Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"ganciclovir"”.
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Analysis of cytomegalovirus UL97 drug resistance mutations in patients receiving Ganciclovir
… mutations, because of the widespread use of ganciclovir, have been widely reported in international literature, particularly in the post-transplant setting. However, a genotypic assay to detect CMV drug resistance is not available in South Africa and the prevalence of these mutations is …
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Ocular pharmacokinetics and efficacy of various amino acid and dipeptide prodrugs of ganciclovir
… (TFT), idoxuridine (IDU), and vidarabine, ganciclovir (GCV) gel and acyclovir (ACV) ointments. Some of the problems associated with thymidine analogues are their ocular and systemic toxicities in long term usage. Even though GCV and ACV have good efficacies against HSV, their limited …
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IDENTIFICATION OF HCMV UL97 IN GBM CELL LINES AND A POSSIBLE ROLE FOR GANCICLOVIR
… that inhibits DNA elongation and replication. Ganciclovir, a guanosine analogue, was used to treat GBM cell lines and our results demonstrate that it significantly decreases cellular proliferation in UL97 expressing cells. This project identifies a new role for HCMV in GBM and suggests a …
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Induzierter Zelltod von transdifferenzierten hepatischen Sternzellen (Myofibroblasten) auf der Basis des Thymidinkinase-Ganciclovir-Systems
In the current study the thymidine kinase / ganciclovir-system was used to develop a prophylaxis in oder to prevent liver fibrogenesis. The intention was to eliminate activated hepatic stellate cells (HSC), those cells which became proliferative, fibrogenetic and contractile because of liver …
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Viral HSV1-TK gene, radiolabeled FIAU, and ganciclovir: combined gene targeted radiotherapy and suicide gene therapy for prostate cancer
… kinase gene (HSV1-tk) transfer with the pro-drug ganciclovir (GCV) to be a promising suicide gene therapy in cancer. Unlike several other gene therapy systems, early-phase clinical trials of this strategy have shown encouraging results. Therefore, methods to improve its therapeutic efficacy are …
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Mechanisms of Cell Death Initiated in Herpes Simplex Virus Thymidine Kinase Expressing Colon Tumor Cells Treated with Ganciclovir and UCN-01
… Kinase (HSVtk) in combination with the prodrug, ganciclovir (GCV), has shown great promise for the genetic therapy of many cancers, but most patients have had only a partial or minimal response to the therapy. After screening a panel of two drug combinations, our laboratory has shown that the …
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Cytomegalovirus Infection in Heart and Lung Transplant Patients with focus on long-term outcome
… patients. CMV prevention with 14 weeks of oral ganciclovir reduced the incidence and severity and prolonged the time to onset of CMV disease, as compared with four weeks of intravenous ganciclovir in CMV seropositive patients. Our finding supports the hypothesis that a longer duration of CMV …
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Activation of Early Neural Progenitors Is Required for Traumatic Brain Injury-Induced Hippocampal Neurogenesis
… neural progenitors by exposing the animal to ganciclovir. Using this line, I demonstrate that ablation of dividing GFP-expressing early neural progenitors in neurogenic areas occurs only in the presence of ganciclovir. CCI on these mice, reveals that no newly born Dcx-expressing late neural …
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Cytomegalovirus retinitis at Groote Schuur Hospital : clinical management and outcomes
… Treatment consists of weekly intravitreal ganciclovir injections until clinical resolution has taken place. Patients not on highly active anti-retroviral treatment (HAART) are referred for initiation of treatment. Ganciclovir treatment is continued until immune reconstitution has taken …
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Reconstitution of a Multi-layered, Differentiated Cornea by HTERT-Immortalized Corneal Epithelial Cells Transduced with Thymidine Kinase Transplanted onto Denuded Mouse Corneas
… probes – PI, and Phalloidin. Cytotoxicity of ganciclovir was assessed at concentrations of 0.1, 0.5, 1.0, 5.0, and 10.0 μM using Live-Dead Assay. RESULTS: IF confirmed an intact corneal basement membrane following epithelial removal. At 4 hours and 1 day post-transplantation, CMFDA staining …
