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Showing 1 to 20 of 182 for “"galactosidase"”.
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Towards novel luminescent probes for monitoring β- galactosidase activity
… in vivo the processing of sugars by β-galactosidases. To this end, a lactose-based probe was designed, featuring a Lanthanide held within a chelate and appended to the glucosyl unit, and a proximal naphthyl moiety, attached to the galactose unit, which would function as a sensitiser for …
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Alpha-galactosidase from Lactobacillus salivarius: Isolation, purification, and biochemical characterization
In this study the enzyme $\alpha$-galactosidase ($\alpha$-D-galactoside-galactohydrolase, EC 3.2.122) has been isolated and characterized from the soluble intracellular fraction of Lactobacillus salivarius. Growth on galactose, raffinose, melibiose and lactose produced the highest levels of …
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The Induced Synthesis and Properties of Beta-Galactosidase of Saccharomyces Fragilis
Made available in DSpace on 2014-12-05T21:45:28Z (GMT). No. of bitstreams: 1 0020863.pdf: 4404780 bytes, checksum: ede4ac7bc409c2f111529040f819bf08 (MD5) Previous issue date: 1957
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Synthesis of Bulky and Polar Galactonoamidines for the Inhibition of the Human α-Galactosidase
… potent competitive inhibitors of several β-galactosidases, with inhibition constants in the low nanomolar to picomolar range. The work described here summarizes an effort towards the synthesis of polar and bulky galactonoamidines for the inhibition of the human α-galactosidase. Following a …
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Investigating the effects of calreticulin and beta-galactosidase on microglial functions and neuronal loss
… loss were affected by calreticulin and β-galactosidase, two proteins associated with ageing, chronic inflammation, and neurodegeneration. The aggregation of amyloid-β to form oligomers and insoluble amyloid plaques in the brain is a hallmark of AD. Amyloid-β can be directly neurotoxic and …
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Development of a high throughput reporter system using β-Galactosidase in the yeast : Pichia Pastoris
… the lacZ reporter gene, which encodes for the β-galactosidase protein, and fused to the S. cerevisiae MATa signal sequence. Expression plasmids were successfully transformed in P. pastoris strain yGS 115 followed by induction. Western blot analyses confirm the expression of β-galactosidase and …
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Beta-Galactosidase: A Possible Model Enzyme for Studying the Genetics of Enzyme Regulation in Mammalian Systems
Made available in DSpace on 2014-12-14T15:38:02Z (GMT). No. of bitstreams: 1 7624126.pdf: 3286208 bytes, checksum: 0672cacc55267684c7bf8a3730256c70 (MD5) Previous issue date: 1976
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THE EFFECT OF STRINGENT CONTROL ON THE PRODUCTION OF ENTEROTOXIN, TRYPTOPHANASE, AND BETA-GALACTOSIDASE BY ESCHERICHIA COLI IN A RECYCLING FERMENTOR
… phases 2 and 3, while tryptophanase and (beta)-galactosidase were more negatively affected by ppGpp. An enhancing effect by glucose was found on the production of enterotoxin and (beta)-galactosidase. The nicking process of enterotoxin activation appears to be regulated by ppGpp and may be an …
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Isolation and Characterization of Thermostable Alpha-Galactosidases for Application of High-Temperature Processing of Soy Molasses
The $\alpha$-Galactosidases from the novel thermophilic strain KM-THCJ and hyperthermophilic Thermotoga neapolitana were examined for their potential use in processing of soy oligosaccharide waste by-products. The $\alpha$-galactosidase of strain KM-THCJ has been purified to homogeneity and has a …
