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Showing 1 to 14 of 14 for “"fosfomycin"”.
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Structural analysis of hydroxypropylphosphonic acid epoxidase : a fosfomycin biosynthetic enzyme
An X-ray crystallographic study of the fosfomycin biosynthetic enzyme hydroxypropylphosphonic acid epoxidase (HppE) from Streptomyces wedmorensis is presented. Structural analysis of this cupin mononuclear iron enzyme in complex with its in vivo substrate, and comparison with apo- and holo-enzyme …
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Identification and Inhibition of Fosfomycin Resistance Enzymes for Structure-Based Drug Design
… therapy to restore the functionality of the drug fosfomycin by using in silico high-throughput virtual screening (HTVS) to identify inhibitors of fosfomycin resistance enzymes.The purpose of this work is to characterize fosfomycin resistance enzymes and identify enzymatic inhibitors to restore the …
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Characterization of antimicrobial resistance among canine urinary isolates in Western Canada
… Seven E. coli and four S. pseudintermedius were fosfomycin resistant. Overall, twelve isolates harbored CMY-2 type AmpC -lactamases, and seven produced CTX-M type extended spectrum -lactamases (ESBLs). Of the fosfomycin resistant E. coli, one also possessed an CMY-2 type AmpC -lactamase. Of …
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Diverse enzyme reactions in phosphonate natural product biosynthesis
… Biosynthesis of the clinically used antibiotic fosfomycin can occur by two convergent routes in Streptomyces and Pseudomonas species. The pathways only share the first and last steps, which are catalyzed by phosphoenolpyruvate mutase and 2-hydroxypropylphosphonate epoxidase (HppE), respectively. …
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Investigations of metalloenzymes involved in bacterial natural product biosynthesis
… biosynthesis of two different natural products: fosfomycin, a clinically used phosphonate antibiotic discovered in 1969, and 3-thiaglutamate, a novel amino acid analog of unknown function discovered in 2019. In Chapter 2, newly available techniques were used to heterologously express and purify …
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Investigations into the molecular basis of enzyme-catalyzed reactions in natural product biosynthesis
… is an enzyme involved in the biosynthesis of fosfomycin – a phosphonate antibacterial used to treat urinary tract infections. This enzyme, termed PsfC, possesses a metallohydrolase fold, but not share the canonical trinuclear metal center-binding motif associated with the class of enzymes. …
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Evaluation of HB&L Automated System for Rapid Diagnosis of Urinary Tract Infections // Оценка на автоматизираната система HB&L за бърза диагностика на инфекции на уринарния тракт
… agents used in outpatient practice was studied: fosfomycin, nitrofurantoin, and 5-nitroxoline. A high concordance of results was demonstrated. The system showed high specificity, sensitivity, and positive and negative predictive values. Based on the results, a working algorithm for the accurate …
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Mechanistic investigations of enzymes in phosphonate metabolism
… implications for the biosynthetic pathway for fosfomycin are discussed. Finally, observations made while studying an aldehyde dehydrogenase that oxidizes PnAA to phosphonoacetate are reported.
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The evolution of AroA and MurA enzymes from Bacillus
… AroA and MurA by their inhibitors glyphosate and fosfomycin respectively remains unchanged for AroA LCA and MurA LCA. On the other hand, the high affinity for PEP and increased glyphosate sensitivity exhibited by AroA LCA indicate AroA LCA to be intermediate between class I (glyphosate sensitive) …
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Studies of unusual catalysis : a tale of four enzymes from diverse biosynthesis pathways
… unactivated carbon center in the biosynthesis of fosfomycin, which is a clinically relevant antibiotic. OxsB is responsible for the biosynthesis of oxetanocin A, an oxetane ring containing nucleoside that exhibits antiviral activity. Efforts have been made to isolate active Fom3 and reconstitute …
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Investigation of the biosynthesis of the phosphonate antibiotics bialaphos and dehydrophos
… was used to methylate the clinical antibiotic fosfomycin and the clinical anti-malaria candidate fosmidomycin, showing its promise for applications in bioengineering. Overall, the findings in this thesis provide valuable insights in Nature’s strategy for making novel phosphonates which can be …
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Untersuchungen zur Induktion und zum Transfer der Vancomycin-Resistenz vom VanA-Typ sowie zur Flavophospholipol-Resistenz in Enterococcus faecium
… leichte Induktion des VanA-Ligase Proteins durch Fosfomycin, Cefalexin und Cefuroxim, Meropenem und Clindamycin nachgewiesen werden. Somit konnte gezeigt werden, dass Cefuroxim und Clindamycin zwei Antibiotika, die in klinischen Studien eine Besiedelung mit VRE begünstigen, auch eine geringe …
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Effect of Standard Post-harvest Interventions of Fresh Vegetables on Bacterial Community Dynamics, Pathogen Survival and Antibiotic Resistance
… aminoglycoside, bacitracin, β-lactam, and fosfomycin. Irradiation resulted in significant reductions (~3.5 log CFU/g) of inoculated antibiotic-resistant E. coli O157:H7 and Pseudomonas sp. on lettuce surfaces (ANOVA, p<0.05). The lettuce resistome, represented by the Bray-Curtis similarity …
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Homeostasis of primary and secondary metabolites
The student, Philip Olivares, submitted this Dissertation for approval on 2019-03-11 at 16:15.