Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 40 for “"forward genetic screen"”.
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A forward genetic screen to identify factors that control meiotic recombination in Arabidopsis thaliana
Meiotic recombination promotes genetic variation by reciprocal exchange of genetic material producing novel allelic combinations that influence important agronomic traits in crop plants. Therefore, harnessing meiotic recombination has the potential to accelerate crop improvement via classical …
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An Integrated Software System for EEG/EMG-Based Forward Genetic Screen of Sleep/Wake Abnormalities in ENU-Mutagenized Mice
… but highly interesting question through forward genetics in mice. We have initiated a dominant screen of ENU-mutagenized mice, in which a comprehensive set of sleep parameters is measured via EEG/EMG-based somnography analysis in basal light/dark periods as well as in the recovery period …
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A Forward Genetic Screen Revealed the Role of POLDIP2 in Ensuring Maternal Inheritance of Mitochondrial DNA in Drosophila melanogaster
… homeostasis and apoptosis. They house their own genetic materials known as mitochondrial DNA (mtDNA). In most animals, the inheritance of mtDNA follows a uniparental pattern, generally from the maternal side. Maternal inheritance is accomplished by mechanisms actively degrading paternal mtDNA …
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N-Glycosylation Protects Host from Mycobacterial Infection
… and restrict their growth. Through a zebrafish forward genetic screen, we have identified the early steps of N-glycosylation are required for the macrophage to resist mycobacterial infections. N-glycosylation attaches an oligosaccharide molecule to nascent secretory or membrane proteins within …
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The Characterization of Nemadipine and Migrazole as Small Molecule Tools for Use in the Nematode Caenorhabditis elegans
… investigation. They provide an alternative to genetic perturbation and may offer more control over a target’s activity. C. elegans has recently gained prominence as a platform to discover new chemical tools. Through large-scale screens for compounds that induce phenotypes consistent with the …
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Forward screening for herbicide resistance in Arabidopsis thaliana and the monocot species Triticum aestivum
… thaliana mutants were identified from previous forward genetic screens. Some identified CBI resistant mutants demonstrated altered cellulose crystallinity, and cell wall saccharification. In a larger crop species, these characteristics would allow for plant cell wall material to be efficiently …
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Mosaic Analysis of Neurogenesis and Neuronal Morphogenesis in Drosophila Learning and Memory Center Mushroom Bodies
Based on the above findings, a forward genetic screen using MARCM (m&barbelow;osaic a&barbelow;nalysis with r&barbelow;epressible c&barbelow;ell m&barbelow;arker) system, which uniquely labels wild type or homozygous mutant single-cell or subcell lineages born at specific developmental stages, was …
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Zebrafish Mutagenesis Study of Host Determinants of Mycobacterial Infection Outcome
This thesis describes a large-scale forward genetic screen in zebrafish for host determinants of mycobacterial infection outcome that I participated in. Zebrafish larvae from families randomly mutagenized with N-ethyl-N-nitrosourea were infected with Mycobacterium marinum and monitored over time. …
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The Roles of daf-6 and Cell-Cell Interactions in Sensory Organ Morphogenesis
… of cell shape, position, and orientation. The genetic regulation of these morphogenetic processes is poorly understood. As a model for organ morphogenesis, I studied the development of the Caenorhabditis elegans amphid sensory organ. Sensory organs in diverse species are often composed of …
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Molecular Physiology of the Chlamydomonas Pyrenoid
… spectrometry. In a complementary approach, a forward genetic screen based on high-throughput immunofluorescence localization of Rubisco aimed to identify key pyrenoid assembly factors. The present study combined and developed a wide range of molecular, physiological and computational …
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Genetic analysis of endoplasmic reticulum homeostasis during stress and infection of Caenorhabditis elegans
… ER homeostasis in C. elegans, we conducted a forward genetic screen and identified suppressors of xbp-1 mutant larval lethality on P. aeruginosa. In this work, I outline the characterization of several identified mutations that each affect a gene encoding a broadly conserved transcriptional …
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Developing a Caenorhabditis elegans Model for Marfan Syndrome
… growth factor beta) and BMP (bone morphogenetic protein) within the extracellular matrix (ECM). The primary objective of this study was to establish a C.elegans MFS model and use this model to determine which genes interact with a C.elegans fbn1 homolog, MUA-3 and ascertain the role of …
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Forward genetic analysis of cellulose biosynthesis inhibitor resistance and wall hydrolysis sensitivity.
… assembly and structure in plants. We conducted screens using the herbicides, isoxaben and flupoxam which inhibit cellulose biosynthesis in higher plants. Mutations resulting in a high degree of resistance to isoxaben (ixr) or flupoxam (fxr) were attributed to single amino acid substitutions in …
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The identification and characterization of novel persistence genes in chlamydia trachomatis
… from persistence remain largely unknown. Using a forward genetic screen, we identified six Susceptible to IFN-γ mediated Persistence (Sip) mutants that have diminished capacities to reactivate from persistence with indole. Mapping the deleterious persistence alleles in three of the Sip mutants …
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A Mutation in Alk6b Causes Impaired Germ Cell Differentation and Testicular Germ Cell Tumors in Zebrafish
… line with a high incidence of GCT during a forward genetic screen to identify cancer susceptibility loci. Homozygous adult males develop tumors consisting of undifferentiated spermatogonia by 4 months of age while heterozygous males develop tumors around 7 to 9 months of age. We used …
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Components Required for the Death and Degradation of the Linker Cell in C. Elegans
… not currently known. Using a protein interaction screen and classical genetic studies, I propose that the homeodomain protein kinase HPK-1 is required to activate the heat shock function of HSF-1, thereby indirectly inhibiting its cell death role. I hypothesize that PQN-41C, a polyglutamine …
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SYSTEMIC AND TRANSGENERATIONAL REGULATION OF GENE EXPRESSION BY SMALL RNAS IN C. ELEGANS
… same target sequence expressed from different genetic contexts shows varying susceptibility to transgenerational silencing. Most tested genes recover from silencing in a few generations suggestive of mechanisms that repair changes induced in ancestors. We characterize a unique gene that is …
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Genetic and Cytological Analysis of the Spindle Assembly Checkpoint Component BUB-3 in DNA Damage Response
… in multicellular organisms. We performed a forward genetic screen in Caenorhabditis elegans to uncover new genes involved in the repair of DNA damage induced by ionizing radiation. We isolated a mutation named gt2000, which confers hypersensitivity to ionizing radiation. We showed that …
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V-ATPase regulation of Hypoxia Inducible transcription Factors
… in regulating HIF-$\alpha$ turnover. Unbiased forward genetic screens in near-haploid human cells identified that disruption of the V-ATPase leads to activation of HIFs in aerobic conditions. Rather than preventing the lysosomal degradation of HIF-$\alpha$, I found that V-ATPase inhibition …
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Characterisation of new regulators and neural plasticity of the O2-sensing circuit in Caenorhabditis elegans
… in the sensory neuron ADL. Here, we perform a forward genetic screen to isolate mutants that confer O2-escape behaviour on N2 animals. Using whole-genome sequencing and genetic mapping, we identify several conserved genes not previously shown to regulate aggregation. Among these, we …
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