-hr treatment with a WNT agonist which stimulates the gastruloids to produce all three main germ lineages. …