Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 80 for “"footprinting"”.
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Chemical Footprinting of Polymeric Structure of hnRNPA2 Low Complexity Domain
… amyloid-like fibers. I developed a chemical footprinting method to probe solvent accessible residues in the low complexity domain polymers. By acetylating protein side chains with N-acetylimidazole, and comparing the acetylation in native and denatured conformation by use of SILAC mass …
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Genome-wide Footprinting Uncovers Epigenetic Regulatory Paradigms by Revealing the Chromatin Occupancy Landscape
<p><p>Eukaryotic genomes have extensive flexibility and plasticity to modify transcription and replication programs, yielding a myriad of differentiated cell types and survival mechanisms to adverse environmental conditions. As these genomic processes require precise localization of DNA-binding …
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Life Cycle Assessment of Sustainable Road Pavements: Carbon Footprinting and Multi-attribute Analysis
Sustainability is increasingly becoming a significant part of strategic asset management worldwide. Road agencies are providing guidelines to assess the relative sustainability of road projects. Unfortunately, environmental features of a road project are still considered as stand-alone evaluations, …
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Instructional footprinting: a basis for exploiting concurrency through instructional decomposition and code motion
… of such an approach, we apply instructional footprinting to programs using the Linda coordination language. <i>Linda Primitive Transposition</i> (LPT) and <i>Instruction Piggybacking</i> are discussed as techniques to increase the size of instructional footprints, and thereby improve the …
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A triage approach to streamline environmental footprinting : a case study for liquid crystal displays
Quantitative environmental performance evaluation methods are desired given the growing certification and labeling landscape for consumer goods. Challenges associated with existing methods, such as life cycle assessment (LCA), may be prohibitive for complex goods such as information technology …
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Mobile Carbon Footprinting: Sensing and Shaping the Carbon Emissions of Daily Activities Using Digital Technologies
… digital feedback tool, named Mobile Carbon Footprinting, which uses daily activity diaries to track and compare emissions across an interrelated set of everyday activities: travel, time at home and away from home, food, and other expenditures. The results of the experimental intervention …
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The Advancement of Mass Spectrometry-based Hydroxyl Radical Protein Footprinting: Application of Novel Analysis Methods to Model Proteins and Apolipoprotein E
… or by folding. The advantage of this protein footprinting method is that it utilizes the reactivity of hydroxyl radicals to stably modify solvent accessible residues non-specifically in a microsecond. The extent of *OH labeling at sites assays their solvent accessibility. We have corroborated …
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Purification and characterization of a poly(dG).poly(dC)-binding protein from Parenchinus angulosis
… slice, by Southwestern blotting and by DNase I footprinting. Ultraviolet crosslinking of the nuclear extract and purified suGF1 revealed the presence of the same specific bands on SDS-P AGE. Optimal suGF1 DNA-binding was shown to occur at relatively high ionic strength (175 mM). suGF1 …
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Footprint Analysis of the Transcriptional Control of Glycogen Phosphorylase 2 in Dictyostelium Discoideum
… characteristic of Dictyostelium promoters, footprinting conditions required intensive optimization with respect to template, nonspecific competitor, source of protein extract and DNase I digestion. Using an endlabeled fragment containing seven repeated sequences (3 TA boxes [TAATTATA], 2 TAG …
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Characterization of Transcriptional Components in Methanocaldococcus Jannaschii: An Integrative Study of Archaeal Basal Transcription Factors and Putative Cis Elements
… regulatory transcription, we used phylogenetic footprinting to investigate the putative cis elements in M. jannaschii. Regions upstream of orthologous genes from four related methanococcal genomes were collected and analyzed for conserved motifs. This method was applied to identify putative rRNA …
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Control of Regulatory Element Function by Histone H3.3
… accessibility and transcription factor (TF) footprinting at promoters. Further, H3.3 KO ESCs show reduced promoter enrichment of p300- a transcriptional coactivator responsible for H3 acetylation at lysine 27 (H3K27ac). Consequently, H3.3 KO ESCs show reduced H3K27ac at promoters, along with …
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Translocation and proteolysis by the energy-dependent protease ClpAP : coordination of conformational changes and active site chemistry
… protein substrates. Synchrotron hydroxyl radical footprinting is used to show that the ClpA D2 loop, located in the pore of ClpA, is in an "up" conformation when ATP is bound and assumes a "down" conformation when ADP is bound. These results provide the first direct experimental support for a …
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Charakterisierung von HSS-2, einer B-zellspezifischen, regulatorischen DNA-Sequenz im Bereich des Ig(lambda)-Enhancers des Menschen
… wurden transiente Transfektionen und In-vivo-Footprinting-Versuche durchgeführt. Die Transfektionsexperimente erfolgten mit den reifen B-Zelllinien MN60 und Daudi. Dazu wurden Luciferasereportergenkonstrukte mit Punktmutationen in den beiden NFkB-Bindungsstellen von HSS-2 oder mit 5’- bzw. …
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Genetic and Biochemical Studies of YqjH and YqjI: Cross-talk between Iron Homeostasis and Nickel Homeostasis in Escherichia Coli
… the protocol for fluorescence-labeled DNase I footprinting, we improved the data quality and reproducibility, which allows it to be used for the quantification of protein-DNA binding affinity. We then used this technique to characterize the mechanism of YqjI regulation in the yqjH-yqjI …
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DNA Triplexes in chemistry biology and medicine
… as follows: MeP > MeC > MePOMe > MeCAE. DNase I footprinting studies indicate that at pH 7.5, MeP, when incorporated into the TFO, enhances the stability of the triplex by three-fold relative to MeC. In addition, UV melting, DNase I footprinting, and gel electrophoresis studies were carried out …
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Identificación y caracterización de elementos regulatorios distales controlados por TGFβ
… de la señalización por TGFβ. Los análisis de footprinting genómico sugieren que los factores de transcripción de la familia AP-1 y SMAD desempeñan un papel principal en este proceso. Por otro lado, la mayoría de los genes regulados por TGFβ se localizan alrededor de los enhancers regulados en …
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The Impact of Selected Gene Mutations to the Activation and DNA Binding Activity of the Transcription Factor VraR
… S164A binding activity to the PvraSR by DNase-I footprinting. Finally, in vivo studies on the effects of substitutions of the above residues on the mRNA level of a number of VraSR regulon genes (fmtA, pbp2, and sgtb) provide insights as to how substitutions at certain positions may be beneficial …
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Transcriptional regulation of basal and phenobarbital-mediated expression of rat cytochrome 2B genes
… was detected in these regions by DNase I footprinting assay. Instead, a region (FP1) from $-$64 to $-$45 was protected by liver nuclear extracts. Mutation of either the BTE or FP1 sequences of CYP2B1 reduced transcriptional activity in HepG2 cells. FP1 was identified as a functional …
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The role of steroidogenic factor-1 (SF-1) in gonadotropin-releasing hormone (GnRH) receptor gene regulation
… αT3-1 and αT4, by gel retardation assays. DNasel footprinting reveals that the major DNA-binding activity in αT3-1 cells on the GnRHR promoter occurs at the SF-1-like site. The SF-1-like sequence specificity of the interaction is demonstrated by gel redardation and DNasel footprinting assays using …
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