Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"forward programming"”.
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Molecular regulation of BRD3 in forward programming of Megakaryocytes
… were capable to differentiate into MKs, using a forward programming protocol, demonstrating that BRD3 is not essential for MK differentiation. I found that a subset of genes was differentially expressed in the absence of BRD3, despite genome-wide chromatin accessibility and H3K27ac signatures …
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Forward programming of human pluripotent stem cells to a megakaryocyte-erythrocyte bi-potent progenitor population: an in vitro system for the production of platelets and red blood cells for transfusion medicine.
… almost any cell type. Using a technique called forward programming, we over express three key haematological transcription factors (TFs), pushing iPSCs towards the megakaryocyte lineage, to produce mature megakaryocytes, the platelet precursor cell type. A major limitation of the forward …
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The role of Synaptic Genes NRXN1 and SHANK3 in Autism: an induced pluripotent stem cell study
… of this thesis is that iNeurons derived by NGN2 forward programming approach would express autism related genes, post synaptic density molecules, synaptic receptors and demonstrate mature electrophysiological activity relevant for studying autism related functional phenotypes, within four weeks …
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Cellular Phenotypes Associated with NRXN1 Mutations in Autism; an iPSC Study
… thesis lie in the technological advancements of forward programming and iPSC technologies, however further experiments with a bigger sample size might be required to validate and support the findings described. Thus, these data contribute to our overall understanding of how NRXN1 may contribute …
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Cytokine receptor-like factor 3 (CRLF3): A novel regulator of platelet biogenesis and potential drug target for thrombocythaemia
… megakaryocytes from these TAP-tagged iPSCs by forward programming. However, as yet we have not been able to generate enough MKs to have adequate material to perform immunoprecipitation assays. Therefore, the interactome of CRLF3 in MKs remains unknown. In conclusion, we identified a mechanism …
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Genetic and functional studies in inherited platelet disorders
… (Met2080Lys or Pro2100Leu) in homozygosity. Forward programming of iPSCs to MKs enabled comparison of the morphology and α-granule protein expression between NBEAL2 variant MKs and wild type. The cellular endophenotype of these cell models was explored through RNA-sequencing and MS proteomics …