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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 82 for “"force spectroscopy"”.
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Correlation Force Spectroscopy for Single Molecule Measurements
This thesis addresses development of a new force spectroscopy tool, correlation force spectroscopy (CFS), for the measurement of the mechanical properties of very small volumes of material (molecular to µm³) at kHz-MHz time-scales. CFS is based on atomic force microscopy (AFM) and the principles of …
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Fluorescence-force spectroscopy at the single molecule level
… conditions. Here, we develop a fluorescence-force spectroscopy method to combine single-molecule fluorescence spectroscopy with optical tweezers. Optical tweezers are used to manipulate and observe mechanical properties on the nanometer scale and piconewton force range. However, once the …
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Synthesis and single-molecule force spectroscopy of supramolecular capsules
… assembly have been studied using single-molecule force spectroscopy to evaluate the dynamics of the self-assembly process. The immobilization of the building blocks of the hydrogen-bonded supramolecular capsule introduced by Kobayashi et al. in 2003 was the basis for successful SMFS experiments. …
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Single molecule fluorescence and force spectroscopy of hairpin ribozyme
Submitted by Elizabeth Kent (eckent2@illinois.edu) on 2012-06-07T18:47:11Z No. of bitstreams: 1 2006_nahas.pdf: 5361832 bytes, checksum: 0d81d12a930597d8c5ce651d401a9bd7 (MD5)
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Escape processes far from thermal equilibrium : path integrals and force spectroscopy
… importance. For example, single-molecule force spectroscopy experiments, and a number of other physical systems are governed by thermally activated transitions out of a metastable state under the action of a steadily increasing external force. This allows to observe the chemical …
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Biological Adhesion on Nanopatterned Substrates Studied with Force Spectroscopy and Microinterferometry
… timescales using magnetic tweezers and atomic force microscopy (AFM). The experiments demonstrated that an integrin binding site spacing of 70 nm and more prevents the cooperative formation of early adhesion clusters in initial adhesion. In long-term adhesion studies, after several hours of …
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Novel Development of Super-Resolution Force Spectroscopy and Applications in Biological Systems
… however, has been rare. Super-resolution force spectroscopy (SURFS) was developed based on the integration of these two techniques, and it has unique biophysical applications in studying drug-DNA interactions and ribosomal translocations. I demonstrate that SURFS has a high force …
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Technology assessment and feasibility study of high-throughput single cell force spectroscopy
… the feasibility of a high throughput single cell force spectroscopy using an atomic force microscopy (AFM)-based platform. A conventional AFM set-up employs a single cantilever probe for force measurement by using laser to detect the deflection of the cantilever structure, and usually can only …
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Single molecule force spectroscopy of single stranded DNA binding protein and Rep helicase
… reveals that the protein condenses ssDNA in the force range 0-10pN and that tension can be used to modulate the ssDNA wrapping state of SSB. Measurements of SSB kinetics indicate that SSB-ssDNA complex formation occurs in a two-step process consisting of a diffusion-limited binding step in which …
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Theoretical and computational modeling of peptide-lipid bilayer interaction studied by dynamic force spectroscopy
… the quantitative description of the detachment force distribution, P(F), and the corresponding force dependent detachment rate, k(F), of a peptide from a lipid bilayer, by assuming that peptide detachment from lipid membranes occurs stochastically along a few dominant diffusive pathways. Besides …
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Mechanical Processes of a Single Synthetic Molecular Machine Studied by AFM-based Force Spectroscopy
… biomolecules are able to generate directional forces by rectifying random thermal motions. This allows these molecular machines to perform mechanical tasks such as intracellular cargo transport or muscle contraction in plants and animals. Although some artificial molecular machines have been …
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Development of a State-of-the-Art Atomic Force Microscope for Improved Force Spectroscopy
… the development of a state-of-the-art atomic force microscope (AFM) for improved force spectroscopy. Although the AFM has been used extensively in this field of research, the performance of the instrument has been limited by inefficient operation techniques, incorrect experimental assumptions, …
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Probing static disorder in protein unfolding and chemical reactions by single-molecule force spectroscopy
… of biomolecular reactions under mechanical force, including protein unfolding and disulfide-bond reduction, probed at the single-molecule level. The advent of single-molecule force spectroscopy has allowed the direct measure of force-dependent reaction rates, providing a powerful approach to …
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Each one teaches one: characterizing active forms of proteins by single molecule force spectroscopy
… deriving from the action of mechanical forces in vivo (Chapter 3) and the complexity of conformational equilibria in intrinsically unstructured proteins and amyloid formation (Chapter 4). My experimental work investigated both these examples by using in both cases the single molecule …
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Molecular mechanics of cartilage : quantification of GAG electrostatic interactions via high-resolution force spectroscopy
Intermolecular repulsion forces between negatively charged glycosaminoglycan (CS-GAG) macromolecules are a major determinant of cartilage biomechanical properties. It is thought that the electrostatic component of the total intermolecular interaction is responsible for 50-75% of the equilibrium …
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Quantifying the fouling resistance of Accident-Tolerant Fuel (ATF) cladding coatings with force spectroscopy
… in porous deposits left by CRUD. CRUD has forced many nuclear reactors into temporary shutdown or production downgrades, costing millions of dollars US per reactor. If the CRUD growth factors could be fully understood, they could be controlled, and the CRUD problem could be eliminated …
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Super-Resolution Force Spectroscopy for Revealing Sub-nucleotide Motion in Ribosomal Frameshifting and DNA Methylation
… focuses on applying super-resolution force spectroscopy (SURFS) as a unique tool to resolve frameshifting products and intermediate states, with unprecedented resolution. Two different forms of forces have been studied, centrifugal force and acoustic radiation force. Using centrifugal …
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Mapping the actin and actin binding proteins interactions : from micromechanics to single molecule force spectroscopy
Mechanical forces play an important role in cell morphology, orientation, migration, adhesion and can even induce apoptosis. The eukaryotic cell is equipped with a dynamic frame, known as the cytoskeleton, that provides the cell's structural integrity in order to sustain and react to such forces. …
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