Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"formins"”.
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Biochemical and Microscopic Characterization of INFT-1: an Inverted Formin in C. elegans
Formins are potent regulators of actin dynamics that can remodel the actin cytoskeleton to control cell shape, cell cytokinesis, and cell morphogenesis. The defining feature of formins is the formin homology 2 (FH2) domain (Paul and Pollard, 2008), which promotes actin filament assembly while …
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ROLE OF FORMIN FHOD1 IN THE ACTIN PEDESTAL FORMATION
… In this work, we explored the role of formins in the actin pedestal formation. We discovered that the formin, FHOD1 localized to the pedestal base and its knockdown drastically reduced pedestal surface area. FHOD1 knockdowns also compromised host responses to EPEC. Interestingly, …
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INTERRELATIONSHIP BETWEEN CONTRACTILITY AND DYNAMICS OF ACTOMYOSIN SYSTEM IN NON-MUSCLE CELLS
… the force exertion. I hence proposed that formins could physically link actin filaments with each other, thus participating in force transmission processes. On the other hand, drug inhibition of myosin prevented actin incorporation along VSFs. Furthermore, stretching rescued inhibited actin …
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Determining Molecular Mechanisms of Cell Division in Fission Yeast by Testing Major Assumptions of the Search, Capture, Pull, and Release Model of Contractile-Ring Assembly
… during cytokinesis. The fission yeast has three formins that nucleate actin filaments. Only Cdc12 is essential for contractile-ring assembly, but For3 nucleates about 10% of the actin filaments at the division site. Two temperature-sensitive alleles of <i>cdc12</i>, <i>cdc12-112</i> and …
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Self-assembly of filopodia-like structures on supported lipid bilayers
… which may involve multiple factors including formins. We propose a model for filopodia formation involving an initial clustering of Arp 2/3 complex regulators, self-assembly of filopodial tip complexes on the membrane, resulting in the outgrowth of parallel actin bundles.
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Modelling and image processing of microtubule dynamics and organisation
… highlyaligned microtubules, and inhibition of formins rescued this phenotype. Inhibition of Rac1 produced less-aligned microtubules in otherwise unperturbed cells, while in EB2-depleted cells, microtubules were further aligned. The method was also used to quantify alignment in plant microtubule …
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Toca-1 driven actin polymerisation at membranes
… implicated in filopodia formation, such as formins. I found that extracts depleted of Toca-1 binding partners no longer forms filopodia-like structures and subsequently optimised pull-down assays to identify Toca-1 binding partners by mass-spectrometry. I identified four formins, Diaph1, …
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MOLECULAR MECHANISMS OF ACTIN FILAMENT ASSEMBLY AND TURNOVER
… adapted for filament severing and why other formins are not. At the pointed end, it was typically thought that only capping or depolymerization can occur in the cell. However, three structures of muscle leiomodin-2 (Lmod2) inform a mechanism of pointed-end elongation. Lmod2 is observed …
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Rho GTPases and their regulators in cell polarity of the filamentous ascomycete Neurospora crassa
… dass die Aktivität der Proteinkinase C und des Formins BNI1 nur von RHO1, nicht aber von RHO2, kontrolliert wird. Parallel stützen Untersuchungen zur subzellulären Lokalisation von NCU00668, RHO1 und RHO2 die Annahme, dass die Komponenten dieses GTPase Moduls sowohl gemeinsame als auch …
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Calcium and actin coordinate egg activation and the metaphase-anaphase transition in Drosophila oocytes
… population? I first highlight the requirement of Formins in production of this population. I further reveal that the spindle-like actin is required for regulating the formation and morphology of the spindle and therefore the accurate movement of chromosomes during meiosis, demonstrating remarkable …
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Actin Cable Function and Regulation in the Budding Yeast, Saccharomyces cerevisiae
… Previous studies revealed a role for the yeast formins (Bni1p and Bnr1p) in stimulating polymerization of F-actin for actin cable formation, elongation and retrograde flow, and for other actin cable constituents including tropomyosins and actin bundling proteins in stabilizing and organizing …
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Characterisation of the Rho GTPase target Dishevelled Associated Activator of Morphogenesis 1 (DAAM1)
Dishevelled Associated Activator of Morphogenesis 1 (DAAM1) is a formin which is important for gastrulation. In this thesis, we have sought to characterise DAAM1 in terms of its regulation and function. DAAM1 was found to localise to the perinuclear/Golgi region as well as to the actin/myosin II …
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Regulation of Inverted Formin-1 (INF1) by Microtubule-Affinity Regulating Kinase 2 (MARK2)
The actin and microtubule cytoskeleton plays a critical role in the establishment of cell polarity. Cell processes like mitosis and migration rely on the reorganization of the cytoskeleton to properly function. One driver of cell polarity is the formin, Inverted Formin-1 (INF1). INF1 is able to …
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Cytotoxic T lymphocyte biology under the microscope
… and contractility, namely the Arp2/3 complex, formins, and nonmuscle myosin IIa, is determined. Finally, I turn to forces at the cytotoxic immune synapse by creating artificial synapses on traction force gels. This reveals dynamic changes in actin conformation here, which correlate once again …