Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 49 for “"fluorescent reporter"”.
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Simultaneous, Large Multi-Gene Delivery for Implementation of Fluorescent Reporter Spatial Multiplexing to Image Signaling Pathways
… Today, many intracellular, genetically encoded reporters provide a readout in living cells using fluorescent proteins. Spectral multiplexing has been attempted by engineering reporters to each have a unique spectrum that does not overlap with other reporters to allow for simultaneous recording …
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Investigation of natural genetic modifiers of meiotic crossover frequency in Arabidopsis thaliana
… a defined region of the genome flanked by a fluorescent-reporter system. F1 plants were then self-fertilised to produce segregating mosaic F2 populations for mapping. Recombination frequency for specific genomic intervals was determined for each individual in the population through …
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Genetics and Evolution of Drosophila Humoral Immunity against Parasitoid Infections
… larval fat body after parasitism. Using a fluorescent reporter driven by the lectin-24A upstream region, I found that a deletion largely abolished lectin-24A expression in some natural populations susceptible to parasitoid infections. Infection by parasitoids induces an immune response in …
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Detection of Polypeptide Interactions Via Periodate Triggered Dopa Crosslinking
… chimeric molecule consisting of the biarsencial fluorescent reporter FLAsH conjugated to 3,4-Dihydroxyphenylalanine as a route to deliver 3,4-dihydroxyphenylalanine site specifically to a protein of interest and probe for protein-protein interactions partners in cell lysates.
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Correlating antisense RNA performance with thermodynamic calculations
… mRNA hybridization were computed based on the fluorescent protein reporter genes. Three different fluorescent proteins (i) green fluorescent protein (GFP), (ii) cyan fluorescent protein (CFP) and (iii) yellow fluorescent protein (YFP) were used as reporters. Each fluorescent protein was cloned …
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The localization, function and applications of the stress response protein Hsp12p in the yeast Saccharomyces cerevisiae
… of Hsp12p was determined by fusion to the green fluorescent reporter protein, Gfp2p and a combination of epifluorescent microscopy and confocal imagery. Chemical extraction revealed that Hsp12p was present in the cell wall while fluorescent imagery was not conclusive. This fluorescent Hsp12p …
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Netrin Guides Spinal Commissural Axons to the Midline at Long Range Through the Receptors Dcc and Neogenin
… interneuron populations in combination with fluorescent reporter lines, we show that midline guidance of all commissural interneuron populations wholly depends on Netrin-1 signaling through the Dcc and Neo1 receptors. However, the genetic deletion of Dcc more severely perturbed midline …
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INVESTIGATING MECHANISMS REGULATING MAMMALIAN SPERMATOGONIAL DIFFERENTIATION AND MEIOTIC INITIATION
… in mice. In chapter 3, I identify a new fluorescent reporter mouse model used to isolate large, highly enriched, precise population of germ cell at specific stages of their development and present a new culture system to study male germ cells in vitro. In chapter 4, I test the requirement …
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Genetic noise in the cyanobacterial circadian oscillator
… single cells, the expression fluctuations of a fluorescent reporter driven by the cyanobacterial circadian oscillator. Repeated microscopic imaging of individual cells and their progeny revealed a robust circadian rhythm, and experiments with cells lacking the proposed central clock proteins …
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Surface Displayed SNAP as a New Reporter in Synthetic Biology
… by visualizing an extracellular synthetic green fluorescent reporter, SNAP-Cell® 505-Star, simultaneously with an intracellular red fluorescent protein, mCherry. Moreover, we have shown that our construct enables cells to selectively bond to BG-conjugated magnetic beads. As a result, we have …
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Defining the mechanism(s) of Hepatitis C virus (HCV) entry
… for high-throughput screening, most cell-based reporter assays rely on the secretion of serine alkaline phosphatase and thus do not allow the selection of HCV infected cells, or the screening of patient samples to identify cell culture infectious viral strains. We aimed to develop a cell-based …
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The chronic response to centrosome loss-induced cell cycle exit
… bona fide senescence. I have used a novel fluorescent reporter of p53 transcriptional activity in RPE1 cells and combined it with live cell lineage analysis to reveal that p53 activation upon centrinone treatment is insufficient to commit cells to cease proliferation immediately. Instead, …
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Analysis of the Role of EIF5A in Mammalian Translation
… the 5' UTR of the transcript, we performed a fluorescent reporter-coupled genome-scale CRISPR/Cas9-mediated loss of function screen. Analysis of screening data identified eukaryotic initiation factor 5A (EIF5A) as novel regulator of MYC translation. eIF5A is a highly conserved translation …
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Quantitative Characterisation of Morphological and Phenotypic Changes During Microbial Cell Differentiation and Multicellular Behaviour
… These methods include monitoring expression of a fluorescent reporter for an operon whose gene products produce the polysaccharide component of the extracellular matrix, and doing so with strains deficient in the major matrix component genes results in establishing that regulation of this gene …
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Characterization of Negeviruses and insect virome interactions during co-infection in cell culture
… infectious clones of Negeviruses that have fluorescent reporter genes inserted into their genome. Analyzing the growth trends of different Negeviruses in these cell lines allow us to determine which Negevirus isolates can establish infection in the presence of existing or co-infections with …
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Potential-Dependent-Adsorption and Reorientation Responses Associated With a Phospholipid Monolayer at the Water /Dichloroethane Interface
… Mixed films of a phospholipid, DLPC, and the fluorescent reporter lipid di-N-butylaminonaphthylethenyl-pyridiniumpropylsulfonate, I, were assembled at this interface from the bulk organic and aqueous phases respectively. The mechanical (interfacial tension) properties of the interface were …
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Cell-cell communication in three dimensional microenvironments
… developed utilizing LPS and TNFα signaling with fluorescent reporter systems to monitor communication in real time. We demonstrated the utility of microfluidic devices to support the cell-cell communication model system by co culturing three cell types within Matrigel scaffolds and monitoring …
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Investigating dysregulation in protein quality control mechanisms in senescent cells
… as senescence onset, through proteomic and immunofluorescent techniques. We have characterised the proteome, poly-ubiquitylome, and insoluble proteome across different cell-states (proliferating, quiescent, and senescent) between two cell lines, primary human fetal fibroblast IMR-90s, and …
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CRAMP1 drives linker histone expression to enable Polycomb repression
… lines. Through a CRISPR/Cas9 genetic screen in a fluorescent reporter cell line responsive to PRC2 perturbation, I identified an essential requirement for the poorly characterised gene CRAMP1 in PRC2-mediated repression. CRAMP1 localises to the promoters of all expressed H1 genes where it acts as …
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