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Showing 1 to 20 of 261 for “"fluorescence spectroscopy"”.
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Applications of fluorescence spectroscopy to environmental chemistry
… is a photophysical study of the mechanism of fluorescence quenching by stable nitroxyl radicals, which are becoming an important analytical tool for the study of reactive transients in surface waters (1, 2). In part two, quenching of dissolved organic matter (DOM) fluorescence by nitroxides is …
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Point Spread Function Engineering in Fluorescence Spectroscopy
Die Synthese von Mikroskopie und Fluoreszenzspektroskopie hat das Mikroskop in ein vielseitiges Werkzeug zur funktionelle Analyse biologischer Systeme verwandelt. In dieser Arbeit wird untersucht, ob und wie kürzlich entwickelte Techniken zur Manipulation von Punktabbildungsfunktionen neue …
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Applications of fluorescence spectroscopy to environmental chemistry
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Earth, Atmospheric, and Planetary Sciences, 1992
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Acetylene--dispersed fluorescence spectroscopy and intramolecular dynamics
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1997.
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Characterising water treatment works performance using fluorescence spectroscopy
… efficiency. Here, the application of simple fluorescence excitation-emission (EEM) spectroscopy to OM characterisation in drinking water treatment was presented. Monthly raw and clarified water samples were obtained for 16 UK surface water treatment works. Fluorescence EEM spectroscopy was …
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Determination of Atrazine Concentration in Freshwaters Using Fluorescence Spectroscopy
… through liquid/gas chromatography or IR spectroscopy. However, these methods are either time-consuming or costly, so fluorescence spectroscopy is investigated as a more cost and time efficient alternative. Literature value of the optimal wavelength of atrazine was found to be 350 nm …
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Instrumental Refinements in Fluorescence Spectroscopy: Applications to Protein Systems
Made available in DSpace on 2014-12-14T04:27:44Z (GMT). No. of bitstreams: 1 7820975.pdf: 3573939 bytes, checksum: aafaf94c790bf911ac647d3c9f32d805 (MD5) Previous issue date: 1978
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Intermolecular interactions in supercritical fluid solutions from fluorescence spectroscopy
… a new technique to investigae SCF's using fluorescence spectroscopy to probe the local interactions. In contrast to thermodynamic measurements, this technique determines the strength of interactions on a molecular scale, which is important in the development of more accurate thermodynamic …
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Single molecule fluorescence spectroscopy of ClpXP-mediated substrate degradation
… occurs. Here, we present the single molecule fluorescence assay for probing the interaction between the ClpXP enzyme and its substrates. A covalently crosslinked ClpX hexamer maintain functionally stable form at the low concentration of single molecule level. Surface passivation through …
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Probing Structural Differences of Recombinant Prion Isoforms Using Fluorescence Spectroscopy
… the three isoforms using various tryptophan fluorescence techniques. For all six mutants, solvent accessibility was shown to decrease following conversion to the misfolded isoforms. Tryptophan accessibility data was used to evaluate two computational models of PrPSc, the beta-helix model and …
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Probing Structural Differences of Recombinant Prion Isoforms Using Fluorescence Spectroscopy
… the three isoforms using various tryptophan fluorescence techniques. For all six mutants, solvent accessibility was shown to decrease following conversion to the misfolded isoforms. Tryptophan accessibility data was used to evaluate two computational models of PrPSc, the beta-helix model and …
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Critical Evaluation of Some Atom Reservoirs for Atomic Fluorescence Spectroscopy
Made available in DSpace on 2014-12-13T20:10:23Z (GMT). No. of bitstreams: 1 7811264.pdf: 5569152 bytes, checksum: 5d4cdce5365b50ce194940b940bed844 (MD5) Previous issue date: 1978
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Considerations in Laser-Excited Atomic Fluorescence Spectroscopy and Related Investigations
Made available in DSpace on 2014-12-10T23:01:21Z (GMT). No. of bitstreams: 1 7219818.pdf: 8606797 bytes, checksum: 96af14b4b00ceec242cd9f4078522eb1 (MD5) Previous issue date: 1972
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Mechanical manipulation and visualization of single biomolecules using fluorescence spectroscopy
… this, we employed an integrated force-FRET (Fluorescence resonance energy transfer) assay to interrogate single biomolecules under applied forces between ~ 0.3 and 28 pN. In human cells, the ends of chromosomes are capped by 100-200 nt long telomeric DNA, consisting of tandem hexanucleotide …
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Development of new fluorescence spectroscopy approaches for the study of silica
… oxazole), has unique silica specific fluorescence and is used in biology to understand biosilicification. This ‘silicaphilic’ fluorescence is not well understood nor is the response to local environmental variables like solvent and pH. We investigated PDMPO in a range of environments: …
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Development & Application of Fluorescence Spectroscopy & Fluorescence Lifetime Imaging to Biology & Medicine
… (FRET) and development and application of fluorescence lifetime-resolved imaging microscopy (FLIM). It includes work to extend the FRET and FLIM methods for studying microsecond reaction kinetics. The common thread and unifying theme of the dissertation is the development and application of …
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Quantitative frequency-domain fluorescence spectroscopy in tissues and tissue-like media
… at lower cost, modern near-infrared optical spectroscopy offers the possibility of inexpensive technology for quantitative and non-invasive diagnoses. Hemoglobin is the dominant chromophore in the 700-900 nm spectral region and as such it allows for the optical assessment of hemoglobin …
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Non-invasive detection of oral cancer using reflectance and fluorescence spectroscopy
In vivo reflectance and fluorescence spectra were collected from patients with oral lesions, as well as healthy volunteers, in order to evaluate the potential of spectroscopy to serve as a non-invasive tool for the detection oral cancer. A total of 710 spectra were analyzed from 79 healthy …
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Application of fluorescence spectroscopy for the in-vial investigation of protein solutions
… protein formulation stability at all stages. Spectroscopy is a common laboratory tool for the characterisation of therapeutic proteins. Conventional techniques for determining protein denaturation are lab-based and require sample removal from the sealed primary packaging vial and sample …
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The Investigation of Model Biological Systems Under High Pressure by Fluorescence Spectroscopy
Fluorescence spectroscopy was used to investigate the effect of hydrostatic pressure on three systems: the binding of small ligands to polymers of (beta)-cyclodextrin, the interaction of ethidium bromide with phenylalanine-specific transfer ribonucleic acid from brewers yeast, and the pressure …
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