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Showing 1 to 20 of 22 for “"formate dehydrogenase"”.
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The role of molybdenum in the formate dehydrogenase of methanobacterium formicicum
… oxidation products of the pterin cofactor in the formate dehydrogenase of Methanobacterium formicicum revealed that the cofactor is a 6-substituted pterin similar to the molybdopterin of xanthine oxidase. In contrast to the molybdopterin from xanthine oxidase the formate dehydrogenase cofactor was …
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A 'HYBRID' APPROACH FOR THE CHARACTERIZATION OF POSSIBLE PHYSIOLOGICAL ROLES OF ARABIDOPSIS THALIANA FORMATE DEHYDROGENASE FDH
Essendo organismi sessili, le piante non possono fuggire, ma devono costantemente far fronte e diversi stress, come per esempio eccessi di luce o la continua esposizione ai microrganismi. L’enzima formato deidrogenasi (FDH) catalizza l'ossidazione del formato (HCOO-) in anidride carbonica (CO2) …
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A biochemical and physiological characterization of coenzyme F420-reducing hydrogenase from Methanobacterium formicicum
… M. formicicum contained a coenzyme F₄₂₀-mediated formate hydrogenlyase system. Formate hydrogenlyase activity was reconstituted with coenzyme F₄₂₀-reducing hydrogenase, coenzyme F₄₂₀-reducing formate dehydrogenase, and coenzyme F₄₂₀, all purified from M. formicicum. The reconstituted system …
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Investigating [NiFe]-hydrogenases in γ-Proteobacteria
… that functions in partnership with a formate dehydrogenase as a formate hydrogenlyase (FHL). This FHL complex generates H2 and CO2 from the disproportionation of formate (CHOO- + H+ ↔ H2 + CO2). In this Thesis, genetic and biochemical characterisation of Pectobacterium atrosepticum …
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Metabolism of Gallic Acid by Eubacterium Oxidoreducens
… Extracts of cells grown on gallate and formate contained formate dehydrogenase (EC 1.2.1.43) and hydrogenase (EC 1.88.99.1), which were both NADP-linked. These results suggest that the oxidation of formate or H$\sb2$ may be indirectly linked to the reduction of phloroglucinol. A …
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Charakterisierung und Optimierung gel-stabilisierter Zwei-Phasen-Systeme zur stereospezifischen Reduktion hydrophober Verbindungen mit der Carbonyl-Reduktase aus Candida parapsilosis
… 2-propanol and an enzyme-coupled mode with the formate dehydrogenase from candida boidinii (FDH). Both reactions were optimized. The biocatalysts could be immobilized successfully in polyvinyl alcohol beads and into a thermosensitive matrix, based on polyacrylamide. Optimization of the reaction …
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Laccase-based Biocatalytic Systems for Recalcitrant Micro-Pollutants Degradation and Energy Generation
… the power generation during CO2 bioconversion to formate catalysed by formate dehydrogenase. The novel enzymatic power generation is shown to be potentially feasible utilizing wastewater as fuel as well as occurring in tandem with driving bioconversion of chemical feedstock from CO2.
