Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"furin"”.
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Cryo-electron microscopy of HPV16 pseudovirions reveal changes in capsid conformation upon furin cleavage
… (HSPGs). Cleavage of L2 by a host protease, furin, is necessary for infection and is thought to facilitate a conformational change in the virus capsid. Furin cleavage may affect the ability of HPV to bind to sulphated glycoproteins and a HSPG substitute, heparin. Understanding these proposed …
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In Vitro Characterization of Proglucagon Cleavage by PCI
… and monobasic residues. Identification of a furin/propeptide convertase (furin/PC) family of endoproteases has led to in vivo and in vitro studies aimed at understanding the mechanism of selective precursor processing at basic residues.|To understand how enzymes function, their kinetic …
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Einfluss posttranslationaler Modifikationen auf die Funktion des Prototyp Foamy Virus Hüllproteins
… oder dem trans-Golgi Netzwerk, durch Furin oder eine Furin-ähnliche Protease in drei partikelassoziierte Untereinheiten prozessiert. Eine Partikelassoziation retroviraler Signalpeptide ist bislang nur für Foamyviren nachgewiesen worden, genauso wie eine essentielle Rolle dieses …
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Charakterisierung der Prototyp Foamyvirus Hüllglykoprotein Rezeptorbindungsdomäne
… dieser Arbeit wurde der Beweis erbracht, dass Furin oder Furin-ähnliche Proteasen und nicht der Signalpeptidase-Komplex für beide proteolytischen Spaltungen verantwortlich sind. Durch die N-terminale Sequenzierung der SU- und der TM-Untereinheit eines aufgereinigten PFV …
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Investigating the Role of Dispatched in Hedgehog Ligand Transport and Delivery
… effort in which we discovered that the Furin family of proprotein convertases facilitate the cleavage of Disp at a conserved site of the first extracellular loop. The Furin family has been implicated in cleaving ligands and receptors of other developmental signaling pathways, but no …
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Channel activating proteases: molecular targets for correction of sodium channel dysfunction in cystic fibrosis airways disease
… (AECs). Further, two highly selective and potent furin inhibitors, BOS-981 and BOS-318 were characterised, with the aim of determining whether selective inhibition of furin has the potential to reduce ENaC-mediated sodium transport and improve airways hydration. The effect of BOS-981 and BOS-318 …
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Charakterisierung von reptilienpathogenen Paramyxoviren und Analyse des prokaryotisch exprimierten partiellen Fusionsgens
… predicted to be glycosylated and to contain a furin cleavage site in the F1 und F2 subunit. Domains were predicted to be the signal-peptide (SP), a hydrophobic fusion peptide, two heptad repeats (HR A and HR B), a leucine zipper motif, and a transmembrane anchor (TM). To further characterize …
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Somatic cell gene therapy for diabetes mellitus: engineering a surrogate B-cell
… cleavage by the subtilisin-like serine protease, furin, was carried out. Co-transfection of human fibroblasts with wild-type proinsulin and furin resulted in 58% conversion to mature insulin by these cells. Intraperitoneal implantation of the mature-insulin secreting human fibroblasts into the …
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CHASING VARIANTS: MAKING THE MATCH BETWEEN THE SARS-COV-2 S1/S2 CLEAVAGE SITE AND HOST CELL PROTEASES
… site, which is thought to be mainly cleaved by furin during virion formation. The 681st amino acid S position in particular has been the site nonynomymous substitutions several times, and interestingly has toggled between arginine (R) and histidine (H) ever since leaving the original proline (P) …
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Characterisation of Prodomains in the GDNF Family
… proteolysis. GDNF family members are cleaved by furin, with a furin site between the pro and mature domains. Prodomains are known to alter TGF-β protein properties including reducing bioactivity and enhancing half-life. To determine the impact of the prodomain on signalling, the different protein …
