Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"fusion assay"”.
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USING SINGLE VIRION FUSION ASSAY TO STUDY INFLUENZA VIRUS ENTRY
… change of HA that induces membrane fusion of the viral and endosomal membranes. Fusion allows the virus to release viral RNA into the cytosol of the cell. Single particle tracking (SPT) enables the study of viral binding and membrane fusion at the single-virion level to obtain high …
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Regulation of SNARE-dependent fusion in an in vitro system
… responsible for the execution of membrane fusion. Central to this machinery are members of the SNARE (soluble NSF attachment protein receptor) family of proteins. The process of SNARE-mediated membrane fusion needs to be tightly regulated and the SNARE proteins are partially responsible for …
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Regulation of membrane fusion by Tlg2p & Vps45p through the endosomal system of Saccharomyces cerevisiae
… of the machinery that facilitates membrane fusion in eukaryotic cells. SNARE proteins are subject to multiple levels of regulation, one of which is imbedded in the syntaxin (Qa-SNARE) molecule. It has been demonstrated that some syntaxins adopt a closed conformation, whereby an autonomously …
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Investigating Spatiotemporal Kinetics, Dynamics, and Mechanism of Exosome Release
… We visualized and characterized single MVE fusion events in lung cancer cells (A549) using CD63 fluorescent probes and total internal reflection fluorescence (TIRF) microscopy. The kinetics of release, or loss of fluorescence post-fusion, can relay information about the fate of exosomes. …
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DEVELOPMENT OF ANTAGONISTS TARGETING CHEMOKINE RECEPTOR CCR5 AND THE CHEMOKINE RECEPTOR CCR5 – MU OPIOID RECEPTOR HETERODIMER
… was studied with radio-ligand binding assays, functional assays, HIV-1 fusion assays, cell fusion assays, and in silico molecular dynamics. The subsequent bivalent ligand was proven to be a potent inhibitor in both an artificial cell fusion assay mimicking HIV invasion and a native …
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The barrier functions of the cervical mucus plugs and purified mucins against a panel of HIV-1 strains in the contexts of cell-free and cell-to-cell infections
… infection in an in vitro cell-free inhibition assay. We aimed to characterize and compare the inhibition of cell-free and cell-associated HIV-1 strains by the crude cervical mucus plugs and purified cervical mucins. Fifty-two cervical mucus plugs were collected from women in labour in 6 M …
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FMF assay for assessing vaccine generated antibodies in a biomimetic manner
Traditional functional assays such as hemagglutination inhibition (HAI) and micro-neutralization (MN) assays have been routinely used for assessing the vaccine response, since influenza vaccine has been administered in people (1940). Such assays are not always predictive regarding the protection …
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Cellular Mechanisms of Resveratrol's Interaction with Mitochondrial Reactive Oxygen Species Metabolism
… confocal microscopy and a novel ‘cell free’ fusion assay it was determined that concurrent with changes in MnSOD protein levels, resveratrol treatment leads to a more fused mitochondrial reticulum. This observation may be important to resveratrol’s ability to slow proliferative cell growth …
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Novel Non-Repulsive Outcomes From Ephrin-B Reverse Signaling
… epithelia. Finally, an in vitro palatal shelf fusion assay used to determine the role of Eph-ephrin signaling in these developmental events indicates clear roles for ephrin-B2 reverse signaling in cell-cell adhesion. Taken together, my data leads me to propose that ephrin-B reverse signaling is …