Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 80 for “"frameshift"”.
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Investigating the functional roles of the viral frameshift protein 2B* in EMCV infection
… 2B. 2B* is translated via programmed ribosomal frameshifting (PRF). Unusually, the PRF is temporally regulated, increasing in efficiency from 0% at early stages of infection to 70% by 6-8 hours post-infection (hpi), thus resulting in time-dependent translation of 2B*. Truncation mutation of 2B* …
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The mutation spectra of frameshift reversion ofhis4-38 in REV1 andrev1-1 strains of Saccharomyces cerevisiae
… spontaneous His$\sp+$ revertants of the +1 frameshift mutation his4-38 in Saccharomyces cerevisiae strains XY729 (REV1) and XY760 (rev1-1). To accomplish this, two methods were employed, the double-strand repair of gapped plasmids, and the polymerase chain reaction (PCR). Both approaches …
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The mutation spectra of 4-nitroquinoline N-oxide-induced frameshift reversion ofhis4-38 in REV1 and rev1-1 strains of Saccharomyces cerevisiae
… (4NQO)-induced His$\sp+$ revertants of the +1 frameshift mutation his4-38 in Saccharomyces cerevisiae strains XY729 (REV1) and XY760 (rev1-1). These two strains differ in their ability to repair DNA. The REV1 gene is involved in error-prone repair of DNA. The two strains differed in their …
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Trans-Acting Factors Affecting Retroviral Recoding
… an alteration of the reading frame of the mRNA (frameshift). It has been determined that retroviral readthrough and frameshift require cis-acting factors in the mRNA to stimulate recoding on the eukaryotic ribosome. Here we investigate the affects of trans acting factors on recoding, primarily in …
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The plant-activation of m-phenylenediamine and its mutagenic, biochemical and molecular characterization
… simple aromatic amines are activated into potent frameshift mutagens by cultured tobacco cells (TX1). In this study, biochemical, genetic, and molecular approaches were used to define the mechanisms involved in the plant-activation of an arylamine model compound, m-Phenylenediamine. This study …
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Multi-color visualization and quantification of single RNA translation and HIV-1 programmed ribosomal frameshifting in living cells
… modality was used to investigate ribosomal frameshifting during translation. Frameshifts occur through a ribosomal +1(-2) or -1 (+2) nucleotide(s) "slip" into another frame and are implicated in both human disease and viral infections. While previous work has uncovered many mechanistic …
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Ribosome profiling of porcine reproductive and respiratory syndrome virus reveals novel features of viral gene expression
… PRRSV genome contains two programmed ribosomal frameshift (PRF) signals, at which conserved elements promote the backwards slippage of the ribosome by one or two nucleotides. PRRSV encodes a canonical −1 PRF site, at which frameshifting is directed by a downstream RNA pseudoknot structure, to …
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Mutations in Connexin43 and Connexin40 Associated with Human Atrial Fibrillation; Physiology and Mechanism
… To characterize trafficking of a novel somatic frameshift Cx43 mutant and three novel Cx40 mutants, fluorescently tagged connexins were visualized in HeLa cells using confocal microscopy. Electrophysiological studies were performed by a member of our lab to test the function of mutant gap …
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CELF1, PTBP1, and RBFOX2-mediated alternative splicing regulation in cardiovascular diseases
… damaging de novo RBFOX2 mutations (nonsense, frameshift, and splice site) have been identified. Here we provide evidence that the nonsense and frameshift RBFOX2 mutants are unable to promote proper splicing of their target genes despite normal subcellular localization of these mutants. …
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Toxicity mechanism of anthropogenic water contaminants: drinking water disinfection by-products (haloacetic acids) and particle associated contaminants from sealcoats (polyaromatic hydrocarbons) in lake sediments
… mutations as compared to the induction of frameshift mutations. Soot extracts induced a relatively higher rate of frameshift mutations as compared to the coal tar extract frameshift mutation rate.
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THE ROLE OF DMRT GENES DURING NEUROGENESIS IN THE ZEBRAFISH FOREBRAIN
… to knock-out dmrt3 in zebrafish by introducing frameshift mutations of variable length into the dmrt3 locus using zinc finger nucleases. These dmrt3 mutants provide an important tool for future studies on the effect of a homozygous dmrt3 knock-out on brain development.
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Novel Cas orthologs from natural reservoirs and design of a directed evolution approach in mammalian cells to enhance their editing potential
… that coupling, we built an artificial stop/frameshift cassette flanked by introns and inserted it in the coding sequence of a EGFP reporter. We demonstrated that insertion of the cassette abrogated gene function, and that Cas editing at its splice sites could efficiently alter the splicing …
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Discovery of the novel mouFSnrp gene and the characterisation of its in situ expression profile during mouse neurogenesis
… in the mouse genome. This gene, called mouse frameshift nrp (mouFSnrp), was hypothesised exist as a -1bp frameshift to another predicted gene AlkB. This project involved the identification of the mouFSnrp gene, and the characterisation of its expression pattern and ontogeny during mouse neural …
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Evaluating the Conservation of RNA Packaging Enhancer Elements Across Lentiviruses
… that the region overlapping the Ribosomal Frameshift Signal (RFS) acted to enhance RNA packaging 50 fold and was thus named the genomic RNA packaging enhancer element (GRPE). To determine if the GRPE was conserved across lentiviruses, using the same approach as Chamanian et al., deletions …
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A study of the molecular mechanism of the attenuation response in the threonine operon of Escherichia coli by synthesis of mutant attenuators
… deletion at position 106 (106$\Delta$). The frameshift mutation removes the leader translation stop codon to determine whether unstopped ribosomes translating the distal regulatory region interfere with terminator function. All nine variants, including the wild type control, bear a deletion …
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STRUCTURAL STUDY OF HUMAN CATENIN ΒETA-LIKE PROTEIN 1 AND DOUBLE RNA PSEUDOKNOTS IN -1 RIBOSOMAL FRAMESHIFTING
… in the regulation of -1 programmed ribosomal frameshifting in RNA viruses −1 programmed ribosomal frameshifting (PRF) is utilized by many viruses to synthesize their enzymatic and structural proteins at a defined ratio. For efficient −1 PRF, two cis-acting elements are required--a …
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Studies on the Mislocalized Exonuclease TREX1 that Causes a Systemic Vasculopathy
… of death 53.1 ± 9.6 years). CHARIOT is caused by frameshift mutations in three prime repair exonuclease 1 (TREX1). TREX1, also known as DNase III, is the major mammalian intracellular DNA 3′-5´ exonuclease and the frameshift mutations result in a protein that retains nuclease activity but is …
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Messenger RNA Destabilization by -1 Programmed Ribosomal Frameshifting
… studies have identified programmed -1 ribosomal frameshifting (-1 PRF) signals in eukaryotic genomic sequences, and suggested a role in mRNA stability. This work improves and extends the computational methods used to search for potential -1 PRF signals. It continues to examine four yeast -1 PRF …
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Analisi molecolare in pazienti italiani con sindrome di Lowe
… out of twenty consisted of truncating mutations (frameshift, nonsense, splice site and genomic deletion), and four were missense mutations. The mutations were distributed in the second half of the gene as previously described in other populations. Our results on the Italian population are similar …
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