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Showing 1 to 2 of 2 for “"functional buffering"”.
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Biases and Blind-Spots In Genome-Wide Crispr-Cas9 Knockout Screens
… to mammalian cells revolutionized the field of functional genomics, enabling genome-scale genetic perturbations to study essential genes, whose loss of function results in a severe fitness defect. There are two types of essential genes in a cell. Core essential genes are absolutely required for …
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Utilizing the IN4MER CRISPR/CAS12A Multiplex Knockout Platform To Investigate Synthetic Lethality in the Human Genome
… Cas9-based single knockout libraries due to functional buffering. The Cas12a enzyme, unlike Cas9, can intrinsically process multiple guide RNAs from the CRISPR array, only requiring direct repeats (20 nucleotides), instead of tracrRNA (76 nucleotides) in Cas9 systems, thus freeing up space to …