Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"fluorescent antibody"”.
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Immune Blocking of Fluorescent Antibody Staining of Streptococcus Group A Compared with Anti-streptolysin O
<p>The fluorescent antibody blocking antibody (FABA) test has been discribed and compared with the conventional antistreptolysin 0 antibody (ASOA) test in the detection of Streptococcus pyogenes group A antibodies in sera samples.</p> <p>A slide inhibition test was used to detect the presence of …
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Detection of filamentous bacteria in activated sludge
… in Johannesburg, South Africa, in order to allow fluorescent antibody against these microorganisms to be produced and to facilitate and simplify their identification. Of the filaments only Nocardia could be isolated using standard techniques as the growth requirements of the other filaments …
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Identification, quantitation and immunogenicity of culture-derived soluble Babesia bigemina antigens
… (Quil-A) adjuvant, evoked primary and secondary antibody responses as detected and measured by the indirect fluorescent antibody (IFA) test and immunodiffusion tests.
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The prevalence of Legionella and mycoplasma seropositivity in the elderly in Cape Town
… was drawn. Laboratory methods: The indirect fluorescent antibody tests (Zeus Scientific Inc, New Jersey, USA) were used to detect the presence of antibodies to Mycoplasma pneumoniae and Legionella pneumophila. Results: The participation rate in this study was high, with 88,4% (677/766) taking …
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In Vitro Cultivation of Anaplasma Marginale
… and the organism was identified by the direct fluorescent antibody (FA) test and transmission electron microscopy. The PPE increased more than 3 times in the first experiment during a period of 14 days. Regression analysis of the data showed that the trend of growth was significantly (P = 0.05) …
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Efficiency of Monoclonal Antibodies to Detect Respiratory Virus Antigens in Clinical Specimens
… study were to determine the efficiency of immunofluorescent assay employing monoclonal antibodies from commercial sources to detect respiratory virus antigens in clinical specimens and to define the epidemiology of respiratory viruses in Bismarck, North Dakota.</p> <p>Throat swab, nasopharyngeal …
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ANALYSIS OF TUMOR SPECIFIC PROTEIN EXPRESSION IN GLIOBLASTOMA MULTIFORME (GBMs) TUMORS THROUGH IMMUNOHISTOCHEMISTRY
… from GBM tumors. Proteins were labeled with a fluorescent antibody and imaged with a confocal microscope. The tissue images were then analyzed using Imaris software. It was shown that there was a significant difference between the presence of the IDH1 mutation and Basgin, and also MGMT among …
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Comparison of Two Techniques for Enumeration of Human B and T Lymphocytes
… on ten of the twenty-one subjects and a fluorescent antibody stain was done on four subjects to confirm the EAC rosettes as B cells.</p> <p>There was a definite decrease in the number of monocytes after glass wool column separation. This led to a slight increase in the number of T cells …
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Detection of Bean Golden Mosaic Virus in Host Tissues and Infectivity of Its Genome in Bean Mesophyll Protoplasts
Indirect fluorescent antibody (F-Ab) staining, enzyme-linked immunosorbent assay (ELISA), and DNA-DNA hybridization on nitrocellulose (dot blot) were used to detect BGMV in P. vulgaris (Top Crop). F-Ab staining of sections from leaves, stems, and roots showed that viral antigen was present in a …
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A study of treponemal antigens
… T. minutum, and T. refringens was studied using fluorescent antibody staining and those organisms were found to contain a group of common antigens. The cross-reactivity was removed when the antisera was absorbed with Reiter cells. There was a second group of common antigens that were shared by …
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Comparison of Five Different Serological Assays for Determination of Antibody Levels Against Herpes Virus Type 1 and 2
… serological methods for measuring Herpes virus antibody. The five tests we compared are complement fixation test (CF), indirect immunofluorescent antibody assay (IFA), anticomplementary immunofluorescent assay (ACIF), enzyme linked immunoassay test (ELISA), and the latex agglutination test (LA). …
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As Evaluation of Methods Employed in the Detection of Pathogenic Escherichia Coli
… in addition to biochemical tests. More recently fluorescent methods have been utilized in identifying the pathogenic serotypes of E. coli. |The purpose of this thesis is to compare the methods of slide and tube agglutinations and fluorescent antibody studies in an effort to determine which …
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The Effects of Amphetamine Exposure on Neurodevelopment and Behavior in Early Life Stages of Danio Rerio
… interneural deficits are further supported with fluorescent antibody staining in the spinal cord, where inhibitory expression was significantly higher in the high amphetamine dose compared to controls. This study using 24 and 48 hpf zebrafish offers a novel perspective on early amphetamine …
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Laukinių ir naminių gyvūnų pasiutligės epidemiologija, diagnostika ir imunoprofilaktika Lietuvoje /
… proved that applying simultaneously the direct fluorescent antibody test (FAT) and rabies tissue culture infections test (RTCIT) methods it was possible to ensure a fast and effective determination of RV in the samples of wild and domestic animals under investigation thereby ensuring …
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Nociception and the Experience of Pain Signaling Due to Nerve Damage
… on the post synaptic level by comparing the fluorescent intensity mean (FIM) values of fluorescent antibody labeled NMDA receptors for glutamate and PSD95 clustering proteins for NMDAr at integration and motor synapses of spinal cord tissues. The FIM values were then correlated to the …
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Development of confirmatory testing strategies for suspect false PRRS-positive reactors in diagnostic serology
… positive results at varying rates. An indirect fluorescent antibody (IFA) test has been commonly employed to confirm or disprove the suspect false positive reactivity in the ELISA. However, the reliability of test results have been often questioned due to the subjectivity in determination of …
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Spore-forming bacteria as pharmaceutical factories
… first time, fragments of a humanized monoclonal antibody: kindly provided by Medimmune. It is possible to produce antibodies inside the spore core. This work proposes for the first time that such therapeutic spores might lack an outer coat, and demonstrates that antibody production continues. It …
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Vitamin A and glycoproteins of rat corneal epithelium
… conjunctival gland showed a strong fluorescent response to fluorescent antibody made against the intestinal glycopeptide affected by vitamin A.
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Ecological risk models for visceral leishmaniais [sic] in Bahia, Brazil and diagnosis of Trypanosoma cruzi infection in dogs in south central Louisiana
… were tested serologically using the indirect fluorescent antibody test (IFAT). Serum samples obtained from a total of 122 dogs from three kennels, and from client dogs from local veterinary practices tested by IFAT revealed a prevalence rate of 22.1%. Fifty randomly selected samples from this …
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Plant-based Multicomponent Vaccines against Enteric Diseases
… response to the plant synthesized antigens. Fluorescent antibody based flow cytometry analysis of immunized mouse spleen cells showed an increase in CD4+ T -helper cell populations. Following rotavirus challenge, passively immunized mouse pups showed a 50% reduction in diarrhea symptoms.</p> …
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