Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"fluorescent intensity"”.
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A Study of the Effects of Titanium Dioxide Nanoparticles on the Fluorescent Intensity of Fluorescent Compounds in the Presence of Known Quenchers.
… Quenching, which is one of the basic problems of fluorescent measurements, was studied in the presence of molecular oxygen and methyl iodide. The rutile phase of titanium dioxide nanoparticles was synthesized by the acid hydrolysis of titanium isobutoxide at low temperatures with nitric acid. The …
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Budding yeast cell cycle analysis and morphological characterization by automated image analysis
… this method can also provide information about fluorescently labelled proteins, unlike flow cytometry, which can only measure overall fluorescent intensity. The information about yeast cell cycle stage and protein amount and localization can then be connected in order to develop a model of yeast …
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DEVLOPEMNT OF FLUORESCENT IMAGING TOOLS FOR LIVE CELL IMAGING
… cellular processes and behaviors in real time. Fluorescent live cell imaging presents an appealing approach to learn about cell dynamics and function by providing specificity and high resolution. However, one of the biggest limitations of these imaging modalities is penetration depth. With …
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Producing Data to Support the Detection and Understanding of Early On-Set Corrosion in Accelerated Weathering
… The studies focused on incorporating two main fluorescent probes into a few model epoxy and/or urethane coatings to detect X and Y axis specific pH changes occurring upon and above the substrate during pre-macroscopic corrosion. The primary fluorophore used was fluorescein, a pH sensitive probe …
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Dysregulation of of phospholipid-specific phagocytosis by B1 B cells in diet-induced obese mice
… that B1 cells can phagocytize polystyrene fluorescent particles, bacteria (Staphylococcus aureus, Escherichia coli), and even apoptotic cells. Nevertheless, little is known about the biological significance of this seemingly redundant function of B1 B cells as compared to that of …
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In vitro selection of aptamers using quantum dot-assisted capillary electrophoresis SELEX
… DNA sequences. Laser-induced fluorescence-CE and fluorescently labeled DNA allowed for real-time identification of DNA-target binding by fluorescence resonance energy transfer between the DNA fluorophore and the Qdots, changing the latter's fluorescent intensity. After five rounds of selection, …
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Real time PCR measurement by fluorescence anisotropy
… real-time PCR measurement is based upon the fluorescent intensity of either intercalating dyes or oligonucleotide probes. Intercalating dye methods suffer from a lack of binding specificity, while probe methods are expensive and require increased assay optimization. In this thesis, a new …
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Characterizing Changes in the Brain During Hydrocephalic Development and Exploring Potential Treatment Strategies
… claudin-1 (Cl-1) and zona occludin 1 (ZO-1) fluorescent intensity increased in P15 hydrocephalic animals compared to wildtype (Tmem67+/+). These cells also contain aquaporins (AQP), aquaporin-1 (AQP1) and aquaporin-4 (AQP4), important in regulating CSF and interstitial fluid (ISF). Increased …
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Chemokine Ligand-3 (CCL3) as a Novel Mediator of Inflammatory Bowel Disease Activity
… there was no significant difference between the fluorescent intensity of CCL3, CCR1 and CCR5 across IBD gut mucosa biopsies. However, the co-occurrence and correlation of CCR1 with CCL3 was significantly higher than that of CCR5 with CCL3. This suggested that the CCL3 mediated effect may have …
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Nociception and the Experience of Pain Signaling Due to Nerve Damage
… on the post synaptic level by comparing the fluorescent intensity mean (FIM) values of fluorescent antibody labeled NMDA receptors for glutamate and PSD95 clustering proteins for NMDAr at integration and motor synapses of spinal cord tissues. The FIM values were then correlated to the …
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Fluorescence labeling and computational analysis of the strut of myosin's 50 kDa cleft.
… the dynamics of the strut region, a method of fluorescent labeling of the strut was discovered using the dye CY3. CY3 served as a hydrophobic tag for purification by hydrophobic interaction chromatography which enabled the separation of labeled and unlabeled species of S1 including a fraction …
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Characterization of natural organic matter and precursors to trihalomethanes using spectral flourescence signatures
… (SFS). The SFS is the total sum of emission intensity of a sample at different excitation wavelengths, recorded as a matrix of fluorescent intensity in coordinates of excitation and emission wavelengths. Among the six fractions, HPOA spectra were large and the peak intensity was also high. …
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The influence of fixation and cryopreservation of cerebrospinal fluid on antigen expression and cell percentages by flow cytometric analysis
… and compared across these methods. The median fluorescent intensity of select markers was also compared across these methods. Results: The majority of cell percentages were not statistically significantly different between Fresh and Cryopreserved CSF, and cell proportions were comparable. …
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Probing the distribution of gliadin proteins in dough and baked bread samples using conjugated quantum dots as a labeling tool
… conjugated to gliadin antibody and used as a fluorescent probe to track gliadin protein in dough and baked bread samples. The type and quantity of gluten proteins and their subfractions, gliadins and glutenins, are critical for baked product functionality. Gliadin proteins are also able to …
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Functional Polymers Containing Semi-Rigid Alternating Sequences
… were investigated. Extraordinarily high fluorescent intensity with excellent linearity was observed for the di-tert-butyl group-containing stilbene and maleic anhydride alternating copolymer in THF. We attributed the origin of the strong fluorescence to the “through space” π – π …
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Altering Nitric Oxide Bioavailability and Lipid Profiles in Endothelial Cells By Polycyclic Aromatic Hydrocarbons in Particulate Matter
… diacetate that reacts with NO to form a fluorescent triazolotluorescein. Then, lipids of HCAEC were extracted by chloroform and methanol and analyzed using 12T FT-ICR-MS to separate peaks.</p> <p>After PAH exposure, cell morphology noticeably changed, granularity increased, and viability …
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Mechanisms of dendritic peptide release
… ready for recycling or degradation. Immunofluorescent labelling was performed on hypothalamic slices of the transgenic rat where enhanced green fluorescent protein (eGFP) was tagged to vasopressin. Lysosomes were detected by the lysosome-associated membrane protein LAMP1. Correlation …
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Obesity-Induced Alterations in Sperm Quality, Testicular Morphology, and Extracellular Vesicle Localisation in Male Mice
… (H&E), immunohistochemistry (IHC) and immunofluorescent (IF) staining to investigate testicular morphology and assessment of EV localisation, respectively. EV-specific biomarkers such as CD63, CD9 and ANXA1 were targeted. Frozen testes were utilised for EV extraction and further western …
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Raman Spectroscopic Modeling Of T- Lymphocyte Activation And Detection Of Acute Renal Allograft Rejection
… kinetic studies designed to quantify the intensity and timing of cell surface receptor up-regulation. We found that there were significant RS signature differences between the MLR and non-activated (inactivated and resting) T lymphocytes while there was only a trend toward difference seen …
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Finite fraction method for tracking desinfection by-product precursors in water treatment
… of THM and HAA DBPs, respectively. Spectral Fluorescent Signature (SFS) is a process of subjecting each reasonably finite component (or fraction) of DOM to fluorescence scanning to produce unique spectral fluorescent characteristic of signature (hence the term SFS). SES method was …
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