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Biochemistry and genetics of the pathway for the anaerobic degradation of aromatic compounds by Eubacterium oxidoreducens
… of pyrogallol to phloroglucinol and on formate for the reduction of phloroglucinol to dihydrophloroglucinol. Transhydroxylase activity involved in the conversion of pyrogallol to phloroglucinol was induced by growth on aromatic substrates. The formate dehydrogenase was located in the …
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Bioindicators of Reductive Dechlorination in a Dehalococcoides ethenogenes-Containing Mixed Culture: Transcriptional Trends With Respect to Substrate Type, Substrate Concentration, and Culture Operation
… DET1545, the H2ase Hup, and a gene annotated as formate dehydrogenase (Fdh), were highly up-regulated within 1 to 12 h after PCE feeding. Transcription profiles over time suggested that genes belonging to similar functional groups were regulated in similar ways. Expression studies in cultures fed …
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Übertragung der Produktion hydrophober Substanzen mit immobilisierten Biokatalysatoren in den technischen Labormaßstab
… in an enzymatic regeneration process by the formate dehydrogenase (FDH) oxidizing simultaneously the co-substrate formic acid to CO2. This choosen biocatalytic process was scaled and implemented into a technical laboratory-scale while considering and viewing more in detail on system-specific …
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Adapting Proteins for Clinical and Industrial Use
… vector of an industrially relevant enzyme, formate dehydrogenase, allows it to be synthesized and secreted by Pichia pastoris and can be an important first step in creating a self-sustaining and environmentally safe method of producing methanol biofuel. Chapter 5 focuses on investigating a …
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Photoelectrochemical tandem cells with enzymes wired to hierarchically-structured electrodes for solar fuel synthesis
… which performed solar-driven CO2 reduction to formate with formate dehydrogenase by coupling to the PSII−dye photoanode. The system achieved a metabolically-inaccessible pathway of light-driven CO2 fixation to formate and demonstrated a precious metal-free model for solar-driven selective CO2 …
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Tailored diketopyrrolopyrrole-based organic dyes in photoelectrochemical cells for solar fuel synthesis
… biohybrid cathode modified with the redox enzyme formate dehydrogenase for CO₂ reduction to formate. The reported system is the first example of a photoelectrochemical cell achieving simultaneous CO₂ reduction and chemical synthesis in the absence of an applied bias. Finally, the synthesis and …
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Identifying Pantoea Genetic Factors Involved in Host Association by Integrating Comparative Genomics with a Dictyostelium discoideum Grazing Resistance Model
… in subsets of resistant strains including formate dehydrogenase-N, which may increase fitness when dealing with phagocytes. P. ananatis virulence factors that differentiate it from P. stewartii were also determined. All 10 P. ananatis strains have the genes, yhcF and yhcA, which are part of …
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Metabolic and molecular responses to interspecies hydrogen transfer between Ruminococcus albus and Wolinella succinogenes
… increased transcriptional abundance, including formate dehydrogenase and genes involved in nitrate reduction. Transcripts for fumarate respiration were much higher than for nitrate respiration. This is the first study to show at the genome and metabolite levels that R. albus and W. succinogenes …
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Photoelectrochemical and Chemoenzymatic Reforming for Sustainable Fuel Production
… a molecular cobalt porphyrin, a Cu-In alloy and formate dehydrogenase enzyme, which produce CO, syngas and formate, respectively. The versatile PEC systems, which can be assembled in either a ‘two-compartment’ or standalone ‘artificial leaf’ configurations achieve 60‒90% oxidation product …
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Photoelectrochemical fuel synthesis using organic semiconductors with (bio)molecular catalysts
… a range of solar fuels from H2 to syngas and formate. Starting from the rational design of organic photovoltaic (OPV) layers, conventional architecture devices based on the archetypal P3HT:fullerene BHJ were developed. The OPV fabrication techniques were subsequently extended to the BHJ …
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Experimentelle Analyse, Modellierung und biochemische Charakterisierung von Ein- und Zweiphasenreaktionen für die technische Biokatalyse
… interest in the fine chemical industry: Formate dehydrogenase from Candida boidinii (FDH) and benzaldehyde lyase from Pseudomonas fluorescens Biovar I (BAL). For the first time a mechanistic kinetic model for both FDH and BAL was developed. The FDH model demonstrated a superior fit to …
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Immobilising and Interfacing Poly(Heptazine Imide) Ionic Carbon Nitride for Photo(electro)catalytic Fuel and Chemical Production
… photoelectrochemical setup wired to a formate dehydrogenase (FDH) enzyme bio-cathode to perform selective CO2-to-formate conversion. Even under 1 sun, the bio-hybrid PEC system could withstand 10 hours of operation. Finally, the use of a [FeFe]-hydrogenase (H2-evolving enzyme) …
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Entwicklung eines Enzym-Modul-Systems zur Synthese und in situ Regeneration von dTDP-aktivierten Desoxyzuckern
… additional in situ regeneration of NADH with the formate-dehydrogenase was possible and hardly decreased the yield of rhamnosyl-sorangioside. The development of the EMS allows as well the fast characterization of glycosyltransferases (substrate promiscuity) by simple exchange of the aglycons or …
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