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Estudio de la incidencia de la infección por adenovirus, su cinética de replicación, y la respuesta inmune celular: impacto en la progresión clínica de pacientes adultos receptores de TPH
… tratamientos utilizados (cidofovir, ribavirina y ganciclovir) presentan una gran variabilidad en cuanto a su eficacia. El tratamiento de este tipo de infecciones debe basarse en el conocimiento de la cinética de replicación del virus, así como la identificación de la respuesta inmune específica …
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Tumor Response TCF-4/β-Catenin Regulatory Elements for Enhancing Cancer Gene Therapies
… kinase suicide gene in combination with ganciclovir was further evaluated in the normal and tumor cell lines. The Tcf-4/β-catenin elements conferred tumor specific expression of HSV thymidine kinase (HSV-TK) gene, resulting in selective metabolism of ganciclovir and cell killing of only …
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Expansion und retroviraler Gentransfer eines Suizidgens (HSV-TK) in humanen T-Lymphozyten zur adoptiven Immuntherapie
… 10% der in-vivo erreichbaren Gewebespiegeln) Ganciclovir zu einem Absterben aller Zellen nach 4 Tagen kam, während untransduzierte Zellen nicht in ihrem Proliferationsverhalten beeinflusst wurden. Nach Transduktion bleibt die T-lymphozytäre Funktion erhalten und eine spezifische …
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Molekulare Mechanismen des Tumorzelltodes induziert durch die Suizidgene Thymidinkinase und Purinnukleosidphosphorylase
… sein als das Herpes-simplex-Virus-Thymidinkinase/Ganciclovir (TK/GCV)-System. Um die molekularen Mechanismen des PNP/Fludarabin-induzierten Zelltods in humanen HCC-Zellen im Vergleich zu TK/GCV zu untersuchen, wurden humane HCC-Zellen der Zellinien HepG2 und Hep3B mit PNP oder TK über adenovirale …
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Simultaneous cytomegalovirus and herpesvirus meningitis in an adolescent immunocompetent patient a case report
… recibió terapia antiviral durante 21 días con ganciclovir (5 mg/kg/dosis), lo que condujo a un resultado favorable sin déficit neurológico posterior ni eventos adversos. Conclusiones: Este caso enriquece la práctica clínica al demostrar un manejo efectivo en una situación poco probable. …
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Targeting The Blood-Brain Barrier With A Non-Canonical Iron-Mimicry Mechanism
… genetic imaging and suicide gene delivery with ganciclovir. Finally, we expand our data by analyzing a large panel of primary CNS tumors through comprehensive tissue microarrays. Together, our approach and results provide a translational avenue for the detection and treatment of brain tumors.
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Gene Therapy for Osteosarcoma : "In Vitro" Characterization of Five Tumor Cell Lines and Development of Treatment Models "In Vivo"
… and the HS-tk gene effectively conferred ganciclovir (GCV) susceptibility to transduced cells. Each cell line showed a bystander effect (whereby wild type tumor cells are killed by co-incubation with HS-tk positive tumor cells and GCV). MNNG cells were used to develop a series of …
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Directed Migration Achieved in Mammalian Cells via Ca2+ Signal Rewiring in Synthetic Protein Chimeras
… engineered and TNFα source cells. Finally, post-ganciclovir treatment, cells undergo death via the thymidine kinase suicide mechanism. Hence, this forms the basis of engineering a cell to target inflammatory disease sites characterized by TNFα secretion and a low pH microenvironment. Lastly, we …
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EBV-Associated Gastric Cancer: From Initial Infection to Unique Therapeutic Approaches
… the use of HDAC inhibitors and the anti-viral ganciclovir for treatment of EBV+ epithelial tumors. Lastly, I have identified modulators of the response to a PI3Ka inhibitor in PIK3CA mutant gastric cancers. I found that loss of NEDD9 or inhibition of BCL-XL rendered cells hyper-sensitive to the …
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Topical Nanoparticulate Formulation of Drugs for Ocular Keratitis
… (NP) of dipeptide monoester prodrugs of ganciclovir (GCV) including L-Val-L-Val-GCV (LLGCV), L-Val-D-Val-GCV (LDGCV), D-Val-L-Val-GCV (DLGCV) were formulated and dispersed in thermosensitive PLGA-PEG-PLGA polymer gel for the treatment of herpes simplex virus type 1 (HSV-1) induced viral …
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