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Showing 1 to 20 of 344 for “"fret"”.

  1. Study Of Protein-Rna Interactions Using Fluorescence Resonance Energy Transfer (fret) And Single-Molecule Fret

    Lamichhane, Rajan2011

    … Fluorescence Resonance Energy Transfer (smFRET) techniques that provide information about heterogeneity and dynamic behaviors of molecules have been developed to investigate inter- and intra-molecular interactions. Here we have used FRET in combination with smFRET to study three very …

    wayne-thes Repository record

  2. Magreceptorok ligandkötési affinitásának vizsgálata FRET szenzorral

    Kovács, Dávid2017

    … eredményei alapján kiszámítottuk az átlagos FRET hatásfok értékeket az egyes mintákban. A különböző ligand koncentrációkkal kezelt sejtekben nem volt egyenes arányosság a ligand koncentráció és a FRET hatásfok között. A plazmid konstruktokban túl hosszú a linker szakasz az LBD-k és az mCherry …

    debrecen Repository record

  3. Fluorescence Resonance Energy Transfer (FRET) Based Sensors for Bioanalysis

    Blagoi, Gabriela

    … based on fluorescence resonance energy transfer (FRET) for bioanalysis. Chapter 3 describes the use of FRET between donor fluorophores and acceptor labeled murine macrophage cells. FRET microscopy was used to determine whether the donor molecules truly permeate through the cell membrane or only …

    uno Repository record

  4. Fret Studies of DNA Binding By Lambda Cro Variants

    Wang, Lei2019

    … by fluorescence resonance energy transfer (FRET). Single-chain Cro dimer binds to operator DNA more than 100-fold faster and tighter than wild type Cro. A secondary mutation (scCro(PFPS-AWSG) ) that replaces two proline residues in the context of scCro reduces its equilibrium affinity for …

    mississippi Repository record

  5. Functional Aspects of Protein Kinase C FRET Probe Performance

    Jost, ChristianeUniversität Heidelberg

    … factors influencing the performance of two FRET based protein kinase C (PKC) probes, KCP-1 and KCP-2. KCP-1 consists of the truncated PKC substrate pleckstrin, sandwiched between two fluorescent proteins, EYFP and GFP2. KCP-2 is a shortened version of KCP-1, missing the last 18 amino acids …

    heid-diss Repository record

  6. Optymalizacja metod analizy Főrsterowskiego rezonansowego przeniesienia energii (FRET) pojedynczych cząsteczek

    Hajdziona, Marta2011

    amu-pl

  7. Fluorescence Resonance Energy Transfer (FRET) systems for biomedical sensor applications

    Bird, AoibheannDublin City University2010

    … use of Fluorescence Resonance Energy Transfer (FRET) for biomedical sensor applications. FRET is a process by which energy is transferred, via long range dipole-dipole interactions, from a donor molecule (D) in an excited electronic state to an acceptor molecule (A). The emission band of D must …

    dcu Repository record

  8. FRET-Based Conformational Sensor for the m1 Muscarinic Cholinergic Receptor

    Chang, Seungwoo2012

    … fluorescence resonance energy transfer, FRET, to monitor activation-associated conformational changes in intracellular loop 3 of the receptor. In the sensor, a CFP FRET donor and a labeling site for FlAsH (fluorescein-based biarsenical dye) FRET acceptor are engineered into the M1 …

    utswmed Repository record

  9. Single-molecule FRET analysis of the dynamics of AMPA receptor

    Salatan, Ferandre C.Rice University2010

    … fluorescence resonance energy transfer (smFRET) spectroscopy has advanced to become a powerful tool in characterizing biomolecules to the molecular level. In this thesis, the smFRET technique was utilized for the first time to analyze the dynamics of the conformational changes of the …

    rice Repository record

  10. Veranschaulichung subzellulärer physikalischer Kräfte biochemischen und mechanischen Ursprungs mittels FRET

    Mitkovski, Miso2012

    … des Förster Resonanz Energie Transfers (FRET) wurde ein optischer Biosensor konstruiert, um den Umfang aktiver, GTP-gebundener RhoA GTPase zu veranschaulichen, deren biochemiche Aktivität zur Entstehung physikalischer Kräfte im zellulären Cytoskelett führt. Der Biosensor wurde durch den …

    goettingen Repository record

  11. Single-molecule FRET study on the RecA-mediated DNA repair

    Joo, Chirlmin2012

    The RecA protein helps maintain genomic integrity through recombination. Using single-molecule assays and hidden Markov modeling, we show the most direct evidence that a RecA filament grows and shrinks primarily, one monomer at a time only from the extremities. Both ends grow and shrink, contrary …

