Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 103 for “"fluorescent microscopy"”.
-
A Comparison of Fluorescent Microscopy Methods for the Detection of Chlamydia trachomatis
… stocks and used to optimise and compare microscopy-based quantification methods. First, representative C. trachomatis urogenital serovars (E, H) and lymphogranuloma venereum (LGV) serovars (L1 and L2) were propagated in McCoy cells, using an established centrifugation protocol. These …
-
Quantitative, Competitive PCR and Fluorescent Microscopy Methods for the Study of Batrachochytrium dendrobatidis
… DNA sequence into a quantitative technique. Fluorescent microscopy is a vital tool used to study cellular and molecular biology. DNA and cell wall fluorescent staining of viable Bd observed with laser scanning confocal microscopy is described and should provide the foundation for future …
-
Quantitative Studies of Intracellular Trafficking of Two Classes of Resident Golgi Apparatus Proteins
… method for analysis of data collected using fluorescent microscopy. The second part of this dissertation presents results pertaining to the quantification of a unique Golgi resident protein that cycles in the late endosome bypass pathway. Using the published method of analysis and techniques …
-
The Impact of Acetylcholine Receptors on Osteocytes and Bone Mechanoadaptation
… We also used intravital imaging via two-photon fluorescent microscopy to visualize the Ca2+ dynamics in osteocytes during active mechanical loading of the bone in living mice. Finally, we explore the ability of light-sheet fluorescent microscopy to create high resolution 3D reconstructions of …
-
Increased Apoptosis in the Host By Guanidine Hydrochloride Treatment of Infectious Pancreatic Necrosis Virus
… detection were used: vital dye exclusion, fluorescent microscopy and DNA laddering in electrophoretic gels. The vital dye staining showed a significant decrease in viral activity post treatment with 4M GuHC1. Fluorescent microscopy results indicated examples of increased phosphatidylserine …
-
Ptychography for Nonlinear Optical Microscopy
… I will introduce a novel nonlinear optical microscopy method to address some of the shortcomings in the current nonlinear optical microscopy literature and offer a supplement to traditional fluorescent microscopy for label free optical biomedical imaging. In order to demonstrate this method …
-
Exploring the Mitigation of TDP-43 Toxicity by Sis1 in Yeast
… between the two proteins, they were tagged with fluorescent proteins and examined for colocalization under fluorescent microscopy. However, while TDP-43 does appear to titrate Sis1 out of the nucleus, there was no evidence that the two proteins colocalized.
-
Parallel and flexible fluorescent imaging using two-photon RESOLFT super-resolution microscopy with spatial light modulator control
… Saturable Optical Fluorescence Transitions) fluorescent microscopy is one technique which can achieve lateral super-resolution imaging. Two-photon microscopy naturally generate high resolution in the longitudinal direction with less background compared to single photon excitation. We combine …
-
Engineering morphogenesis of Marchantia polymorpha gemmae
… conditions. As such, they are well-suited for fluorescent microscopy and the collection of gene expression data. These features create an opportunity to study the processes of gemma morphogenesis at tissue, cellular and genetic levels through fluorescent microscopy in a single assay. In order …
-
The promise and perils of molecular combing
… labels them with an intercalating dye or fluorescent antibodies; and images the molecules with fluorescent microscopy. After combing, fluorescent in situ hybridization (FISH) can be used to inspect regions on the DNA molecule. DMC is especially technically challenging for undergraduate …
-
Transport of soft materials in crowded environment
Fluorescent microscopy was used to study the transport of soft material objects in crowded environment. Delaunay triangulation and wavelet transform were adapted to extract more information from images of macromolecules with irregular shapes and heterogeneous transportation dynamics. Systems …
-
Aquaporin-4 mediates blood brain barrier permeability during endotoxemia
… inflammation. Here, mice were i.v. injected with fluorescent tracers of low (1 kd) and intermediate (10 kd) molecular weights 24 hours after induction of endotoxemia with i.p. LPS. Fluorescent microscopy using tissue sections and cleared half-brains showed that endotoxemia increased tracer …
-
Cytokine-capture nanoparticles to quantify IL-2 concentrations within the immunological synapse during T cell activation
… the presence of any captured-cytokines using 3D fluorescent microscopy or flow cytometry. Microscopy of DO.11 cells labeled with cytokinecapture particles have shown successful detection of exogenous IL2. A comparison of existing patents with cytokine-capture technology revealed that although …
-
A potential route to hydrogel multifunctionalization utilizing encapsulation of acrylate-conjugated streptavidin
… the use of rhodamine-labeled streptavidin and fluorescent microscopy. Gels were incubated overnight in solution to determine diffusion. After 7 days, PEG showed no diffusion while dextran acrylate demonstrated 100% protein loss.
-
An evaluation of cytokine-capture nanoparticle technology : stepping from bench-space into potential markets
… the presence of any captured-cytokines using 3D fluorescent microscopy or flow cytometry. Microscopy of DO.ll cells labeled with cytokine-capture particles have shown successful detection of exogenous IL2. A comparison of existing patents with cytokine-capture technology revealed that although …
-
Adipose tissue transplantation improves metabolic, behavioural and histological parameters in leptin-deficient mice
… mice, which express nuclear enhanced green fluorescent protein (EGFP) at very high levels in all cells. Approximately 2 g of WAT were subcutaneously transplanted into the dorsal area of 7- to 12-week-old leptin-deficient mice (ob/ob -/-). A 22-week post-surgical follow-up study was conducted …
-
Adipose tissue transplantation improves metabolic, behavioural and histological parameters in leptin-deficient mice
… mice, which express nuclear enhanced green fluorescent protein (EGFP) at very high levels in all cells. Approximately 2 g of WAT were subcutaneously transplanted into the dorsal area of 7- to 12-week-old leptin-deficient mice (ob/ob -/-). A 22-week post-surgical follow-up study was conducted …
-
Development of microfluidic devices to maintain and interrogate tissue biopsies - biomedical applications
… histological examination, confocal imaging, epi-fluorescent microscopy were all performed to assess the viability of the tissue before and after interrogation by several apoptosis inducing and chemotherapeutic agents. Liver tissue samples were also disaggregated in situ on-chip into individual …
-
Signal Processing In Single Molecule Studies
… developed in our research using fluorescence microscopy, namely, spectral deconvolution in multi-color fluorescent microscopy systems; and fluorescent pattern recognition in the time domain using the correlation functions. In the third chapter, spectral deconvolution is demonstrated in …
-
The structure and function of a bovine salivary protein - BSP30b
… surface of olive oil droplets, as observed by fluorescent microscopy. Bacteria extracted directly from rumen samples indicate that the GFP_BSP30b fusion protein binds to a small number of selected bacterial strains in vivo. These results suggest that BSP30b could have a specific range of lipid …
Page 1 of 6