Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"fluorescent sensors"”.
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Fluorescent sensors for metal ions
The considerable current interest in the use of fluorescent molecules in molecular recognition is not new, but is one that has increased in importance in recent years due to their applications in many fields including trace analysis in chemistry and biochemistry. Using the "dual fluorescence" …
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Novel Fluorescent Sensors for Dynamic Intracellular Signalling
… such experiments. This thesis presents a novel fluorescent reporter for PKC activity in living cells. The kinase C probe (KCP-1) consists of a PKC specific substrate sequence that is flanked by two fluorescent proteins, which are capable of fluorescence resonance energy transfer (FRET). The …
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Long-wavelength, reaction-based, fluorescent sensors for HNO and mobile zinc
… Having access to red and near-infrared (NIR) sensors is particularly useful for investigating the interplay of multiple analytes using fluorescence microscopy in conjunction with other probes that emit at shorter wavelengths. Chapter 2. Synthesis and Characterization of a Fluorescent Sensor …
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Investigation of zinc metalloneurochemistry with fluorescent sensors based on fluorescein platforms
… and highlight recent advances in the use of fluorescent sensors to study the neurotransmitters zinc and nitric oxide. Chapter 2. Fluorescent Sensors for Zn2' Based on a Fluorescein Platform: Synthesis, Properties and Intracellular Distribution Two new fluorescent sensors for Zn2' that utilize …
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Development of chemical tools for studying biological aldehydes: Enzyme substrates and fluorescent sensors
… First, we highlight our development of a fluorescent substrate for an aldehyde processing enzyme, aldehyde dehydrogenase 1A1 (ALDH1A1). ALDH1A1 belongs to a family of enzymes that process endogenous and exogenous aldehydes to carboxylic acids, detoxifying reactive aldehyde species and …
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Single-walled carbon nanotubes as near infrared fluorescent sensors : characterization, biological and analytical applications
… them to serve as nIR single molecule optical sensors capable of long term and stable operations in vitro and in vivo. In this thesis, we show that the 1D quantum confinement of photogenerated excitons in SWNT can amplify the detection of molecular adsorption to where single-molecule …
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Development of selective high-affinity fluorescent sensors for imaging neurotransmitters and application in cells and tissue
Herein, we develop a series of selective fluorescent sensors based on a quinolone core designed to image neurotransmitters catecholamines, serotonin and glutamate. Included are the main design strategy, synthesis, spectroscopic data (UV/Vis and fluorescence) and their applications in cells and …
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Intracellular sensor spatial multiplexing via RNA scaffolds
… the limitations of spectrally multiplexing sensors, fluorescent sensors are clustered by type and spatially separated in the cytoplasm to avoid cross-talk. Each sensor is fused to an orthogonal viral capsid protein that binds to a long, repetitive strand of its corresponding RNA sequence. …
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Fast image and data processing methods for novel neuroscience technologies
… neurons by shape or location alone. We propose a fluorescent in situ hybridization (FISH) based pipeline for reading out gene expression from neurons. Using optimization methods, we select a compact set of genes that provide enough information to distinguish every neighboring pair of neurons in …
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Design and synthesis of novel functionalized sensors for the biological imaging of zinc(II) and nitric oxide
CHAPTER 1: Fluorescent Sensors for the Biological sensing of Zinc(II) A variety of fluorescent techniques have been developed for the in vivo sensing of Zn2+. This chapter presents a brief overview of techniques used to image chelatable zinc(II) in neuronal cells. Ratiometric sensors have been …
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Development of Endoplasmic Reticulum Targeted Probes and Red Fluorescent Probes for Detecting Zinc
… is important in various biological functions. Fluorescent sensors based on circularly permuted fluorescent proteins (cpFPs) have been previously made to detect labile, or unbound, Zn<sup>2+</sup> within the cytoplasm of cells. These sensors have proven invaluable for studying Zn<sup>2+</sup>, …
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The detection of nitric oxide and its reactivity with transition metal thiolate complexes
… To this end, we seek to create tools for the fluorescent imaging of NO in vivo. We have adapted an existing small molecule-based sensor for more biologically relevant applications by including it within a polymeric film. We have also developed turn-on fluorescent sensors for NO based on …
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Development of Boronic Acid-Based Spectroscopic Sensors for Saccharides
… as a recognition moiety for the development of sensors for saccharides, due to its unique strong and reversible interaction with diols. The fluorescence and/or UV spectroscopic method are the easiest and most common methods to signal the binding event of these receptors (boronic acid compounds) …
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An improved understanding of fluorescent Zn(II) sensors and their uses in biological settings
Chapter 1. An Introduction to Fluorescent Zn(II) Sensors and Their Applications in Biological Systems This chapter opens with an overview of the numerous roles of zinc in biology, with an emphasis on labile Zn(II), that is, loosely bound or kinetically available stores of the ion. The synthesis, …
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Advancing fluorescence lifetime microscopy for quantitative bioimaging
… levels in the mitochondria were monitored using fluorescent sensors based on Förster resonance energy transfer (FRET). A published approach of delivering exogenous mitochondria to cells was applied to rescue some of the defects seen in the Alzheimer’s disease cell model. The capturing of multiple …
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Fluorescent and Colorimetric Biosensors Based on DNAzymes and DNA Aptamers
… applications. However, the number of successful sensors made from these nucleic acids is limited. On the other hand, technology for sequence-selective DNA detection has advanced remarkably. Two primary examples are molecular beacon-based fluorescent detection and nanoparticle-based colorimetric …
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Fluorescent Materials for Chemical Sensing
Materials incorporating fluorescent π-electron conjugated molecular species for sensing and imaging are advantageous over other detection or identification methods such as colorimetric reporters. Fluorescent materials offer easy recognition of binding events due to the highly sensitive nature of …
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I The synthesis and coordination chemistry of novel 1,1,1-tris(aminomethyl)ethane (TAME) derived ligands and their use as size selective fluorescent zinc(II) sensors II The synthesis of strongly and weakly binding ligands with nitrogen, oxygen, and sulfur-donor groups to be used as receptors in fluorescent ratiometric indicators for transition metal ions
… in the preparation of novel Zn(II)-selective fluorescent sensors. The molecules TAMEisoquin and 6,7-DMTI possess the fluorophore-spacer-receptor design motif that is evident in many photoinduced electron transfer (PET) based metal-ion sensors. These molecules display exquisite spectroscopic …
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Studies on the Synthesis, Function, and Properties of Troponoids
… leading to the discovery of a new class of fluorescent small molecule sensors. We also identified crucial deficiencies in our understanding of tropolone structural properties, necessary to the efficient development of troponoid based therapeutics.</p> <p>Chapter 2 describes our efforts in …
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Time to quit? Non-genetic heterogeneity in cell fate propensity after DNA damage
… tracing of cells expressing genetically encoded fluorescent sensors, particularly the Fluorescent Ubiquitination-based Cell Cycle Indicator (FUCCI) probes (Sakaue-Sawano et al., 2008), with semi-automated image analysis to characterise the response of single cells and their descendants to DNA …
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