Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"functional screening"”.
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Natural Products From Functional Screening of Soil Metagenomic Libraries
… with desired characteristics and activities. Functional screening uses phenotypically identifiable traits such as changes in color or antibiotic activity to discover clones of interest in a metagenomic library. This mode of screening is contingent upon the bacterial host being able to …
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Endogenous Reporter Systems for High-Throughput Functional Screening in Clear Cell Renal Cell Carcinoma
… and chromatin regulators for high-throughput screenings. Our screening data was analysed through a permutation analysis to remove false positive hits. In the HIF2A reporter screen with the TF library and the chromatin library, HIF2A was the sole significant hit, suggesting redundancy within …
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Construction and phenotypic screening of mid-size insert marine microbial environmental genomic libraries
Functional screening of environmental genomic libraries permits the identification of clones expressing activities of interest without requiring prior knowledge of the genes responsible. In this study, protocols were optimized for the construction of mid-size DNA insert, inducible expression …
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Characterisation, bioactivity evaluation and rational modification of bioactive peptides from frog skin secretion and scorpion venom
… with antibacterial and anticancer activities. Functional screening and exploration of structure-activity relationship in this work would help in a great understanding of naturally-occurring disulphide-bridged peptides. These bioactive peptides are likely to become potent candidates for novel …
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The membrane-active antimicrobial peptide from frog skin and scorpion venom: from mechanistic insight to design
… in frog Hylarana guentheri, skin secretion. Functional screening revealed that brevinin-1GHd exhibited potent antimicrobial, antibiofilm, and anticancer efficacy without inducing obvious haemolysis and cytotoxicity at working concentrations, which made it promising to be further developed as …
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Identification and Characterization of MicroRNAs Modulating Cardiac Hypertrophy
… a high-content microscopy, high-throughput functional screening for human microRNAs able to reduce neonatal cardiomyocyte (CM) cell size using a whole-genome microRNA library. The most effective anti-hypertrophic microRNA was hsa-miR-665. In a model of transverse abdominal aortic …
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ADAP1 Promotes Latent HIV-1 Reactivation by Tuning the KRAS-ERK-AP-1 Signaling-Transcriptional Axis
… regulators. Specifically, I implemented a functional screening platform, given HIV-1 gene expression relies on CD4+ T cell activation state, to identify host factors modulating CD4+ T cell signaling-transcriptional axes and consequently HIV-1 fate. Among the hits, I focus on ADAP1 (ArfGAP …
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Microglial Modulation of Neuronal Autophagy: Uncovering Cytokine-Mediated Pathways
… direct analysis of secreted cytokines and functional screening via knockout of neuronal cytokine receptors. However, no negative regulators of autophagy were found. Instead, two receptor-ligand pairs, CXCR5-CXCL13 and CXCR3-CXCL10, were identified as inducers of autophagy. CXCL13, the …
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Functional metagenomic analysis of carbohydrate degrading enzymes from the human gut microbiota
… project, a culture-independent approach based on functional screening of genomic and metagenomic libraries using Escherichia coli and Lactococcus lactis as heterologous expression hosts, was used to isolate novel genes encoding glycoside hydrolase (GH) enzymes. The study identified several active …
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The involvement of microglial activation in schizophrenia
… in circulating blood. We applied a high-content functional screening platform, to characterize alterations in microglial intracellular signalling cascades induced by schizophrenia patient serum relative to control serum. Using automated sample preparation, fluorescent cellular barcoding and flow …
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Novel reaction discovery with rapid high-throughput experimentation via infrared spectroscopy and enzymatic electrochemical oxidation of alcohols
… sustainability and productivity. High-throughput screening (HTS) and high-throughput experimentation (HTE) has been widely used in the field of biochemistry to rapidly identify pharmacological targets. However, this method has been underused in organic chemistry due to the complexity of reaction …
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Engineering structural variants with prime editing
… a challenge for large-scale applications and functional screens. In this thesis, I explore features determining prime editing efficiencies for both insertions and long deletions. After demonstrating that paired prime editing screens work efficiently across reporter locations, I scaled paired …
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Improving the identification and management of Binge Eating Disorder and Bulimia Nervosa in adults aged 16 and over in primary care in the United Kingdom
… patients, their families and the public, (2) functional screening pathways embedding suitable and accurate screening tools need to be developed, (3) current referral and treatment pathways must be improved to simplify referral processes and provide interim management options for patients, and …
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Development of a Functional Movement Screening Tool for Football
… a risk of injury during participation. Therefore screening procedures are important not only to reduce the risk of injury but also for developing subsequent injury prevention programmes. Assessing functional movement is vital in screening in order to examine an athlete’s ability to perform …
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Investigation of the GATOR1 complex genes in focal cortical dysplasia and focal epilepsy
… major pathogenic mechanism. To investigate the functional outcomes of specific patient variants of all three GATOR1 genes, we develop a functional assay using GATOR1 null cell lines, measured by the ability to rescue of null phenotypes in vitro and in vivo contexts. We confirm that some variants …
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Ultrahigh-Throughput Microfluidic Droplet Screening of Metagenomic Libraries for Esterases and Kemp Eliminases
In the search for new enzymes, functional metagenomic libraries are particularly interesting since they give access to the genomes of the 99% of microorganisms which cannot be cultured in the laboratory. However, using classical biochemical assays to screen such libraries is impeded by the fact …
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High-throughput protein engineering tools for efficient exploration and quantitative mapping of protein fitness landscapes
… and isolation of desired phenotypes through screening or selection, has had a profound impact on society. It has led to the development of key pharmaceuticals, precision gene editing tools that target previously incurable diseases, and biocatalysts for the production of active pharmaceutical …
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Expanding the toolbox for precision medicine with silicon photonic microring resonators and microfluidic technologies
… formed from patient samples could be used for functional screening of membrane proteins in response to targeted therapeutics. Finally, interfacing the microring resonator platform with on-chip electrophoresis could prove to be a remarkably useful combination. The microring resonator platform …