Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 278 for “"fusion protein"”.
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Heterotrimeric coiled-coils as viral fusion protein mimics
… of two or more a-helices, is a ubiquitous protein structure that mediates a wide range of biological activity. It is characterized by a heptad repeat of amino acids that serve to form both the hydrophobic core and electrostatic interfaces of the coiled-coil. Previous work in our lab showed …
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Regulation of the Human Parainfluenza Virus (hPIV3) Fusion Protein
… purpose of this work was to determine how the fusion (F) protein is regulated with a focus on the heptad repeat B (HRB) region of the F protein located in the ectodomain, directly adjacent to the transmembrane domain. This region has been suggested to play important roles in the initiation of …
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Ricin B Chain-Insulin Fusion Protein Immunomodulation of Type 1 Diabetes
… human proinsulin (INS) ricin toxin B chain (RTB) fusion protein was expressed in <em>Escherichia coli </em>and purified for use in an animal inoculation experiment to test for the suppression of diabetes symptoms. Non-obese diabetic (NOD) mice were orally inoculated and boosted with purified …
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The Specific Role of the MLL CXXC Domain in MLL Fusion Protein Function
… in chromosome translocations which produce novel fusion proteins that cause leukemia. The CXXC domain of MLL is a cysteine rich DNA binding domain with specificity for binding unmethylated CpG-containing DNA. The CXXC domain is retained in oncogenic MLL fusions, and is absolutely required for the …
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Expression Of Cholera Toxin B Subunit-rotavirus Nsp4 Enterotoxin Fusion Protein In Transgenic Chloroplasts
… each encoding a viral capsid or nonstructural protein. The identification of a rotavirus nonstructural protein gene (NSP4) encoding a peptide, which functions both as a viral enterotoxin and as a factor involved in the acquisition of host cell membrane during virus budding from cells, provides …
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Production and preliminary characterization of a fusion protein comprising streptavidin and a cellulose-binding domain
… Cellulomonas fimi can be fused to heterologous proteins. The fusion proteins can be purified by affinity chromatography on cellulose or immobilized on cellulose (Ong et al., 1989b). Streptavidin, a protein produced by Streptomyces avidinii, binds to the water-soluble vitamin, D-biotin, with …
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R-RAS41A fusion protein purification and the cloning of a new RAS GEF like gene
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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Cytochrome c peroxidase : its role in nitrosative stress and purification as a GST-fusion protein
Cytochrome c Peroxidase (CCP) is a hemoprotein found in the intermembrane space of yeast mitochondria. One of its roles is to protect the cell against oxidative stress by reducing mitochondrial H 2 O 2 to H 2 O. Titration of CCP with peroxynitrite [ONOO(H)] formed a species with an absorption …
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Validation of an aASPH-SNAP-tag fusion protein for immunodiagnosis of triple-negative breast cancer
Breast cancer is a serious health concern for women from as young as 20 years old and to this day is well known to be the leading cause of cancer incidences and mortalities in females. This disease is known as a heterogeneous disease that requires different systemic therapies, therefore early …
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The Mitochondrial Fusion Protein, Optic Atrophy 1 (OPA1), Alters Cellular Metabolism to Support Cancer Cell Viability
… remodelling processes: fission (division) and fusion (elongation). The inner mitochondrial membrane GTPase, OPA1, is a cristae-organizing protein that directly organizes formation of the electron transport chain for oxidative phosphorylation and thereby further coordinates with the …
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Design and development of an RNA vaccine against tumor-mediated angiogenesis and metastasis targeting a KLH-Adrenomedullin fusion protein
… and tested a mRNA vaccine directed against a fusion antigen composed by a small piece of keyhole limpet hemocyanin (KLH), as a hapten, and mouse AM. The in vitro-synthesized mRNA was encapsulated in lipid nanoparticles (LNPs) and injected in C57BL/6J mice. Empty LNPs were used as a negative …
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Characterization of the Biological Activities of Recombinant Fusion Protein Green Fluorescent Protein/Human Zona Pellucida Protein 3 (GFP/HZP3)
… the application of a pEGFP expression vector, fusion protein GFP/ZP3 was successfully generated and expressed. The expression of GFP/ZP3 was evidenced by RT-PCR and western blot. The fusion protein was partially purified by Ni-NTA affinity column from cell culture medium of stably transfected …
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Comparison of the therapeutic efficacy of SNAP-tag fusion protein and the synergistic actions of chemotherapy and photodynamic therapy in killing resistant melanoma
… potential, and aberrant expression of membrane proteins known as ABC transporters (ABCB5 and ABCG2), which mediate cellular resistance by extruding cytotoxic molecules from cells. To palliate these adverse effects, this study primarily aimed to investigate the efficacy of the synergistic actions …
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Complementation of an AtCAM3 knockout line restores wild type root growth and provides localization data through expression of a chimeric GFP:AtCAM3 fusion protein in Arabidopsis thaliana
… main isoforms of Calmodulin (CaM), one group of proteins that bind Ca2+ ions and interact with a multitude of protein targets to modulate their activity. These four CaM isoforms are encoded in seven different genes. An additional fifty calmodulin-like proteins (CMLs) also are present in the …
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Expression of a tick toxin for the development of a canine vaccine
… vaccine, Masina (1999) used a maltose-binding protein (MBP) holocyclus toxin (HT-1) fusion protein as immunogen. It was partially protective against challenges with crude tick extract in neonatal mice but unable to protect dogs against paralysis caused by direct tick attachment. Subsequently, …
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The study of ancient viral class II envelope fusion proteins within the viral retroelements of Nematoda and the nuclear plasmids of Dictyostelium
… encodes an ancient viral class II envelope fusion protein. Typically, retroviruses and related degenerate retrotransposons encode a hemagglutinin-like class I envelope fusion protein. A subset of Bel/Pao LTR-retrotransposons within the phylum Nematoda have acquired a phlebovirus-like env and …
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Towards Gene Therapy of Parkinson’s Disease: Implantation of RUC-GDNF Secreting Mammalian Cell Lines into Rat Brain
… cell line derived neurotrophic factor (RUC-GDNF) fusion protein in CSF. The RUC-GDNF protein is expected to report GDNF secreted from the cell by bioluminescence through luciferase activity.</p> <p>Current <em>ex vivo</em> gene therapy using GDNF is limited by lack of a monitoring mechanism to …
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Antibody engineering to evaluate binding, internalisation, and intracellular routing of tumour-targeting fusion proteins
… This study focused on a novel recombinant fusion protein format for ADCs using a single chain variable fragment (scFv) specific to chondroitin sulphate proteoglycan 4 (CSPG4), a tumour-associated antigen often overexpressed in TNBC. The scFv antibody derivative was genetically fused to a …
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Estudos de imunobioinformática e imunoprofilático de uma proteína de fusão contra a infecção por Corynebacterium pseudotuberculosis em modelo murino
… work was to evaluate in silico and in vivo the fusion protein MBP:PLD:CP40 as a new immunoprophylactic target for CLA. In silico analysis were performed using immunobioinformatics tools for the construction of the amino acid sequence, assembly and validation of the 3D structure, physicochemical …
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