Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"forward genetics"”.
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Identifying genes related to symbiotic nitrogen fixation in Medicago truncatula Tnt1 insertion mutants using a forward genetics approach
Nitrogen is an essential nutrient required by all plants. Although the earth’s atmosphere consists of approximately 78% nitrogen, it exists in an unusable form for plants. Current agricultural practices rely on industrial nitrogen fixation to produce fertilizers that improve crop productivity. …
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Expanding Genetic and Genomic Resources for Sex Separation and Mosquito Control Strategies
… bringing together elements of classical genetics, recent advances in sequencing and genome-editing technologies, and the use of modern forward genetics approaches. Chapter 2 of this dissertation explores the use of the Oxford Nanopore Sequencing Technology for the first time in …
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Identifying Modulators of the Development of Acute Functional Tolerance to Ethanol in Caenorhabditis elegans.
… their development of addiction. We have taken a forward genetics approach to study one component of initial response, acute functional tolerance (AFT), in Caenorhabditis elegans. We identified bet11, a mutation that causes animals to be defective in the development of AFT. Genetic analysis …
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Invisible to arbuscular mycorrhizal fungi: Positional cloning and characterisation of a novel arbuscular mycorrhizal mutant in Zea mays
… even before physical contact has occurred. A forward genetics screen has identified invisible to arbuscular mycorrhizal fungi (ina), a Zea mays mutant phenotype that displays a complete block in colonisation when inoculated with the arbuscular mycorrhizal fungus Rhizophagus irregularis. …
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The effect of an argumentation-based instruction on grade twelve learners’ understanding of genetics in two schools in the Western Cape
… This study seeks to explore the problems of genetics learning and to identify possible ways forward. Genetics is often thought of as a subject or a topic in Life Sciences (previously called Biology) that is difficult to learn and understand, especially for learners. The study is exploring the …
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An Integrated Software System for EEG/EMG-Based Forward Genetic Screen of Sleep/Wake Abnormalities in ENU-Mutagenized Mice
… but highly interesting question through forward genetics in mice. We have initiated a dominant screen of ENU-mutagenized mice, in which a comprehensive set of sleep parameters is measured via EEG/EMG-based somnography analysis in basal light/dark periods as well as in the recovery period …
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Cell Fate Specification and the Regulation of RNA-dependent DNA Methylation in the Arabidopsis Root Meristem
… (EMS) mutagenized marker line to perform a forward genetics screen. The goal of this screen was to identify novel genes involved in the specification and differentiation of the cortex tissue. Mapping analysis from the results obtained in this screen revealed a new allele of BRASSINOSTEROID4 …
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Dissection of Drought Responses in Arabidopsis
… late stage of moderate drought. Taking a reverse genetics approach, a MYB transcription factor (MYB109) has been identified as a regulator of growth under drought and salt stress. Global expression profiling showed possible mechanisms of how MYB109 modulates growth under drought conditions: as a …
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Investigation of Rare Genetic Variation in Autism Spectrum Disorder by Genomic Screens
… size limitations of human studies, I performed a forward genetics screen in mice to identify mutations associated with ASD-like behaviors (Chapter 4). I identified mutations linked to altered vocalization and altered repetitive and social behavior phenotypes in mice. To show the synergy of the …
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Investigation into Tissue-Specific Mechanisms of Mitochondrial Dysfunction: Models of SUCLA2 Deficiency and a Screen for Potential Genetic Modifiers
… using two genetic approaches. First, reverse genetics tools are used to generate tissue-specific mouse models of succinyl-CoA synthetase deficiency, which is a known cause of mitochondrial disease in humans. In parallel, forward genetics is used to screen for variation in mitochondrial …
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DNA Damage responses in plant stem cells
… thesis. Here, a candidate gene approach and a forward genetics screen in the root stem cells did not yield new factors of the pathway leading to PCD in root stem cells. However, a specific ecotype showed an absence of DSBs-induced PCD, revealing natural variation in stem cell responses to DSBs. …
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Identification And Characterization Of Mediators Of Toxicity Of Aβ Oligomers By Genome Wide Screening In <i>Caenorhabditis Elegans</i>
… genes involved in the Aβ-dependent paralysis (forward genetics). This analysis allowed identification of a mutated clone showing a complete lack of paralysis, despite this it not bear mutations in the Aβ coding region, and accumulates Aβ transcript and protein levels comparable to that of the …
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Cellular and Circuit Consequences of a Genetic Locus for Attention
… I aimed to provide a new perspective by taking a forward genetics approach to identify genes with prominent contributions to attentional performance. We studied 200 mice from a highly genetically diverse, multi parent mouse population on measures of pre-attentive processing and through genetic …
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Genetic studies of phenotypic variants in the woodland strawberry, (Fragaria vesca)
… plants. This thesis represents some of the first forward genetics studies evaluating putative T-DNA insertional mutants in F. vesca. The observed phenotypes include alterations to floral development, anthocyanin pigmentation and leaf structure. The floral development mutant named green petal (gp) …
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Identification of novel components of the zinc homeostasis mechanism in Arabidopsis thaliana
… and aid the biofortification research, a forward genetics approach was adopted to identify molecular components of the Zn homeostasis network in plants. Previous observations have indicated that irregularity in zinc homeostasis mechanism often leads to a reduction in zinc tolerance of …
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Using Genetic Diversity to Understand Susceptibility to Cognitive Decline in Aging and Alzheimer’s Disease
… specific genes in human studies include complex genetics, difficulty in controlling environmental factors, and limited access to human brain tissue. Here, we turned to genetically diverse mice from the BXD genetic reference panel (GRP) to overcome some of the barriers traditionally associated …
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Developing molecular genetic tools for gene discovery in soybean (Glycine max)
… fast neutron-induced phenotypes. Through forward genetics, we identified a deleted DNA fragment in chromosome 17 that co-segregated with the production of big seeds and leaves in the fast neutron mutant line K83. CRISPR/Cas9 gene editing system was employed to unveil the function of GmKIX1 …
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Understanding the molecular basis of Zymoseptoria tritici-wheat interactions
… to develop molecular tools to facilitate forward and reverse genetic screens in Z.tritici for characterizing putative effector genes involved in STB disease. As a part of this study, a genome editing tool, CRISPR/Cas9, was employed to facilitate high frequency targeted genome manipulations …
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Understanding the molecular basis of Zymoseptoria tritici-wheat interactions
… to develop molecular tools to facilitate forward and reverse genetic screens in Z.tritici for characterizing putative effector genes involved in STB disease. As a part of this study, a genome editing tool, CRISPR/Cas9, was employed to facilitate high frequency targeted genome manipulations …
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Transposon Tagging in Strawberry and Potato and Characterization of Representative Strawberry Mutants
Strawberry and potato are both important crop species in the world providing various nutritional values. The cultivated strawberry, Fragaria ananassa, is a fruit crop with a complex genome (2n=8x=56) whereas the diploid woodland strawberry, Fragaria vesca, has a smaller genome (2n=2x=14, 240 Mb) …
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