Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 118 for “"fluorescence resonance energy transfer"”.
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Fluorescence Resonance Energy Transfer (FRET) Based Sensors for Bioanalysis
… characterize new methods and sensors based on fluorescence resonance energy transfer (FRET) for bioanalysis. Chapter 3 describes the use of FRET between donor fluorophores and acceptor labeled murine macrophage cells. FRET microscopy was used to determine whether the donor molecules truly …
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Fluorescence Resonance Energy Transfer (FRET) systems for biomedical sensor applications
This thesis investigates the use of Fluorescence Resonance Energy Transfer (FRET) for biomedical sensor applications. FRET is a process by which energy is transferred, via long range dipole-dipole interactions, from a donor molecule (D) in an excited electronic state to an acceptor molecule (A). …
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Fluorescence resonance energy transfer-based biosensors for monitoring prostate specific antigen
… of localized regions of prostate tissue. Using fluorescence resonance energy transfer (FRET), several peptide substrates have been designed to respond to varying concentrations of PSA with a concomitant increase in fluorescence. In the near-infrared wavelength range, these fluorescent substrates …
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USING CONJUGATED POLYMERS AS BIOLOGICAL SENSOR BASED ON FLUORESCENCE RESONANCE ENERGY TRANSFER
… are to design novel molecular sensors based on fluorescence resonance energy transfer (FRET) between fluorophore (donor) and polydiacetylene (PDA, acceptor) for selective detection of biomolecules in solution. The work described in this dissertation is divided into three sections. In the first …
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Single-molecule fluorescence resonance energy transfer study of SNARE-mediated membrane fusion
… membrane fusion through single-molecule fluorescence resonance energy transfer (smFRET). Membrane fusion is one of the important cellular processes by which two initially distinct lipid bilayers merge their hydrophobic cores, resulting in one interconnected structure. For example, …
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An Investigation of the Phospholamban-Serca Regulatory Interaction with Fluorescence Resonance Energy Transfer
<p>With Fluorescence Resonance Energy Transfer (FRET), we are able to detect changes in the structure and affinity of the PLB-SERCA regulatory complex in live cells. Using this approach, we have detected a high level of PLB-SERCA interaction even at Ca2+ concentrations known to fully relieve PLB …
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Optimization and Applications of Fluorescence anisotropy assays and Fluorescence Resonance Energy Transfer Measurements
… mainly focuses on optimizing and applications of fluorescence anisotropy (FA) and FRET experiments for CaM-target interactions. First we evaluated the extent of interaction of fluorophores with CaM upon conjugation. In this study, three dyes were tested for influences of their charges on …
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Ratiometric copper(II) indicators based on poly(n-isoproprylacrylamide) and fluorescence resonance energy transfer
<p>An approach to ratiometric fluorescence detection of Cu(II) ions was devised by copolymerizing N-isopropylacrylamide with small percentages of bipyridine and amine monomers. The copolymer was divided into two portions. The amine group on one portion was functionalized with AlexaFluor555 (donor …
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Study Of Protein-Rna Interactions Using Fluorescence Resonance Energy Transfer (fret) And Single-Molecule Fret
… of RNPs are poorly understood. Single-molecule Fluorescence Resonance Energy Transfer (smFRET) techniques that provide information about heterogeneity and dynamic behaviors of molecules have been developed to investigate inter- and intra-molecular interactions. Here we have used FRET in …
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Visualization of cellular signaling by fluorescence resonance energy transfer in response to biochemical and mechanical microenvironments
… genetically-encoded biosensors that are based on fluorescence resonance energy transfer (FRET). FRET technology is a powerful tool to detect and visualize protein-protein interactions and enzymatic activities in living cells with high spatiotemporal resolution. In this thesis, I show that …
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Fluorescence resonance energy transfer-based subcellular visualization of FAK/Src activation by chemical and mechanical environment
… thesis, specific Src/FAK biosensors based on fluorescence resonance energy transfer (FRET) were developed and further targeted to subcompartments of plasma membrane to visualize local Src/FAK signaling events in live cells with high spatiotemporal resolution. Utilizing the subcellular-targeted …
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Development of a fluorescence resonance energy transfer optical nanoscale biosensor based on a liquid-core waveguide platform
… optical biosensor architecture utilizing fluorescence resonance energy transfer (FRET) is proposed. This biosensor architecture is then applied to a highly adept liquid-core waveguide platform. FRET is a distance-dependent signal transduction method that occurs between two fluorescent …
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Fluorescence resonance energy transfer study of the global folding of functional DNAs and electrohydrodynamic printing of protein arrays
Fluorescence resonance energy transfer (FRET) is used to monitor the metal ion dependent conformational change of the UO22+ specific DNAzyme 39E. Inactive metal ions, Mg2+ and Zn2+ are found capable of inducing folding between two pairs of stems while the active ions UO22+ and Pb2+ are not able to …
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Part A The application of fluorescence resonance energy transfer to reaction discovery Part B The stereodefined synthesis of tetrasubstituted olefins
… for enantioselective reaction monitoring using Fluorescence Resonance Energy Transfer (FRET) is described. The syntheses of enantiomeric epoxides bearing different fluorescent donors and a phenol that bore fluorescence acceptor were explored.* Part B describes the stereodefined synthesis of …
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INVESTIGATING PROTEIN - BILAYER COMPLEXES: A STUDY OF LIGAND - RECEPTOR INTERACTIONS AT MODEL MEMBRANE SURFACE BY USING ELECTRONIC ABSORPTION SPECTROSCOPY AND FLUORESCENCE RESONANCE ENERGY TRANSFER.
… scaffold. We also touch bases with major fluorescence techniques used in our experiments. Chapter 2 provides details on the preparation protocols of liposome and liposome-protein complexes. We confirmed protein-bilayer interactions by monitoring FRET between PDA and rhodamine molecules. …
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Structure and Dynamics of the Myosin Dimer Using Fluorescence Techniques
… called two-color FRET-FCS (a combination of Fluorescence Resonance Energy Transfer and Fluorescence Correlation Spectroscopy) and its application to the dynamical study of a model DNA hairpin. This technique can ultimately become useful in probing protein dynamics.
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Fluorescence characterization of protein and lipid dynamics
Fluorescence techniques were used to study the motion of protein domains in adenylate kinase, and to characterize the formation of myelin in neuron Schwann cell co-cultures. Domain motion upon substrate binding was examined in adenylate kinase using fluorescence resonance energy transfer. The …
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Redox Regulation of Cell Fate
… via electrical potentials; and second, a FRET (fluorescence resonance energy transfer)-based redox biosensor to monitor changes in the intracellular redox environment. Observations of altered intracellular redox status of Chinese hamster ovary (CHO) cells treated with electrical potentials …
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