Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"follicle cells"”.
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Exploring Polarized Membrane Protein Distribution in Drosophila Follicle Cells using Proximity Labelling
… of apical–basal polarity in epithelial cells depend on the faithful sorting and delivery of proteins to distinct plasma membrane domains. Using the Drosophila follicular epithelium as my model, this thesis applies peroxidase-mediated proximity labelling and mass spectrometry to …
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Gene amplification in Drosophila ovarian follicle cells as a developmental strategy and model for metazoan DNA replication
… encoding the eggshell (chorion) proteins in the follicle cells of each egg chamber are overreplicated 20 or 60 fold (for the X chromosome and third chromosome amplicons, respectively). Gene amplification is accomplished using the normal eukaryotic DNA replication machinery and a bidirectional DNA …
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Characterization of the Drosophila Egfl7/8 ortholog during oogenesis
… processes such as elimination of damaged cells or morphological shaping. Apoptosis can be induced by intrinsic signals generated within the cells or from extrinsic signals received from the surrounding environment. This work centered on the analysis of the mechanisms and signals that …
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Analysis of metazoan DNA replication initiation using Drosophila gene amplification as a model system
Gene amplification in Drosophila follicle cells is an excellent model to study origin specification and developmental regulation of DNA replication in vivo. We mapped all follicle cell amplicons using a comparative genomic hybridization strategy and identified two new amplicons. We determined the …
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A Genetic Screen for Tribbles Suppressors Identifies the E3 Ubiquitin Ligase Neuralized as a Novel Target
… generates a distinct visual phenotype; larger cells in the posterior of the wing and overall smaller tissue size. Co-misexpression of the known targets of Tribbles slbo (Slow Border Cells) and string suppresses the Tribbles wing phenotype. We used this observation as a tool to screen for …
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Investigating the underlying mechanisms of axis formation in Drosophila melanogaster
… these underlying mechanisms, studies of both the follicle epithelium and germline were undertaken. Posterior follicle cells are known to be responsible for the formation of both body axes through signalling, but very few factors have been identified which perturb this process. The transcriptomic …
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Investigation of the role of VML in the establishment of Drosophila embryonic dorsal-ventral polarity
… the expression of the gene pipe in the ventral cells of the follicular epithelium surrounding the developing oocyte. pipe encodes a sulfotransferase that transfers sulfate groups to several protein components of the vitelline membrane layer of the eggshell, including Vitelline Membrane-Like …
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Analysis of the role of Drosophila IMP in actin mRNA localization and cell migration
… IMP is required for border cell migration and follicle cell morphogenesis during oogenesis. Later on, imp null embryos do not complete embryogenesis and fail to undergo dorsal closure. One of the working models for IMP function is that it localizes actin mRNA to the leading edge of migrating …
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Transcriptional regulation of the piRNA pathway in animal ovarian germ cells
… The enforced expression of Ovo in somatic cells activates expression of germline piRNA pathway components, including the ping-pong factors Aubergine, Argonaute-3 and Vasa, leading to assembly of peri-nuclear cellular structures resembling nuage bodies of animal germ cells. In the second …
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ΣΥΓΚΡΙΤΙΚΗ ΜΕΛΕΤΗ ΤΗΣ ΧΟΡΙΟΓΕΝΕΣΗΣ ΣΕ ΕΝΤΟΜΑ ΟΙΚΟΝΟΜΙΚΗΣ ΣΗΜΑΣΙΑΣ
… VITELLITE MEMBRANE) AND THE OVERLAID EPITHELIUM (FOLLICLE CELLS) HAS BEEN EXTENSIVELY USED STRUCTURALLY AND MOLECULARLY AS A MODEL SYSTEM FOR STUDIES ON CELL DIFFERENTIATION. THE MOST THOROUGHLY STUDIED EGGSHELL BELONGS TO THE FRUIT FLY DROSOPHILLA MELANOGASTER (DIPTERA: DROSOPHILIDAE). THE ENZYME …
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Developmental regulation of DNA replication initiation in Drosophila