    uiuc Repository record

  12. Force manipulation and single molecule FRET of transcriptional regulatory factors

    Brenner, MichaelUniversity of Illinois at Urbana-Champaign2013

    Made available in DSpace on 2013-02-03T19:17:18Z (GMT). No. of bitstreams: 2 Michael_Brenner.pdf: 48140044 bytes, checksum: 737009cfe6f7c863e8be1a53bd84ce23 (MD5) license.txt: 4065 bytes, checksum: 7b3880bb6f2daecb451ca65aadeab0b2 (MD5)

    uiuc Repository record

  13. Förster resonance energy transfer (FRET)-based nanophotonics using DNA origami structures

    Olejko, LydiaUniversität Potsdam

    … molecule by Förster resonance energy transfer (FRET). The efficiency of this energy transfer is highly dependent on the donor-acceptor distance. Hence, in these nanoscale photonic devices it is of high importance to have a good control over the spatial assembly of used fluorophores. Based on …

    potsdam-diss Repository record

  14. Single Molecule Fret Studies of DNA Binding and RNA Polymerizing Proteins

    Rahul, Roy2011

    Submitted by Megan O'Donnell (mnodonn2@illinois.edu) on 2011-11-07T16:47:35Z No. of bitstreams: 1 2007_roy_biophysics.pdf: 6758862 bytes, checksum: 83953b72a305a817a6a698b6e9d6fb86 (MD5)

    uiuc Repository record

  15. Fret-Based Investigations of The Structure-Function Relationships In The Nmda Receptor

    Dolino, Drew M, <p>0000-0002-2690-7140</p>2017

    … molecule Förster Resonance Energy Transfer (smFRET) as a means to address that gap. We have examined the question of partial agonism of the NMDA receptor, noting a mechanism of a dynamically graded cleft closure. We have pushed the bounds of the temporal resolution of such methods and been able …

    uthsc Repository record

  16. Quantenpunktbasiertes spektroskopisches Lineal mit Terbium-Komplexen als Donoren für optische FRET-Multiplexmessungen

    Morgner, FrankUniversität Potsdam

    Der Förster-Resonanzenergietransfer (FRET) liefert einen wichtigen Beitrag bei der Untersuchung kleinskaliger biologischer Systeme und Prozesse. Möglich wird dies durch die r-6-Abhängigkeit des FRET, die es erlaubt Abstände und strukturelle Änderungen weit unterhalb der Beugungsgrenze des Lichts …

    potsdam-diss Repository record

  17. Erweiterung eines Einzelmolekül-FRET-Spektrometers um alternierende Laseranregung und Charakterisierung des Systems

    Kaudse, TillmannUniversität Heidelberg

    Förster-Resonanzenergietransfer (FRET) ist ein Effekt, bei dem die Energie eines durch einen Laser angeregten Fluoreszenzfarbstoffs (der Donor) strahlungsfrei auf einen zweiten (den Akzeptor) übertragen wird. Da die Effizienz dieser Energieübertrag vom gegenseitigen Abstand der beiden Farbstoffe …

    heid-thes Repository record

  18. Acquisition and analysis of DNA recombination intermediates using single molecule FRET microscopy

    McKinney, Sean Aaron2012

    Submitted by Meng Tao (mengtao2@illinois.edu) on 2012-10-23T20:45:46Z No. of bitstreams: 1 McKinney_Sean.pdf: 2035766 bytes, checksum: 414180e8b31f044c02a9ee30c58f9b8f (MD5)

    uiuc Repository record

  19. Single molecule fret study on the mechanism of RecA mediated strand exchange

    Madabhusi Ragunathan, KaushikUniversity of Illinois at Urbana-Champaign2012

    … RecA filament can explain the rapid changes in FRET which we have observed upon the docking of non-homologous dsDNA to the RecA filament. We further show that homology can be identified during such sliding. Sliding is thermally driven and occurs in the absence of ATP hydrolysis. Furthermore, …

    uiuc Repository record

  20. A FRET Investigation into Molecular Mechanisms of Cardiac Troponin Activation in Reconstituted Thin Filaments

    Moutsoglou, Maria Eleni2016

    … A multi-site Förster resonance energy transfer (FRET) assay in cardiac Tn in reconstituted thin filaments was used to investigate the Ca2+-dependent structure and dynamics of the SABS, and show current theories behind Tn activation are biased using structures developed in isolated Tn. The SABS …

    sdstate Repository record

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