Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"fluorescent labeling"”.
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Probing the Structure of Apolipoprotein AI by Specific Fluorescent Labeling of Introduced Cysteine Residues
Based on this study and previous results, a model of lipid-free proapoAI was constructed. This model takes into account the elongated shape of proapoAI, indicates that the N- and C-terminal extremes of proapoAI are compact, the C-terminal region is within 40 A of the 5 Trp residues in the …
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Biotin and Fluorescent Labeling of Ribonucleic Acids Using T4 Rna Ligase (Rna Modification, Rna-Dna Hybridization)
… oligoribonucleotides are highly fluorescent and show no quenching due to attachment to the acceptor. Analysis of the tRNA and 5S RNA reactions by polyacrylamide gel electrophoresis demonstrated the incorporation of ('14)C-labeled biotin or a fluorescent dye into the RNA molecules. …
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Near-infrared emitting quantum dots for cellular and vascular fluorescent labeling in in vivo multiplexed imaging studies
… been limited by the paucity of suitable probes fluorescent in the near-infrared spectrum that is favored by tissue optics. This project attempts to address this problem by developing cellular and vascular fluorescent imaging probes comprised of semiconductor nanocrystals, or quantum dots (QDs), …
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Synthesis, characterization and fluorescence performance of LaF3:Ce3+ nanocrystals
Fluorescent labeling agents have greatly affected many biological applications in recent years. Among different types of labeling agents, Cerium-doped LaF3 nanocrystals (NCs) are considered to be a promising new class of luminescent materials. Usually, lanthanide-doped nanocrystals production …
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Enzyme Kinetics of Recombinant Human Arylsulfatase B (rhASB)
… lyase allows quantitation of CS/DS through fluorescent labeling and by capillary electrophoresis. In addition to a direct hydrolysis assay analyzed by High Pressure Liquid Chromatography (HPLC), this gives two potential methods of examining the kinetics of rhASB with both a monosaccharide …
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Engineering Fluorescently Labeled Human Fibroblast Growth Factor One Mutants and Characterizing Their Photophysics Properties Towards Designing FRET Assays
… we successfully designed and characterized fluorescently labeled hFGF1 tracers which span the visible light region of the electromagnetic spectrum for use in smFRET. Fluorescent labeling was achieved by utilizing the maleimide moiety of the dyes (Alexa Fluor, Cyanine and iFluor) to conjugate …
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Chronic stress-dependent activation of somatostatin neurons in the nucleus accumbens facilitates maladaptive eating behaviors
… neurons within the NAc to binge eating. Further fluorescent labeling quantification and cell-type-specific optogenetic manipulation will be needed to further delineate the role of SOM neurons in orchestrating the inhibitory components of stress-modulated reward circuitry.
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Osteoblast-like cell response to titanium coated with co-immobilized Elastin-like polymers containing integrin-binding and Statherin-derived biomimetic peptides
… spreading, and proliferation was assessed with fluorescent labeling of actin, vinculin, and/or nuclear DNA and viewed with a light microscope. ELISA was used to determine early- and late-stage osteoblastic differentiation by measuring alkaline phosphatase and osteocalcin, respectively. Our …
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Optimized Microbial Recombinant Production of HIV-1 Anti-Envelope Antibody Fragments with Applications to Single Particle Tracking of Virus Assembly
… recombinantly produce, purify and apply various fluorescent probes in vitro for the fluorescent labeling and study of the human immunodeficiency virus type 1 (HIV-1) envelope (Env) protein during HIV viral assembly. There remains insufficient knowledge about the molecular dynamics and …
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Study of altered metabolism in cancer by high content SRS microscopy
… analysis, like mass spectrometry, or require fluorescent labeling that can perturb metabolic processes. Recently developed high-content, high-resolution SRS microscopy enables single-cell metabolic analysis and I successfully applied this approach to identify and target metabolic pathways in …
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Heme Peroxidase Hpx-2 Protects Caenorhabditis Elegans From Pathogens
… no obvious morphological defects. In addition, fluorescent labeling of HPX-2 revealed its expression in the pharynx, an organ in which BLI-3 also resides. Interestingly, loss of HPX-2 increased intestinal colonization of <em>E. faecalis, </em>suggesting its role in the pharynx may limit …
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Fluorescence labeling and computational analysis of the strut of myosin's 50 kDa cleft.
… the dynamics of the strut region, a method of fluorescent labeling of the strut was discovered using the dye CY3. CY3 served as a hydrophobic tag for purification by hydrophobic interaction chromatography which enabled the separation of labeled and unlabeled species of S1 including a fraction …
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New Types of Morpho-Physiological Changes in Cells Exposed to Nanosecond Pulsed Electric Field
… data obtained from the nsPEF-exposed cells using fluorescent labeling and pharmacologic inhibition. The adapted labeling techniques take advantage of the nsPEF-induced cell permeabilization to induce staining of microvesicles and pseudopod-like blebs (PLB). The results show that microvesiculation …
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Lipid Targets of the Antimalarial Trioxanes in <i>Plasmodium falciparum</i>
… using microscopic visualization of fluorescently-labeled artemisinin derivatives. We further determined the ability of artemisinin to generate reactive oxygen species <em>in vitro</em> and <em>in vivo</em>.</p> <p>Microscopic imaging of a fluorescent …
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Components of the Membrane Attack Complex (Mac) of Human Complement: Functional Studies of Their Macpf Domains
… of C8αMACPF may insert into the membrane. Fluorescent labeling of residues in C8αMACPF was unable to produce reliable data to indicate membrane insertion.</p> <p>The final aim used chimeras containing TMH regions of C8β replacing TMH regions of the CDC Perfingolysin O (PFO) to determine if …
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PopZ and FtsZ coordinate polar growth termination and cell division in Agrobacterium tumefaciens
… We further optimized a suite of target-specific fluorescent labeling techniques which allow us to visualize morphological changes during essential cell processes (Howell, Daniel, and Brown, 2017). We use these techniques to dissect the contributions of PopZ and FtsZ to polar growth and cell …
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ANALYSIS BY CAPILLARY ELECTROPHORESIS WITH LASER-INDUCED FLUORESCENCE DETECTION: FROM SMALL MOLECULES TO WHOLE CELLS
… molecules, but because few analytes are natively fluorescent, it is necessary to bind them with fluorescent tags if LIF detection is to be employed. These tags increase sensitivity and provide additional separation from components of the sample that do not bind the tags. In this dissertation, four …
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Scalable Subcellular Resolution Mapping of the Human Brain
… To address the challenge of slow and nonuniform fluorescent labeling of tissues in 3D, we developed tissue-hydrogel transformation technologies known as ELAST and mELAST that transform previously weak and sometimes brittle brain tissue into tough, stretchable, and elastic tissue-gel hybrids. In …
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The spectral and photophysical characterization of water solubilizing metal phthalocyanines for highly sensitive detection in biological studies
… the work presented here to develop a series of fluorescent probes that would aid in the increased multiplexing capabilities allowing the ability to detect multiple targets in a single measurement. Typically, spectral discrimination has been used to identify each DNA fragment in sequencing or …
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