Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 263 for “"fluorophore"”.
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Mass Spectrometric Characterization and Fluorophore-Assisted Light Inactivation of Human Excitatory Amino Acid Transporter
Glia-expressing excitatory amino acid transporter 2 (EAAT2) mediates the bulk of glutamate re-uptake in the human central nervous system (CNS) and is associated with a variety of neurological disorders. Our understanding of the structure and mechanism of this integral membrane protein is limited. …
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Mass Spectrometric Characterization and Fluorophore-Assisted Light Inactivation of Human Excitatory Amino Acid Transporter
Glia-expressing excitatory amino acid transporter 2 (EAAT2) mediates the bulk of glutamate re-uptake in the human central nervous system (CNS) and is associated with a variety of neurological disorders. Our understanding of the structure and mechanism of this integral membrane protein is limited. …
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New tools derived from the solvatochromic 4-N,N-dimethylamino-1,8-naphthalimide fluorophore for the detection of biomolecular interactions
… 4-N,Ndimethylamino- 1,8-naphthalimide (4-DMN) fluorophore for the study of biomolecular interactions. This fluorophore is distinctive in that it yields very few compromises by combining many of the key elements required of a good fluorescent tool.
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Funktionalisierte OS(T)K-Stäbe und Sensorfluorophore zur optischen Sensorik
… (FRET) und zum anderen Sensorfluorophore, basierend auf einem DBD-Fluorophor und BAPTA, zur Messung der intrazellulären Calcium-Konzentration dargestellt. Für die Synthese von molekularen Sonden für abstandsabhängige Messungen wurden verschiedenste einfach- und …
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Fluorescent-Labeled Estrogen Receptors: Molecular Sensors of Receptor Structure and Function
By attaching the fluorophore directly onto the ligand-binding domain, we have created out of the ER a molecular sensor through which we can probe directly the conformational state of the receptor with high sensitivity. Thus, fluorophore-labeled ERs provide tools for studying receptor structure and …
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Cellular delivery and site-specific targeting of organic fluorophores for super-resolution imaging in living cells
… of such imaging modality is synthetic organic fluorophores with suitable brightness and photostability. However, organic fluorophores are very difficult to use in live cells because of their chemical compositions. Many excellent fluorophores, such as cyanine and Alexa Fluor dyes, are highly …
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Single molecule studies of rna-target interactions
… We have also investigated two instances, a fluorophore-binding aptamer and molecular beacon, where the folding of RNA can be used to detect an analyte. We have demonstrated that binding of the fluorophore, TAMRA, to an aptamer enhances the fluorescent properties of the fluorophore. …
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Biochemical Evaluation of SRH Analogs as Potential LuxS Inhibitors
… purified LuxS be conjugated with an appropriate fluorophore, likely via a cysteine residue. An appropriate LuxS variant i.e. LuxS Y71C to which a single fluorophore would attach was acquired after extensive troubleshooting. An appropriate fluorophore would be attached to this LuxS variant for …
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Fibre optic sensing techniques for the detection of lead (II) ions
… unstable conditions. The second reagent is a fluorophore whose fluorescence mechanism and affinity to lead ions are explained in detail. Based on its binding properties with lead, the sol-gel matrix is selected to encapsulate this reagent and a probe is thus constructed but in subsequent …
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Insights into the mechanism of action of quinoline antimalarials against Plasmodium falciparum revealed by novel fluorescent analogues and chemical proteomics
… (NBD) was identified as a suitable reporter fluorophore and was attached to the quinoline core by nucleophilic aromatic substitution. The length of the spacer chain between the quinoline and the fluorophore was varied by preparing NBD-labelled amino acids and their corresponding succinimidyl …
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Synthesis and characterization of BODIPY-α-tocopherol : a new ligand to explore the intracellular transfer of Vitamin E
… the more robust dipyrrometheneboron difluoride fluorophore (BDP-a-Tocs; Aex = 507 nm, Aem = 511 nm). C8-BDP-a-Toc 42c, having an eight-carbon chain between the chromanol and fluorophore, wa<; shown to bind specifically to a-tocopherol transfer protein with a dissociation constant of …
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Using photo-instability to quantify fluorophores and achieve super-resolution imaging
… techniques for localizing and quantifying fluorophores in biological imaging applications. Fluorophore photobleaching, blinking, binding, etc., is exploited to quantify and localize single fluorophores, even when their fluorescent images overlap those of nearby fluorophores. In the first …
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Capture and control of excitations
… solar concentrators, this is the use of tandem fluorophores. In this approach, the addition of a second fluorophore material increases the amount of sunlight that can be absorbed without interfering with the efficiency at which the first fluorophore collects solar photons. For signal …
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Design and synthesis of novel functionalized sensors for the biological imaging of zinc(II) and nitric oxide
… Sensing of Biological Zinc A ratiometric two-fluorophore sensing system has been developed by coupling Zinpyr- 1, a zinc-sensitive fluorophore, to the zinc-insensitive coumarin-343. The two fluorophores are bound by an ester linkage, which allows cell permeability; cytoplasmic esterases …
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Grenzen und Möglichkeiten des Förster Resonanz-Energietransfer : einzelmolekülfluoreszenzspektroskopische Untersuchung an starren Systemen
… können aus den FRET-Effizienzen die Abstände der Fluorophore mit hoher Sensitivität berechnet werden. So wird FRET vielfach zur Abstandsmessung in biologisch relevanten Molekülen eingesetzt. Viele Informationen über die Funktion von Biomolekülen können bereits aus relativen Abstandsmessungen …
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Development and evaluation of a fluorescence emission ratio-based fiber opticpH measurement system for use in monitoring changes in tumor pH during clinical hyperthermia
… and temperature of the samples as well as by fluorophore leakage, photobleaching, and fluctuations in the intensity of the excitation source. The ratio technique minimizes the above sources of measurement error, but the need for a sequential excitation results in instrumentation that may be …
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Dipyrrin complexes and their uses as self assembling materials
… Each compound prepared consisted of one BODIPY fluorophore and one, two or three mesogenic units based primarily on a cyanobiphenyl core. Initially, the mesogens were attached to the pyrrolic positions of the fluorophore, but it was found that mesogen attachment at the BODIPY 8-phenyl ring gave …
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Protein engineering design principles for the development of biosensors
… Reagentless biosensors, in which a reporting fluorophore is conjugated to a binding scaffold, can detect analytes of interest with high temporal and spatial resolution. However, because these biosensors require laborious empirical screening to develop, their adoption has been limited. Hence, …
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