Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 17 of 17 for “"functional genetics"”.
-
Functional genetics reveals surprises about the mechanisms of action of chemotherapeutics
… for some patients and not others. Here, I used functional genetics to examine the mechanisms of action of new and emerging anti-cancer agents. First, the platinum-based chemotherapeutics, cisplatin and its analogues, carboplatin and oxaliplatin, were evaluated. Previously all platinum …
-
Design of a microscopy-based screening method for stomatal development genes in <i>arabidopsis thaliana</i>
… purpose of this project was to create a reverse genetics, microscopy-based screening method to identify Arabidopsis genes important in guard cell development. It was hypothesized that gene coexpression analysis would facilitate identification of gene candidates involved in stomatal development. …
-
Sensory divergence among populations of a southern African endemic horseshoe bat (Chiroptera: Rhinolophidae ): a multidisciplinary approach
… combining the methods of sensory ecology, functional morphology, population genetics and functional genetics was used to explore the relative roles of neutral and adaptive processes in the evolution of sensory divergence in Cape horseshoe bats, Rhinolophus capensis. Geographic variation in …
-
Ecological genetics of the moss Physcomitrella patens
… advanced the body of knowledge surrounding plant functional genetics and molecular biology, yet very little is known about the ecology and population genetics of this species. Although the bryophytes are the second largest group of plants, there is little information regarding the population …
-
Development of a Transposon Based Activation Tagged Mutant Population in Tomato for Functional Genomic Analysis
… sequencing in progress, there is a need to study functional genetics through mutant lines that exceed the practical limitations imposed by the popular research cultivar, Micro-Tom. This project utilized Agrobacterium transformation and the transposon tagging construct, Ac-DsATag-Bar_gosGFP, to …
-
A combinatorial approach to scientific exploration of gene expression data: An integrative method using Formal Concept Analysis for the comparative analysis of microarray data
Functional genetics is the study of the genes present in a genome of an organism, the complex interplay of all genes and their environment being the primary focus of study. The motivation for such studies is the premise that gene expression patterns in a cell are characteristic of its current …
-
Multicellular Phenotypic Studies of Single Gene Variants in Myxococcus xanthus
… This dissertation presents a systematic study of functional genetics and related phenotypes using quantitative methods. The first part of this dissertation will report the successful identification and characterization of 28 genes in the multicellular bacterium <em>Myxococcus xanthus</em> using …
-
LINKING TRANSCRIPTIONAL AND MORPHOLOGICAL HETEROGENEITY TO THERAPEUTIC VULNERABILITIES IN GLIOBLASTOMA
… approaches for the integration of molecular and functional-genetics data from preclinical models with the aim of prioritizing subtype-specific therapeutics vulnerabilities in Glioblastoma (GBM). Particularly, in an initial phase of my activities, I have designed and implemented computational …
-
Genome engineering and functional gene regulation tools for the study of malaria parasites
… by the lack of robust tools for performing functional genetics in P. falciparum. The identification and characterization of essential functions for parasite survival are fundamental steps towards the creation of effective antimalarial therapies. In this work, we developed an integrated set …
-
Investigating the functional effects of genes involved in genital divergence between Drosophila species
… conducted. It was found that males with only a functional copy of the D. mauritiana trn allele, that carry larger claspers with more sensory bristles, copulate for significantly less time than those with the functional D. simulans trn allele. Males with a functional D. mauritiana sox21b allele, …
-
Function and evolution of seminal fluid components in Drosophila
… Despite this rapid turnover, conservation of functional classes is frequently seen, suggesting evolutionary constraint on fundamental reproductive processes. One such functional class of SFPs is the family of odorant binding proteins (Obps), which are common members of the arthropod ejaculate. …
-
The complete plastid genome sequence of Passiflora cincinnata: genome rearrangements, massive plastid gene losses and implications to genome-plastome incompatibility
… rpl22, ycf1 and ycf2), which very likely were functionally transferred to the nucleus and its products are imported into plastid. This genome also showed a high rate of nucleotide substitution in several genes, such as accD and clpP. Despite this high divergence, the translated amino acid …
-
Rapid diversification and wing colour pattern evolution in tropical butterflies
… fieldwork to population genomics analyses, phylogenetics and genome-wide association studies, to functional genetics such as CRISPR-Cas9 genetic modification, RNAseq, in situ hybridization and antibody staining. I resolve the classification of these notoriously taxonomically challenging …
-
Identification and Characterization of Antibiotic Biosynthetic Gene Clusters in Pantoea
… suggesting the PNP-3 biosynthetic clusters are functional, and all strains carrying a pnp3a homolog, regardless of genomic context, were tolerant to PNP-3. These results suggest Pnp3a plays an essential role in PNP-3 export and resistance. Chapter four describes an antibiotic production survey …
-
Computational analysis and method development for high throughput transcriptomics and transcriptional regulatory inference in plants
… mechanisms, RNA-seq has been extensively used in functional genetics and transcriptomics, system biology and developmental biology in animals, plants, diseases, etc. The aim of this project is to use mathematical and computational models to integrate big genomic and transcriptomic data from …
-
Application and Development of Advanced Genetic Tools to Study Adult Stem Cells
… of the abovementioned genes using in vitro functional genetics I identified gaps in the current technology for organoid genetic engineering. I therefore developed two gene editing methods; a gRNA concatemer system allowing simultaneous knockout of multiple genes and CRISPR-FLIP enabling …
-
Targeted Deletion of Functionally Validated Enhancers Defines Their Role In Mouse Limb Development
<p>Transcriptional enhancers are genomic DNA sequences that contain clustered transcription factor (TF) binding sites. When combinations of TFs bind to enhancer sequences they act together with basal transcriptional machinery to regulate the timing, location and quantity of gene transcription. …