Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"fluorogenic substrate"”.
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Synthesis and evaluation of aziridine and symmetry-based epoxide affinity labels for HIV-1 protease
… HIV-1 protease, based on an internally-quenched fluorogenic substrate. Of eleven compounds assayed for reversible inhibition, five demonstrated modest to potent activities (K$\rm\sb{i}$'s ranging from 70 $\mu$M to 10 nM).
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High-throughput screening for ADP-ribosyl hydrolase 3 inhibitors
… a high-throughput screen was conducted using a fluorogenic substrate to discover an inhibitor of ARH3. A total of 496 primary hits have been identified and will be further studied to validate their ability to inhibit ARH3.
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Investigation of the YefM-YoeBSa toxin-antitoxin systems as novel antibacterial targets
… the activity of YoeBSa1 led to the design of a fluorogenic substrate that can be used to screen for activators of this toxin in a high-throughput manner.
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The Role of The Plasminogen-Fibrin Interaction in The Regulation of Fibrinolysis
… labelled plasminogen. A synthetic, fluorogenic substrate for plasmin was employed in the kinetic studies. Both forms of plasminogen displayed a physiologically relevant affinity for fibrin and limited plasmic cleavage of fibrin created new binding sites for plasminogen. This …
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Suitability of RelBE and MazEF toxin-antitoxin systems as antibacterial targets
… antitoxin. Additionally, a kinetic assay using a fluorogenic substrate was designed for the ribonuclease toxin MazFSa and applied in a screen for peptide enhancers of MazFSa enzymatic activity. A highly sensitive radiometric gel-based assay allowed for the first detection of endogenous MazFSa …
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The synthesis, structural characterization and biological evaluation of potential chemotherapeutic agents
… in a bioassay using Z-Phe-Arg-NHMec as a fluorogenic substrate. Resveratrol is a naturally occurring phyloalexin found in several plants but mainly in the skins of grapes. It has been shown to be produced in response to bacterial and fungal infections. It has been shown to exhibit various …
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Isolation and characterization of starch, starch biosynthetic genes and protease inhibitors from Marama beab (Tylosema Esculentum)
… using the reverse zymogram technique and fluorogenic substrate N-alpha-benzoyl-l-arginine 7-amido-4-methylcoumarin hydrochloride and cDNA clone encoding a serine protease gene from marama was isolated using newly developed degenerate PCR primers. Native marama starch content was 87.38 mg …
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Purification and Characterization of Native and Recombinant Dipeptidyl Aminopeptidase 1 of Plasmodium falciparum
… inhibitors, it is necessary to characterize the substrate specificity of this enzyme along with its human homolog cathepsin C. Although native purification of DPAP1 is possible, the amount of purified enzyme obtained is insufficient for extensive biochemical characterization. To overcome this …
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Studies on soil and microbial lipases
… lipases. Lipase activity was dependent upon substrate surface area rather than substrate concentration per se. Long-chain insoluble fatty acids were inhibitory to lipase activity. A minimum concentration of fatty acid was required before inhibition of lipase activity occurred. This minimum …
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Studies on oligopeptidase B of Leishmania major
… it was decided to use short peptidyl fluorogenic substrates, which only fluoresce once the bond linking the peptide to the fluorescent moiety is cleaved. These allow detection of peptidase activity by quantifying the release of the fluorescent moiety. Detection of peptidase activity in …
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Structural and biophysical analysis of the proteasomal deubiquitinase, UCH37
… Rpn11, to remove polyubiquitin chains from substrate proteins as they are unfolded and translocated into the proteolytic core of the proteasome, where proteins are cleaved into small peptides and then released for recycling by the cell. UCH37 associates with the proteasome via binding of its …
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Investigating domain-selective angiotensin converting enzyme inhibition and oxidative inactivation
… using a continuous assay with quenched fluorogenic substrate. The N-domain displayed nanomolar (Ki = 11.21±0.74nM) and the C-domain micromolar (Ki = 11 278±410nM) inhibition, thus 1000-fold selectivity. Residues predicted to contribute to selectivity based on the N-domain-33RE co-crystal …
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Mechanisms of polyphosphate degradation
… with alcohols, we created chromogenic and fluorogenic polyphosphatase substrates that allow one to measure real-time activity of either endo- or exopolyphosphatases, depending upon assay configuration. We confirmed the products' identities through 1D and 2D 31P, 1H, and 13C NMR analyses. In …