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Showing 1 to 20 of 38 for “"fluorometry"”.
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Characterization of cardiac IKs channel gating using voltage clamp fluorometry
… develops a voltage sensor assay, voltage clamp fluorometry, to measure movements of the voltage sensor and explore changes to the voltage sensor induced by KCNE1 and disease-causing mutations. Chapter 1 validates this technique using mutagenesis to ensure the assay reports on voltage sensor …
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A Study of Anisotropic Molecular Rotations by Differential Polarized Phase Fluorometry
Made available in DSpace on 2014-12-14T04:27:46Z (GMT). No. of bitstreams: 1 7913655.pdf: 3356121 bytes, checksum: 81f1468e0929ce4da88ba9de7ea1c59d (MD5) Previous issue date: 1978
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Application of Time-Resolved Laser-Excitation Fluorometry to the Study Ofprimary Photosynthetic Processes
Made available in DSpace on 2014-12-10T19:07:14Z (GMT). No. of bitstreams: 1 7405586.pdf: 3973095 bytes, checksum: ea45a34060f9f8aacbe526e47340c267 (MD5) Previous issue date: 1973
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Digital frequency domain fluorometry and the study of Hoechst 33258 DYE-DNA interactions
… can be studied. The use of frequency domain fluorometry to measure the lifetime of the excited state has been used for many years. Previously, its use was limited by the constraints of analog electronics. These electronics restricted the type of detector used, and because of the relatively …
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Investigation of primary productivity along the Atlantic Meridional Transect (50°N to 33°S) through fast repetition rate fluorometry
… in situ data from fast repetition rate (FRR) fluorometry. Samples were collected along the Atlantic Meridional Transect cruise 11 (AMTll, September-October 2000), between 50°N and 33°S in the Atlantic Ocean. Because of the large-scale nature of the study, consistent knowledge about …
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A Comparative Study of Three Bacterial Source Tracking Methods and the Fate of Fecal Indicator Bacteria in Marine Waters and Sediments
… Bacteroides human-specific primer test, and fluorometry were compared against the FIBs to determine how reliable each method was in detecting the presence of human fecal contamination. Two phases (Summer 2009 and 2010) were evaluated based on the type of contamination event. A combined sewage …
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Evaluation, Development and Improvement of Genotypic, Phenotypic and Chemical Microbial Source Tracking Methods and Application to Fecal Pollution at Virginia's Public Beaches
… of antibiotic resistance analysis (ARA) and fluorometry (to detect optical brighteners in detergents) were used in the summers of 2004 and 2005 to determine the origins of fecal pollution at beaches with a past history of, or the potential for, high enterococci counts and posted advisories. …
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Pressure Effects on Liposomes, Biological Membranes and Membrane-Bound Proteins
… were examined by steady state polarization fluorometry under pressure in the range of 1 bar to 2 Kbar. Isothermal pressure-induced phase transitions were observed in dipalmitoylphosphaditylcholine and dimyristoylphosphatidylcholine vesicles incorporated with 1,6-diphenyl-1,3,5-hextriene …
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Microbial Source Tracking in a Mixed Use Watershed in Northern Virginia
… the use of optical brighteners in freshwater by fluorometry as an indicator for human-origin pollution. One site on each of six streams and two sites on the remaining two (ten total) were selected for E. coli and Enterococcus monitoring and microbial source tracking. Repeated sampling of the ten …
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Headwater Stream Biofilm Structure and Function at High Resolution Spatial-temporal Scales.
… patterns in Shannon-Wiener diversity and in situ fluorometry chlorophyll-a in response to variable discharge-nutrient regimes demonstrating the ability of benthic diatom communities to recover from event-driven disturbances to their underlying equilibrium with water quality. This highlights the …
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Rock inhabiting and deteriorating fungi from carbonate monuments of Persepolis
… experiments by two methods; plate count agar and fluorometry. During this work the fluorometric technique was improved and elaborated to assay the efficiency of chemicals on rock fungi.
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Use of Escherichia coli for Microbial Source Tracking in a Mixed Use Watershed in Northern Virginia
… high rate of false negatives (negative fluorometry readings not supported by ARA), fluorometric analysis maintained a low rate of false positives (positive fluorometry readings not supported by ARA) and continued to demonstrate its potential for source tracking. This project represented …
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Airway inflammation, diagnosis, perception of asthma, and sputum zinc levels in a community cohort.
… laboratory has developed a novel method, Zinquin fluorometry, allowing measurement of labile zinc in body fluids. The final two studies demonstrate that IS lends itself to labile zinc measurements. Zinquin fluorometry was optimised to measure free pools of zinc in sputum. It was then used to …
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Studying the Structural Dynamics and Aptamer-Ligand Interactions in the Cocaine-Binding Aptamer through Fluorescence Spectroscopy
… aptamers using biophysical techniques including fluorometry methods. The results discussed in this study demonstrate how intrinsic fluorescence of ligands was exploited for aptamer-ligand binding and thermal stability analyses. Furthermore, photoisomerization of stilbene coupled with …
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Locating Sources of Bacterial Contamination In the Toms Creek Watershed
… for presence of optical brighteners using fluorometry.</p> <p>We found significant spatial variation in the watershed and were able to identify one presumptive source, namely, septic systems.</p> <p>We found strong seasonal variation in bacterial concentrations where fecal coliform …
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Utilising Chlorophyll Fluorescence to Assess the Variability of Phytoplankton Biomass and Productivity in the North-West European Shelf Seas
… the different conditions. Fast repetition rate fluorometry (FRRf) measurements of photosystem II (PSII) characteristics during a spring bloom were shown to change with hydrography and community composition. Thus, providing further support that the physiological state of PSII can be used as an …
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Dry and back again: characterization of desiccation-associated differentiation of leaf tissues in Craterostigma pumilum Hochst
… observation and pulse-amplitude-modulation fluorometry were used to identify the critical water contents at which key physiological changes occur in leaves of C. pumilum and how this relates to desiccation-associated differentiation between leaf Tip and Base tissues. This was followed by …
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