Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 76 for “"fluorescent probe"”.
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HIGHLY SPECIFIC FLUORESCENT PROBE FOR MOUSE PLURIPOTENT STEM CELLS
A highly specific fluorescent probe was discovered for mESC via high throughput screening of compounds. The hit compound was named CDy9. High throughput screen found 23 hit compounds that are specific to mESC. The cell panel test identified CDy9 as the most specific compound. FACS sorting of CDy9 …
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FLUORESCENT PROBE INVESTIGATIONS OF MICROENVIRONMENTS OF ANALYTICAL INTEREST (REVERSED-PHASE, POLYETHYLENIMINE, POLARIZATION)
<p>Fluorescent probes were applied to investigate three systems. Reverse phase chromatographic surfaces were studied using ion pairs. Variables were cation reagent structure and concentration, bonded phase (methyl, octyl, octadecyl, and phenyl), and solvent (water or methanol). Emission wavelength …
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Elucidation of chemically-induced transdermal transport processes
… B hexyl ester (RBHE), a model hydrophobic fluorescent probe, and of sulforhodamine B (SRB), a model hydrophilic fluorescent probe, based on a sampling of 4 to 6 different skin sites per skin sample. The fluorescent probe intensity profiles, that capture the fluorescent probe concentrations …
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Surface plasmon enhanced fluorescence for biological imaging : from visible to short-wave infrared
… and positron emission tomography. Imaging probes of high emissivity and photostability are the key to achieving fluorescence imaging with high signal-to-background ratio (SBR). One promising approach to developing highly bright and stable imaging probes is through surface plasmon enhanced …
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Synthetic Materials with Redox-Triggers for Imaging and Drug Delivery
… trigger was utilized to develop a novel NIR fluorescent probe, p-nitrobenzyl 3,7-bis(dimethylamino)-10h-phenothiazine-10-carboxylate for the selective imaging and therapy. Conjugating methylene blue with a p-nitrobenzyl moiety enables it to quench electron transfer within the molecules. …
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Preparation of 7-(p-Substituted-Benzylamino)-4-Nitrobenzoxadiazole Derivatives and the Study of their Fluorescent Properties in Solution
… and study their fluorescent behavior in aqueous-nonaqueous solvent systems. The parent compound has been used by others as a fluorescent probe to determine the conformation changes of bovine trypsinogen and bacterial libosomes. Fluorescent probes have been used to: …
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Structural and functional studies of heme binding proteins toward the understanding of malaria
… vacuole of Plasmodium. The second is mRuby2, a fluorescent probe often utilized in biological work to track the localization of proteins. Herein, we report on the novel heme-binding properties of both proteins. We have identified a novel function of mRuby2, a commonly used fluorescent probe. …
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THE STUDY OF PYRENEDIONE AS A PHOTOREDOX CATALYST AND A FLUOROMETRIC PROBE FOR VISUAL DETECTION AND QUANTIFICATION OF TRYPTOPHAN
… has been developed using 1,8-PYD as a turn-on fluorescent probe.
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Detection of Biologically Relevant Anions by Fluorescence and NIR Molecular Probes
… of biologically relevant anions using molecular probes. The first part has demonstrated that hydroethidine is oxidized to ethidium under the pro-oxidative effect of flavonols under physiological conditions and is a sensitive fluorescent probe for rapid quantification of the superoxide …
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Characterization of Homogeneous and Heterogeneous Anti-Nucleic Acid Autoantibodies in Systemic Lupus Erythematosus
… derivative was used relative to a mononucleotide fluorescent probe.
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The Spectroscopic Characterization Of Newly Developed Emissive Materials And The Effects Of Environment On Their Photophysical Properties
… pincer complex for blue emissive materials and a fluorescent probe for medical applications. The effects of noncovalent interactions are also investigated on these systems and a benchmark biological molecule trimethylamine N-oxide (TMAO).
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Regulation of Rubisco in Arabidopsis Thaliana Rca(-) Complemented With Arabidopsis Rubisco Activase Engineered at an ATP Binding Site
… of K$\rm\sb{d}$ ADP and K$\rm\sb{d}$ ATP with a fluorescent probe suggested that engineered Rubisco activase proteins have a reduced affinity for ATP and the inhibitor ADP. Transgenic plants expressing Q111D, Q111E, Q111S or 42kD wild type, showed a greater ability to activate Rubisco and an …
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Development Towards an Ethylene Sensing Artificial Metalloenzyme
… information about ethylene. A ruthenium based fluorescent probe for the selective detection of ethylene has recently been developed, and it was envisioned that this probe could be made biocompatible by encasing it within a protein scaffold making it an artificial metalloenzyme. To this end …
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An Investigation of the Phospholamban-Serca Regulatory Interaction with Fluorescence Resonance Energy Transfer
… move independently of its cytosolic domain. Our fluorescent probe is attached to the cytosolic domain of PLB, which would explain our inability to detect a change in the position of the transmembrane domain. My current work attempts to address this hypothesis by measuring the FRET between SERCA …
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STRUCTURE AND FUNCTION OF A NEURONAL GLUTAMATE TRANSPORTER
… during glutamate binding by incorporating a fluorescent probe into a site near the postulated external gate (HP2) of a mutant transporter that can bind but not transport L-Glu. Fluorescence changes were observed upon ligand binding that strongly depended on the number of subunits labeled; …
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STRUCTURE AND FUNCTION OF A NEURONAL GLUTAMATE TRANSPORTER
… during glutamate binding by incorporating a fluorescent probe into a site near the postulated external gate (HP2) of a mutant transporter that can bind but not transport L-Glu. Fluorescence changes were observed upon ligand binding that strongly depended on the number of subunits labeled; …
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Chemical probes for studying cyclooxygenase-2 and nitric oxide in living systems
… of binding-based and activity-based sensing probes to contextualize the following chapters. Chapter 2 discusses the design and validation of a fluorescent probe for detecting cyclooxygenase-2 activity with live cells, as well as the discovery of oxygen-dependent regulation that is not …
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New tools derived from the solvatochromic 4-N,N-dimethylamino-1,8-naphthalimide fluorophore for the detection of biomolecular interactions
… in real-time. However, the criteria for a good fluorescent probe are often numerous and demanding, particularly when considering applications in complex chemical environments like that of a living cell. Tradeoffs often exist between variables such as good extinction coefficients, suitable …
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