Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 179 for “"fusion proteins"”.
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ISPH fusion proteins, ISPH inhibition, and bisphosphonate mechanism of action
… class of bacterial IspH we called IspH-RPS1, a fusion protein of IspH with ribosomal protein S1 (RPS1), was characterized. Next, a new class of IspH inhibitors, one without diphosphate groups, was discovered. The characterization of an IspH-RPS1 began with a bioinformatics analysis of the IspH …
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Characterizing the Phosphorylation State of Tie2 using SH2 Domain Fusion Proteins
… and autophosphorylation, whereby adaptor proteins recognizing the phosphorylated tyrosine activate downstream signaling via their Src homology 2 (SH2) domains. Currently there are no phosphospecific antibodies for Tie2, therefore, identifying critical residues responsible for certain …
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Streptavidin-cytokine fusion proteins for use as adjuvants in cancer vaccines
… of recombinant streptavidin-cytokine fusion proteins. Immunoadjuvants can augment the weak or non-existent antitumour response of a patient with cancer to immunotherapy. The response of breast cancer patients to cancer vaccines directed against tumour associated antigens, such as …
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Development of SNAP-tag-based fusion proteins targeting HIV-1 viral reservoirs
… J3-ETA IT, this work sought to produce a J3-SNAP fusion protein by osmotic stress expression in the presence of compatible solutes in the periplasmic space of E. coli. SNAP-tag is a self-labelling protein that covalently binds benzylguanine (BG)-modified substrates in a 1:1 stoichiometric ratio. …
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High-throughput profiling of viral class I fusion proteins via mammalian antigen display
Viral infections, particularly epidemics and pandemics, pose a significant threat to global health, causing substantial morbidity, mortality, and socioeconomic disruption. Moreover, viruses can cause chronic disease long after initial infection. While modern technologies enable early outbreak …
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High-throughput profiling of viral class I fusion proteins via mammalian antigen display
Viral infections, particularly epidemics and pandemics, pose a significant threat to global health, causing substantial morbidity, mortality, and socioeconomic disruption. Moreover, viruses can cause chronic disease long after initial infection. While modern technologies enable early outbreak …
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Generation of Glioblastoma specific SNAP based antibody fusion proteins for future radiolabelling application
… of the GBM tumour microenvironment. SNAP-tag® fusion antibodies against the abovementioned TAAs were employed in this study. The fusion antibody is a recombinant antibody-based fusion protein created by fusing the SNAPtag®, a modified version of the DNA repair enzyme O6 -alkylguanine DNA …
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Generation and in vivo validation of multi-functional antibody-fusion proteins for cancer therapy
… characterization of three antibody-based fusion proteins for cancer therapy. The first project focused on Tripokin, a multi-specific immunocytokine that combines interleukin 2 (IL2) and tumor necrosis factor (TNF) with the L19 antibody. Tripokin demonstrated enhanced tumor targeting, …
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Establishment of recombinant antibody technologies allowing for the generation of SNAP-tag fusion proteins
… allowing for the generation of disease specific fusion proteins. Specifically, these fusion proteins consist of single-chain antibody fragments genetically fused to snaptag, allowing for the generation of recombinant ADCs that could be used as a drug delivery system carrying any BG-modified drug …
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Antibody engineering to evaluate binding, internalisation, and intracellular routing of tumour-targeting fusion proteins
… This study focused on a novel recombinant fusion protein format for ADCs using a single chain variable fragment (scFv) specific to chondroitin sulphate proteoglycan 4 (CSPG4), a tumour-associated antigen often overexpressed in TNBC. The scFv antibody derivative was genetically fused to a …
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Regulation Of Nkcc2 Trafficking By Vesicle Fusion Proteins Vamp2 And Vamp3 In The Thick Ascending Limb
… activity. However, little is known about the proteins that mediate NKCC2 trafficking. Inhibition of the vesicle fusion proteins VAMP2 and VAMP3 blunts the increase in surface NKCC2 expression and NaCl absorption in response to stimulation by cAMP. In other cells, VAMPs mediate fusion of …
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Design and synthesis of aryl hydrocarbon receptor fusion proteins for polyclonal antibodies production and cellular delivery
… mechanism, our lab has generated many human proteins using recombinant DNA technology. This thesis documents the design and synthesis of a number of proteins of the AHR deletion construct CΔ553. The bacterial expressed and purified fusion proteins could be utilized as antigen to …
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cholera Toxin B Subunit-Diabetes Autoantigen Fusion Proteins Modulate Dendritic Cell Function and T Cell Morphogenesis
… mechanisms responsible for CTB-autoantigen fusion protein suppression of diabetes autoimmunity, dendritic cells (DCs), a major class of antigen presenting cells (APC) involved in restoration of immunological tolerance, were differentiated from umbilical cord blood monocytes and incubated …
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Generation of recombinant SNAP-tag based antibody fusion proteins for immunotherapy of acute myeloid leukemia (AML)
… vectors for production of recombinant SNAP fusion proteins (FPs). Purification of the SNAP FPs was performed using immobilized metal affinity chromatography (IMAC). SDS PAGE and Western blot analysis were used to characterise the fusion proteins, facilitated by an N-terminal 10x Histidine …
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Novel fusion proteins based on recombinant lectins for delivery of a cytotoxic peptide specifically to cancer cells
… is covalent attachment of carbohydrates to proteins. At the cell surface, molecular recognition events involved in cancer metastasis are mediated by sugar moieties of glycoproteins. Evidence from both patient (histochemical analysis) and experimental work (tumour models) show that the …
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Developing novel SNAP tag-based antibody fusion proteins directionally conjugated to fluorophores for immunophenotyping of acute myeloid leukemia
… can be used to identify specific proteins or antigens upregulated in AML. In low and middle-income countries, particularly Africa, AML diagnosis is primarily based on leukemic cells or blast morphology, which has significant drawbacks in terms of providing accurate and timely …
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Fusion Proteins as Selective Inducers of Constitutive Activity and Tools to Discriminate the Biased Signaling Profile of β2 Adrenoceptor Ligands
… pathways. We characterized the behavior of our fusion proteins β2AR-Gαs and β2AR-βarr2 in HEK293 cells by measuring their constitutive activity, transducer recruitment, and pharmacologic modulation. Our fusion proteins show (a) steric hindrance from the reciprocal endogenous transducers, (b) …
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Development of potential immunodiagnostic & therapeutic techniques using SNAP-fusion proteins as tools for the validation of Triple-negative Breast Cancer
… of TNBCs. First functionalities of two scFv SNAP fusion proteins and one VHH SNAP fusion protein previously not having been described are provided through binding analyses on receptor positive tumour cell lines. This was achieved by in-silico design and molecular cloning of genetically fused …
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The study of ancient viral class II envelope fusion proteins within the viral retroelements of Nematoda and the nuclear plasmids of Dictyostelium
… encodes an ancient viral class II envelope fusion protein. Typically, retroviruses and related degenerate retrotransposons encode a hemagglutinin-like class I envelope fusion protein. A subset of Bel/Pao LTR-retrotransposons within the phylum Nematoda have acquired a phlebovirus-like env and …
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