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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 73 for “"fluorescent proteins"”.
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Novel Design Strategies For Engineering Biliverdin-Binding Fluorescent Proteins
Fluorescent proteins (FPs) have revolutionized our ability to image biological systems. However, poor penetration and high autofluorescence of visible light in mammalian tissue has motivated the design of FPs that can take advantage of the minimal autofluorescence, scattering, and absorption in the …
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New directions in biological imaging: Engineering, characterization, and discovery of LOV-based fluorescent proteins
Made available in DSpace on 2014-05-30T16:49:00Z (GMT). No. of bitstreams: 3 Arnab_Mukherjee.pdf: 5391580 bytes, checksum: 4e1d0234958d4200ae65ae4e332e06b2 (MD5) Mukherjee_Arnab.docx: 21043138 bytes, checksum: 4cfe88d10b6cbe13ca845b27ee2e14c2 (MD5) license.txt: 4065 bytes, checksum: …
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Development of a Generalizable Assay for Probing the Effects of Mechanical Force on the Function of Fluorescent Proteins within Molecular Tension Sensors
… to measure forces experienced by specific proteins inside cells. However, an incomplete understanding of the effects of intracellular forces on fluorescent protein (FP) function within the context of MTs limits sensor application and interpretation. To advance our understanding of the …
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Functional Aspects of Protein Kinase C FRET Probe Performance
… PKC substrate pleckstrin, sandwiched between two fluorescent proteins, EYFP and GFP2. KCP-2 is a shortened version of KCP-1, missing the last 18 amino acids of the pleckstrin insert (the acidic loop). The EYFP/GFP2 emission ratio of KCP-1 increases upon phosphorylation, while it decreases in …
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Trimethoprim-Based Chemical Tags for High Resolution Live Cell Imaging
… have been developed to complement the use of fluorescent proteins in live cell imaging. Chemical tags retain the specificity of protein labeling achieved with fluorescent proteins through genetic encoding, but provide smaller, more robust tags and modular use of organic fluorophores with high …
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Experience-dependent dendrite remodeling of GABAergic interneurons in the adult visual cortex
… densities can be visualized by tagging fluorescent proteins to PSD95 and Gephyrin, respectively. Finally, we show that the fluorescent proteins, Teal and Venus, can be simultaneously excited and spectrally resolved through linear unmixing so that individual structural and synaptic …
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Expansion microscopy of C. elegans : nanoscale imaging of biomolecules throughout an entire organism
… elegans. ExCel enables simultaneous readout of fluorescent proteins, RNAs, DNA locations, and anatomical structures at resolutions of ~65-75 nm (3.3-3.8x linear expansion). We also developed epitope-preserving ExCel, which enables imaging of endogenous proteins stained by antibodies, and …
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A computational approach to spectroscopy of molecular systems : modeling, prediction, and design
… in influencing the absorption profiles of fluorescent proteins, utilizing machine learning on spectral clusters built from an ensemble of ground state dynamics trajectories. Using the example of green fluorescent protein, I show that this new feature selection protocol can highlight …
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Green fluorescent protein as a mechanical sensor
… structure dynamics. To this end, the green fluorescent protein (GFP) is considered as a mechanical sensor candidate with many desirable characteristics. Implicit solvent molecular dynamics CHARMM simulations demonstrated details inaccessible by AFM and OT methods, such as the linkage …
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Novel Bio-Imaging Techniques Based on Molecular Switching
… state and the first singlet excited state (the fluorescent state). Astonishingly, it greatly diversified the applicability of fluorescence microscopy in many different ways by incorporating additional molecular states and switching fluorescent molecules through these three or even more molecular …
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Exploring the Mitigation of TDP-43 Toxicity by Sis1 in Yeast
… cell. Sis1 is important in the degradation of proteins, and without it, the cell would become non-viable. In order to examine the relationship between the two proteins, they were tagged with fluorescent proteins and examined for colocalization under fluorescent microscopy. However, while TDP-43 …
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EVOLUTION AND CHARACTERIZATION OF A NEW REVERSIBLY PHOTOSWITCHABLE CHROMOPROTEIN
… will enhance future engineering of new and novel fluorescent proteins.
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Characterization of the DMCI and Rad51 homologues and the process of meiosis in trichomonas vaginalis
… capable of cytoplasmic fusion through the use of fluorescent proteins. Specifically, this study focuses on a line of Green Fluorescent Protein-expressing cells. </p>
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The Role of the SCD1 Protein in Targeting PIN2 Protein in Arabidopsis Thaliana
… the asymmetrical localization of auxin transport proteins in the plasma membrane. The <italic>SCD1<italic> (stomata cytokinesis defective) gene encodes a protein containing a DENN domain, which is predicted to function as a RAB-GEF, which may be important in membrane protein targeting. The …
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Engineered metalloproteins as contrast sensors for molecular fMRI
… modalities, genetically encoded sensors based on fluorescent proteins have provided an engineerable platform on which to optimize desirable device characteristics by exploiting the tools of molecular biology and protein biochemistry. Analogously, we seek to build genetically encodable sensors …
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Deterministic Barcoding of Neuron Identities through Multicolor Fluorescent Markers in C. elegans
… cell identification by generating a multicolored fluorescent map of different neurons in C. elegans. We take advantage of neuron type-specific promoters to construct transgenes that label each neuron type with unique red, green, and blue fluorescent colors. We also tag subcellular markers of the …
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Photoacoustic Tomography of Diabetes and Reporter Genes
… of contrast agents: melanin from tyrosinase and fluorescent proteins.</p>
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Applications of Sortase A from Staphylococcus aureus
… probes to biological molecules, such as proteins and DNA. This method does not require any toxic reagents or harsh conditions to achieve high level of labelling, and the product could be easily isolated from the reaction mixture. This new labelling techniques could potentially improve …
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Quantitative analysis of translational coupling in Escherichia coli
… of the upstream gene. This operon encodes two fluorescent proteins, where the upstream gene is translated by orthogonal ribosomes and the downstream gene by native ribosomes. This design allows us to precisely tune the translation of the upstream gene and then record its affect on the …
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