Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 17 of 17 for “"folding kinetics"”.
-
Millisecond and Submillisecond Folding Kinetics of Horse Apomyoglobin and Yeast Phosphoglycerate Kinase
… tryptophans mutants also showed vastly different folding times indicating considerable folding heterogeneity both interdomain and intradomain. Furthermore, by varying the final folding temperature and thereby adjusting the native bias, the kinetics were successfully tuned between type 1 …
-
Advancing fast relaxation imaging to determine protein stability and folding kinetics on poly(n-isopropyl acrylamide) films
… affects protein thermodynamic stability and kinetics. Fast Relaxation Imaging (FReI) was applied to quantify the folding stability and dynamics of proteins in contact with PNIPAM at varying grafting conditions categorized as brush, weakly overlapping, and mushroom regime. FReI detects protein …
-
Protein folding and diffusion: from in vitro to live cells
Protein folding landscapes and protein-protein interaction landscapes are subject to modulation by many factors inside living cells: crowding, electrostatics, hydrophobic interactions, and even hydrodynamic phenomena. The resulting spatio-temporal fluctuations in protein folding rates, protein …
-
Folding Studies on Mutants of Chymotrypsin Inhibitor 2
The thermodynamics and folding kinetics of mutants at the helix N-terminus and hydrophobic core of Chymotrypsin Inhibitor 2 (CI2) have been studied. All mutants adhere to a two-state model for protein folding , and are destabilised relative to wild-type. Mutation of N-cap residue S31 to Ala or Gly …
-
Fast Protein and RNA Folding on a Rough Energy Landscape
… approach with other experimental methods, the folding process of protein assembly, protein molecules, and RNA molecules was focused in this work. For the first time, time-resolved self-assembly of a light-harvesting protein complex was studied using the laser T-jump method. Experiment results …
-
Protein folding in crowded environments and living cells
… this divide by performing studies of protein folding in in vitro crowded environments and in living cells. We begin by investigating the thermodynamic and kinetic behavior of the proteins in protein/carbohydrate matrices, and find significant differences compared to dilute, buffer solutions. …
-
RNA secondary structure prediction using hybrid methods
… structure assembly. Drawing insights from RNA folding kinetics, the stage-1 (S1) model proposes local substructures as pixel-level square bounding boxes. The subsequent stage-2 (S2) models, including the scoring network (S2SC) and the encoder-decoder transformer (S2ED), leverage recurrent …
-
Frustration of protein folding from in vitro to in vivo
Protein folding, a ubiquitous and vital biological process, where protein random coil transforms into certain conformation in order to fulfill its function. Misfolded protein which fails to acquire proper shape, not only loses its function, but can also cause fatal diseases. In this dissertation, I …
-
Investigating asparagine-linked glycosylation substrate : specificity and effects on protein folding
… the effects of N-linked glycosylation on protein folding, a semi-synthetic strategy was developed to access a set of model proteins that were homogeneously glycosylated at several sites of interest. The folding kinetics of this set of glycoproteins were then characterized using stopped-flow …
-
Observing molecular interactions that determine stability, folding, and functional states of single Na+/H+ antiporters
… in the native lipid bilayer caused a step-wise unfolding of the protein and provided insights into its stability. Modified experiments allowed observing refolding of NhaA molecules and estimating folding kinetics for individual structural elements, as well as detecting eventual misfolded …
-
COMPUTATIONAL APPROACHES FOR PROTEIN FOLDING AND LIGAND BINDING: FROM THERMODYNAMICS TO KINETICS
… both governed by biomolecular thermodynamics and kinetics. In order to make meaningful and efficient predictions of these mechanisms, molecular simulations must be able to estimate the binding affinity and rates of association and dissociation of a protein-ligand complex, or the populations and …
-
The nanomechanics of polycystin-1: A kidney mechanosensor
… impact on both the mechanical stability and refolding rate of PKD domains. It not only lowers their mechanical stability, but also slows down their refolding rate. Moreover, several osmolytes were found to effectively counteract the effects of urea. Our data provide the evidence that naturally …
-
Investigating Protein Folding Pathways at Atomistic Resolution: from a Small Domain to a Knotted Protein
Although protein folding has been studied for decades many open issues still resist, and we yet lack a clear and general description of the mechanisms leading from the unfolded to the folded state. In particular, it is still under debate whether proteins fold through few well-defined pathways or …
-
Kinetic Characterization of the Coupled Folding and Binding Mechanism of Bacterial RNase P Protein: an Intrinsically Unstructured Protein
… distinguishable states present in a protein folding pathway provides not only the kinetics and energetics of protein folding but also insights into the functional roles of these states in biological systems. The protein component of bacterial RNase P holoenzyme from Bacillus subtilis (P …
-
Protein folding in living cells and under pressure
Protein folding, the process through which proteins gain their functional structure, can be approached from the perspective of many disciplines. Starting with biology, we can probe how protein structure relates to function and consider how the fold of a protein interacts with the biological …
-
Spontaneous Unfolding and Refolding of FNIII Domains Assayed by Thiol Exchange
… of the fibrils. The first model: the ‘domain unfolding’ model postulates that the unraveling of FNIII domains under tension explains fibril elasticity.</p><p>The second model relies on the conformational change of FN from compact to extended to explain fibril elasticity. FN contain 15 FNIII …
-
Computational investigations of protein dynamics and its implications for biological functions
… conformational changes in proteins, including folding, dimerization, and unfolding, and how these conformational changes help the proteins perform their biological functions. We first study protein folding, a process by which a protein acquires its functional structure and is related to many …