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Enzymes Involved in the Utilization of the Galactomannan, Guar Gum, by Bacteroides Ovatus
… gum, a galactomannan, it produces an (alpha)-galactosidase (I) which is different from the (alpha)-galactosidase (II) it produces when it is grown on galactose, melibiose, raffinose or stachyose. I have purified both of these enzymes to apparent homogeneity. Both enzymes appear to be trimers …
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The identification of chemical compounds that decrease cellular levels of toxic Huntington's disease protein through a novel cell-based assay
… using the enzymatic activity of E. coli [beta]-galactosidase as a reporter for Htt protein levels. I expressed either expanded (97Q) or normal (23Q) Htt fused to the [beta]-galactosidase alpha-subunit ([alpha]) in an inducible fashion in PC12 cells which also expressed the [beta]-galactosidase …
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Lactose Operon Expression and Plasmid Rearrangement in Rhodopseudomonas Sphaeroides (Gram Negative Bacteria)
… Tn951, was examined. Very low levels of (beta)-galactosidase were observed in R. sphaeroides but mutants of this bacterium were recovered that were able to use lactose as the sole carbon source and which had elevated (beta)-galactosidase expression. The mutation allowing growth on lactose was …
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PROTEIN PURIFICATION FROM GASTROPODS
… β-mannosidase, α-glucosidase, β-glucosidase, β- galactosidase and β-N-acetylglucosaminidase) in 8 different species of snails (Arion lusitanicus, Biomphalaria glabrata, Achatina fulica, Limax maximus, Cepaea hortensis, Lymnea stagnalis, Arianta arbustorum, Planorbarius corneus and egg from …
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Probiotic metabolism of human milk oligosaccharides (HMOs) and prebiotics
… L. acidophilus NCFM and further investigated a -galactosidase gene involved in LNnT utilization by L. acidophilus NCFM. β-galactosidase lacL gene knockout in L. acidophilus NCFM and subsequent carbohydrate utilization analysis demonstrated that LNnT was unable to be utilized by the knockout …
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ΚΑΘΑΡΙΣΜΟΣ ΚΑΙ ΜΕΛΕΤΗ ΙΔΙΟΤΗΤΩΝ ΜΙΑΣ Β-ΓΛΥΚΟΣΙΔΑΣΗΣ ΚΑΙ ΜΙΑΣ Β-ΓΑΛΑΚΤΟΣΙΔΑΣΗΣ ΑΠΟ ΣΠΕΡΜΑΤΑ ΚΡΙΘΑΡΙΟΥ. ΕΦΑΡΜΟΓΗ ΤΩΝ ΕΝΖΥΜΩΝ ΣΤΗΝ ΥΔΡΟΛΥΣΗ ...
TWO ENZYMES A B-GLUCOSIDASE AND A B-GALACTOSIDASE WERE ISOLATED AND CHARACTERIZED FROM BARLEY SEEDS. THE B-GLUCOSIDASE IS A SINGLE BASIC POLYPEPTIDE (PI>8,5) WITH A MR OF 53.000 ACTING OPTIMALLY AT PH 4.5-5.0. THE B-GALACTOSIDASE IS COMPOSED OF TWO SUBUNITS WITH A MR OF 42.000 AND 33.000 …
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Use of bgaH as a reporter gene for studying translation initiation in the archaeon Haloferax volcanii
… isolated from Haloferax lucentensis codes for P-galactosidase. To study the function of initiator tRNAs in translation initiation in Haloferax volcanii, the initiator AUG codon of the bgaH gene was mutated to UAG, UAA, UGA, and GUC. Four different H. volcanii initiator tRNA derived mutants with …
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Improvement of engineered thermostable xylanase production via immobilization of recombinant escherichia coli onto graphene oxide
… graphene oxide (GO) on xylanase excretion and ~-galactosidase activity of immobilized E. coli was examined. The experiments were performed under the optimized RSM conditions (20°C, 0.5 mM, IPTG and pH 7) using shake flask cultivation followed by bioreactor conditions (20°C, 0.5 mM, IPTG and pH 7) …
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Modification of Vegetable Soybean Sugar Content via Gene Transformation
… with yeast invertase (SUC 2) and $\alpha$-galactosidase (agl A) from Aspergillus niger under the control of the lectin promoter using particle bombardment. The transformation was not very successful. In some cultivars, no green clumps were obtained after 8 weeks of selection in FG medium …
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