Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"food vacuole"”.
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The feeding apparatus and the food vacuole in paramecium multimicronucleatum
Thesis (M.S.)--Michigan State College of Agriculture and Applied Science. Dept. of Zoology, 1951
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Biochemical Characterization of Two Aminopeptidases Involved in Hemoglobin Catabolism in the Food Vacuole of Plasmodium falciparum
… contents to its acidic organelle, known as the food vacuole, where a series of proteases degrade a majority of the host hemoglobin. Two metalloaminopeptidases, PfAPP and PfA-M1, have been previously localized to the food vacuole (in addition to distinct secondary locations for each), implicating …
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Investigations into the Nature of the Endosomal System in Plasmodium falciparum
… of a specialized acidic organelle, known as the food vacuole, where the parasite catabolizes the majority of its host cell hemoglobin. We analyzed the effects of a PfFCP dominant negative mutant and found that it altered food vacuole morphology and trafficking. A previous report localized the …
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Characterization of a glycerophosphodiester phosphodiesterase in the human malaria parasite Plasmodium falciparum
… within the parasite cytosol, parasitophorous vacuole and food vacuole. To gain insight into the role of PfGDPD, sub-cellular localization was modulated and resulted in a shift in protein distribution, which elicited no growth phenotype. Kinetic analyses suggest PfGDPD activity is Mg₂⁺ …
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Chloroquine accumulation in Plasmodium falciparum isolated digestive vacuoles
… intact Plasmodium falciparum isolated digestive vacuoles, the site of chloroquine accumulation and action. Marker enzymes and gel electrophoresis were used to demonstrate purity, and electron microscopy to verify integrity of isolated vacuoles. Using this method, vacuoles were isolated in a yield …
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Characterisation of Mefloquine accumulation in Plasmodium falciparum
… dependent on the pH gradient between the acidic food vacuole and the external medium. It has also been determined that mefloquine-resistant Plasmodium falciparum accumulate approximately half the amount of mefloquine than do mefloquine-sensitive parasites. It has been shown that the accumulation of …
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Influence of protozoan grazing on the marine geochemistry of particle reactive trace metals
… enzyme-rich microenvironment of the grazer food vacuole. This thesis examines the role of protozoan grazers in the marine geochemistry of strongly hydrolyzed, particle-reactive trace metals, in particular Th and Fe. A series of tracer experiments was carried out in model systems in order to …
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Gene expression profiling of polyamine-depleted Plasmodium falciparum
… within the parasite seems to result in increased food vacuole acidification, haemoglobin degradation, transport of proteins to the cytoplasm and protein synthesis and stabilisation. The majority of downregulated transcripts were found to be involved in cell-cell adhesion and erythrocyte invasion, …
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Purification and Characterization of Native and Recombinant Dipeptidyl Aminopeptidase 1 of Plasmodium falciparum
… dipeptides from globin oligopeptides in the food vacuole. The lack of success at DPAP1 gene disruption suggests that this exopeptidase is important for efficient growth during the erythrocytic asexual stage. DPAP1 is therefore an attractive target for the development of anti-malarial drugs …
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Investigation of azithromycin analogues and proteasome-like inhibitors as quick-killing antimalarials
… acts multi-factorially, with the parasite’s food vacuole and mitochondria being likely targets. Finally, in vitro activities of two subtypes of tri-peptide proteasome-like inhibitors, vinyl sulfone and aldehydes, were addressed against P. falciparum and the zoonotic malaria parasite P. …
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Evaluation of antihistamines for in vitro antimalarial activity against Plasmodium falciparum
… haemoglobin localization in the parasite’s food vacuole. Western blots have confirmed these results, in addition to indicating that chlorpheniramine decreases the haemoglobin content in the parasite. The results confirm that certain antihistamines do indeed cause a reduction in the growth of …
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Synthesis, characterisation and pharmacological evaluation of silicon-containing aminoquinoline organometallic complexes as antiplasmodial, antitumor and antimycobacterial agents
… 13-haematin through superior accumulation in the food vacuole of the parasite, and therefore better at killing the malaria parasite. All compounds exhibited weak to moderate antitumour activities. The studies also showed that the ruthenium metal complexes generally display better activity in …
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Synthesis and investigation of benzimidazole and carbazole ß-haematin inhibiting scaffolds with antimalarial activity
… in inhibition of haemoglobin uptake into the food vacuole and not via haemozoin inhibition.