Developmental gene amplification in the ovarian follicle cells of Drosophila provides a powerful system for the study of metazoan DNA replication. Amplification produces 100kb gradients of amplified DNA through repeated rounds of origin firing and bidirectional movement of replication forks from …
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Investigating transcription factor dynamics and chromatin environment during Notch response
… chambers, where Notch is endogenously active in follicle cells during a limited period (Stages 5-8). In this context, single molecule FISH was used to probe transcription of Notch targets. Unlike in the salivary gland, Trr was dispensable for normal profile of expression in the follicle cells. …
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Drosophila Cyclin J And The Somatic Pirna Pathway Cooperate To Regulate Germline Stem Cells
… chambers containing an overabundance of germline cells, while double mutants of CycJ and piwi or armi had more severe defects. CycJ mutants also genetically interacted with mutants of the germline piRNA pathway member aub, resulting in unique oogenesis defects, though they were less severe than …
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Morphogenesis of follicular epithelium in Drosophila melanogaster: function of von Hippel-Lindau tumour suppressor gene
… of the differentiated phenotype in renal cells and regulation of epithelial-mesenchymal transition. However, the function of h-VHL gene during development has not been fully understood. It was previously shown that d-VHL down-regulates the motility of tubular epithelial cells (tracheal …
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Untersuchungen zur Wirkung von östrogenwirksamen Stoffen auf die Gonadenentwicklung bei Fröschen (Rana temporaria und Xenopus laevis) sowie ultrastrukturelle und immunhistochemische Untersuchungen an Froschgonaden (Rana temporaria)
… by an epi-thelium and filled with germ cells which enclosed an ovarian cavity. The oogonies were mainly found in the peripheral parts of the organ. The oocytes were characterised by a big and irregularly shaped cell nucleolus with peripherally located nucleoli. They were sur-rounded by a …
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The Roles of the Drosophila Protein Tribbles in Oogenesis and Insulin Signaling Pathway
… Trbl localizes to the nucleus of main body follicle cells (MBFC) up to stage 10 of oogenesis. In the case of BC, Trbl expresses in a complementary pattern to Slbo expression. The Trbl level decreases gradually as the BC cluster delaminates from the epithelium and starts migrating when Slbo …
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Deciphering the Functional Collaboration of Mid and bric-a-brac 2 as Potential Regulators of Cellular Proliferation Within Adult <i>Drosophila</i> Ovaries
… ovary including the germline stem cell (GSC), follicle stem cell (FSC), and escort stem cell (ESC) niche. Recently, Fregoso-Lomas et al. [2] reported that Gurken/Epidermal Growth Factor Receptor (EGFR) signaling is modulated within posterior ovarian follicle cells by Midline (Mid). The …
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Decoding Mitochondrial Heteroplasmy: Illuminating Regulatory Pathways via Rpn11, Mocs3, and Beyond
… By examining 3,000 genes in cultured Drosophila cells stably transmitting a detrimental mtDNA mutation and quantify heteroplasmy levels via qPCR, I found that knockdown of ~1% of them can alter the heteroplasmy level during mitotic divisions. Among these candidates, MOCS3, a …
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Delineation of the molecular mechanisms underlying DNA replication initiation and changes in gene copy number during Drosophila development
… We then utilized the Drosophila amplicons in follicle cells (DAFCs) as a model replication system to dissect the molecular mechanisms underlying the activation of individual replication origins. Repression of the DAFC-22B origin is not achieved through changes in subnuclear localization but …
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Drosophila E2F and DP genes : their role in the regulation of G1-S progression and the activity of DNA replication origins
Late in Drosophila embryogenesis the cells giving rise to most of the larval tissues switch from a mitotic cycle to the endo cycle. The endo cycle consists of only S phase and a gap phase, leading to polyteny. It is preceded by the first detectable G1 period in Drosophila embryogenesis and …
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