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Identifikation des Transportproteins PACS-1 als Nephrocystin-interagierendes Protein
… Interaktion von PACS-1 mit Nephrocystin die sog. Furin-Bindungsregion von PACS-1 und das erste Acidic Cluster von Nephrocystin benötigt und sowohl in transfizierten Zellen als auch in der Niere mit nativen Proteinen nachweisbar ist. Interessanterweise wird die Interaktion reguliert durch …
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Spike Processing and Protease Usage Effects on SARS-CoV-2 Pathogenesis
… that are unusual to sarbecoviruses – the furin cleavage site (FCS) and the QTQTN motif. In this dissertation, we demonstrate that the CTS1 is more complex than previously thought. We have established the aspects of cleavage site efficiency, differential protease usage, loop length, and …
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Whole-exome sequencing variant prioritization in individuals with tense and agreement marking deficit— a clinical marker of specific language impairment
… lists to suggest six new genes (PDHA2, PCDHB3, FURIN, NOL6, IQGAP3, and BAHCC1) possibly involved in SLI, and evidence supporting two genes previously reported as genes likely contributing to overall language ability (GLI3 and FLNB). Importantly, the results show selective prioritization of …
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The structure of human pro-myostatin and molecular basis of latency
… Following cleavage of the prodomains by furin, pro-myostatin persists as a stable non-covalent complex which is resistant to the natural inhibitor follistatin and exhibits significantly weaker bioactivity than the mature growth factor. A number of distinct structural features combine to …
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Receptor Mediated Oral Delivery Of Bioencapsulated Green Fluorescent Protein Expressed In Transgenic Chloroplasts
… (CTB), a CTB-GFP fusion protein separated by a furin cleavage site was expressed via the tobacco chloroplast genome and used as a visible marker. Site specific integration of the transgene was confirmed by PCR analysis. Southern blot analysis confirmed homoplasmy. Immunoblot analysis confirmed …
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DEVELOPMENT OF AVIAN PARAMYXOVIRUS VECTORED VACCINES
… NDV vector, with a 2A self-cleaving peptide, a furin cleavage site, or both, placed between the AIV and NDV sequences for separation of the two proteins. Among different constructs tested, we only recovered viable viruses with AIV HA fused C-terminally to the NDV HN gene. These viruses …
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Characterization of antigen processing and presentation by peptide-linked MHC class I molecules
… trans-Golgi-Netzwerk exprimierten Endoprotease Furin bei der Prozessierung unserer membranassoziierten Modellproteine finden. N-terminale Verlängerungen des SIINFEKL-Epitops und verschiedene C-terminal flankierende Aminosäuren wurden in der Regel effizient entfernt, wobei Prolin als flankierende …
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Investigation of particulate HIV-1 Env vaccine candidates using Zera® and SpyTag/SpyCatcher technologies
… contain a flexible linker (FL) in place of the furin cleavage site and an I559P mutation to generate soluble, stable and cleavageindependent gp140 proteins. The first approach investigated the impact of genetically fusing a 27 kDa proline-cysteine-rich domain of the ɣ-zein maize seed storage …
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Characterization and Functional Analysis of a Newly Identified Human MT5-MMP Transcript Variant Isolated from Multipotent NT2 Cells
Membrane-type 5 matrix metalloproteinase (MT5-MMP) is unique among MMP family members as it is predominately expressed in the CNS. Its expression is ubiquitous during brain development and restricted to regions of neurogenesis and neuroplasticity in the adult. MT5-MMP is a mediator of pericellular …
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Generation and characterization of HIV-1 subtype C candidate vaccines that will induce high titre antibody responses to HIV-1 envelope glycoprotein
… the tissue plasminogen activator leader, the furin cleavage site with a glycine rich flexible linker, and to introduce an I559P mutation. DNA and modified vaccinia virus Ankara (MVA) vaccines were generated where Env was truncated to gp150, thereby retaining the transmembrane domain and a …