Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"fluorescent label"”.
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Single label fluorescent derivatization of peptides
A one step method for the attachment of a single fluorescent label to peptides through their N-terminus is described. The method employs fluorescein-5-isothiocyanate as the label of choice and a lower than normal derivatization buffer pH that discriminates against $\varepsilon$-amino derivatization …
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Microbead-Based Biosensing in Microfluidic Devices
… were pumped through the conduit and used as a fluorescent label to monitor binding to the bead. Three-dimensional finite element simulations were carried out to model the mass transfer and binding kinetics on the beads’ surfaces and within the porous beads. The theoretical predictions were …
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Investigation of Prestin Membrane Topology by Substituted Cysteine Accessibility Method.
… membrane impermeable thiol-reactive probe, and a fluorescent label, residues were identified as accessible (extracellular) or inaccessible (intracellular). The resulting fluorescence was quantified and analyzed. It was found that residue A170 is accessible, and that residues C124 and G366 are …
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Smart, biocompatible semi-conductor nanocrystal constructs designed for in-vitro imaging applications
… have unique optical properties that complement fluorescent proteins and organic fluorophores. Despite the widespread use as a fluorescent label in biological imaging studies, the types of biological questions answered by utilizing QDs have been limited due to crucial shortcomings. This thesis …
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On the Kinetics of Protein Misfolding and Aggregation
… T, to-date the most frequently employed fluorescent label molecule for bulk solution kinetic studies, can in the presence of potential amyloid inhibitor candidates be highly unreliable as a means to quantify the effect of the inhibitor on amyloid formation kinetics. In summary, the work …
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Synthesis of mannosylated peptides as components for synthetic vaccines
… thesis describes the synthesis of fluorescein labelled O-mannosylated peptides using either manual or microwave assisted solid phase glycopeptide synthesis (SPGS) on pre-loaded WANG resin. The mannosylated peptides thus prepared can be tested for their ability to bind mannose receptors on human …
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Design and Synthesis of Complex and Fluorescent Labeled Cellulose-Based Derivatives for Orally Administered Drug Delivery Systems
… derivatives using oxidation chemistry to produce fluorescent poly- and oligosaccharides (hydroxypropyl cellulose, hydroxypropyl methylcellulose, and hydroxypropyl beta cyclodextrin). Schiff-base chemistry was then explored to append a commercially available fluorescent label, Nile Blue. Due to the …
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The Design of Biodegradable Polyester Nanocarriers for Image-guided Therapeutic Delivery
… to range from 100-200 nm in size. They were fluorescently labeled with a hydrophobic dye 6-13 bis(triisopropylsilylethynyl) pentacene (TIPS pentacene) at an optimal loading of 0.5 wt% with respect to the core. Surfaces were successfully coated with streptavidin to be readily functionalized …
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Pump-free magnetophoresis for improved point-of-care diagnostics
… at 105 colony forming units (CFU/mL) with a fluorescent label in a pooled human urine sample. Chapter 3, describes improvements to the device described in chapter two. The limit of detection was improved by three orders of magnitude and calculated at 4.67 x 102 CFU/mL in pooled human urine, …
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Alterations in the phase behaviour of human γD-crystallin due to mutagenesis and chemical modification of the protein surface
… of HGD with a (hydrophobic) small molecule fluorescent label were examined using both experiments and simulations. By measuring the LLPS temperature for modified proteins in mixtures with native protein, it was possible to determine how surface anisotropy and the chemical properties of the …