Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"formin"”.
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ROLE OF FORMIN FHOD1 IN THE ACTIN PEDESTAL FORMATION
… In this work, we explored the role of formins in the actin pedestal formation. We discovered that the formin, FHOD1 localized to the pedestal base and its knockdown drastically reduced pedestal surface area. FHOD1 knockdowns also compromised host responses to EPEC. Interestingly, …
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SINGLE-MOLECULE STUDY OF THE FORCE-INDUCED ACTIN POLYMERIZATION MEDIATED BY FORMIN
… TO PROMOTE ACTIN POLYMERIZATION VIA BARBED-END FORMIN PROTEINS AS A NOVEL MECHANISM OF MECHANOTRANSDUCTION. HOWEVER, THIS HYPOTHESIS HAS NOT BEEN WELL CONCLUDED DUE TO THE LACK OF SENSITIVE APPROACH. MY PHD STUDIES, THEREFORE, AIMS TO ESTABLISH EFFECTIVE METHODS BASED ON THREE SINGLE MOLECULE …
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Regulation of Inverted Formin-1 (INF1) by Microtubule-Affinity Regulating Kinase 2 (MARK2)
… function. One driver of cell polarity is the formin, Inverted Formin-1 (INF1). INF1 is able to induce F-actin formation, activate the Serum Response Factor (SRF) pathway, stabilize microtubules, associate with microtubules, and disperse the Golgi body. Regulation of INF1 is unique, since it …
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Biochemical and Microscopic Characterization of INFT-1: an Inverted Formin in C. elegans
Formins are potent regulators of actin dynamics that can remodel the actin cytoskeleton to control cell shape, cell cytokinesis, and cell morphogenesis. The defining feature of formins is the formin homology 2 (FH2) domain (Paul and Pollard, 2008), which promotes actin filament assembly while …
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NOVEL MECHANISMS IN THE REGULATION OF PERINUCLEAR ACTIN ASSEMBLY
… dynamics and organization. Inside the cell, the formin protein family constitutes an important group of actin regulators. However, how these external and internal regulators control actin dynamics in the perinuclear area has not been sufficiently studied. Work in this thesis concentrates on …
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MOLECULAR MECHANISMS OF ACTIN FILAMENT ASSEMBLY AND TURNOVER
… classes of ABPs are now resolved. Structures of formin at the fast-growing barbed end, as well as formin with incoming profilin actin reveal a step-by-step mechanism of actin polymerization. In addition, we show how inverted formin-2 (INF2) is uniquely adapted for filament severing and why other …
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Self-assembly of filopodia-like structures on supported lipid bilayers
… tip components such as Diaphanous-related formin and VASP localize to the membrane base of the structures, and bundling protein fascin to the shaft. Actin subunits assemble at the tip and translocate into the shaft. FLS assembly requires negativelycharged lipid membranes, with specific …
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Determining Molecular Mechanisms of Cell Division in Fission Yeast by Testing Major Assumptions of the Search, Capture, Pull, and Release Model of Contractile-Ring Assembly
… the contractile ring of actin, myosin-II, formin, and other proteins, which constricts to divide the cells. We tested some of the basic assumptions of the search, capture, pull, and release (SCPR) model to explain the mechanism of contractile-ring assembly during cytokinesis in fission …
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An analysis of the role of microtubules during tubulogenesis in Drosophila
… and apical medial actin. Further to Shot, the formin Diaphanous regulates both actin and microtubules in morphogenesis, and was also found to localize to MT tips at the apical surface. Overexpression of constitutively active Diaphanous results in precocious invagination of the salivary gland. …
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Characterisation of the Rho GTPase target Dishevelled Associated Activator of Morphogenesis 1 (DAAM1)
… Activator of Morphogenesis 1 (DAAM1) is a formin which is important for gastrulation. In this thesis, we have sought to characterise DAAM1 in terms of its regulation and function. DAAM1 was found to localise to the perinuclear/Golgi region as well as to the actin/myosin II stress fibres. …
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INTERRELATIONSHIP BETWEEN CONTRACTILITY AND DYNAMICS OF ACTOMYOSIN SYSTEM IN NON-MUSCLE CELLS
… the force exertion. I hence proposed that formins could physically link actin filaments with each other, thus participating in force transmission processes. On the other hand, drug inhibition of myosin prevented actin incorporation along VSFs. Furthermore, stretching rescued inhibited actin …
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The Role of Daam1 In Kidney Development
… examine the role of the Wnt/Planar Cell Polarity formin, Dishevelled-associated activator of morphogenesis 1 (Daam1), in shaping nephric tubules. We show that Daam1 governs nephron morphology by regulating E-cadherin-based cell-cell adhesion of progenitor cells and junctional actin filaments' …
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The Role of Daam1 In Kidney Development
… examine the role of the Wnt/Planar Cell Polarity formin, Dishevelled-associated activator of morphogenesis 1 (Daam1), in shaping nephric tubules. We show that Daam1 governs nephron morphology by regulating E-cadherin-based cell-cell adhesion of progenitor cells and junctional actin filaments' …
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Convex lens induced confinement : a novel study of weak and slow DNA & protein interactions
… interaction between actin filaments and formin. CLIC is used to generate data of the binding of a HMG protein to double-stranded DNA with a sufficient signal-to-background over long enough times for efficient particle tracking. The protein-DNA complex's diffusion coefficient is also …
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Actin Cable Function and Regulation in the Budding Yeast, Saccharomyces cerevisiae
… Previous studies revealed a role for the yeast formins (Bni1p and Bnr1p) in stimulating polymerization of F-actin for actin cable formation, elongation and retrograde flow, and for other actin cable constituents including tropomyosins and actin bundling proteins in stabilizing and organizing …
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A Molecular Basis for Divergent, Unstable Actin Filaments in Toxoplasma gondii
… unstable filaments on its own, it is capable of forming longer, conventional filaments when stabilized. Molecular docking was used to identify divergent residues within apicomplexan actin that may impart filament stability and revealed critical substitutions in the hydrophobic plug and …
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Functional Genomic Approaches to Study Cell Polarity Regulation by G1 Cyclins in Saccharomyces cerevisiae
… with G1 cyclins was BNI1, which encodes a yeast formin protein. Overexpression of BNI1 caused an Synthetic Dosage Lethal interaction in the absence of both G1 cyclin pairs while its deletion caused synthetic lethality specifically in the absence of PCL1, PCL2. Consistent with these genetic …
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Characterization of Dishevelled Associated Activator of Morphogenesis 2 (Daam2) in Wnt Signaling During Early Embryonic Development
… to understand its role during embryogenesis. The Formin-homology protein Dishevelled associated activator of morphogenesis 1 (Daam1) was previously characterized as an essential component for non-canonical Wnt-dependent regulation of cytoskeletal reorganization and cell migration. Daam1 and Daam2 …
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MECHANOPROPERTIES, HETEROGENEITY AND CELL MIGRATION IN GLIOBLASTOMA
… The mechano-chemical-induced expression of the formin FMN1 supports the mechanical cohesion of the cytoskeleton and enhances cell’s mechanoproperties, leading to a higher motility and invasive phenotype. In order to scale up the motility screen to several GBM clones, I co-developed SP2G …
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In vitro and in vivo protein folding under stress
… In chapter 2 we mutated the FBP 28 WW domain (formin-binding protein; Leu26 by Asp26 or Trp26) to alter the folding scenario from three-state folding toward two-state or downhill folding at temperatures below the melting point of the protein. The investigation was conducted using a combination …
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