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Showing 1 to 20 of 86 for “"fluorescence quenching"”.
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Determination of chlorine by fluorescence quenching
… for the determination of chlorine species. Fluorescence quenching to determine chlorine is a promising method because of its high sensitivity and selectivity. This study focuses on development of a sulfonamide-based collection system for low levels of chlorine in air. Quantitation of …
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Intramolecular Stacking Interactions Determined by Fluorescence Quenching
Made available in DSpace on 2014-12-13T20:09:52Z (GMT). No. of bitstreams: 1 7624093.pdf: 4061431 bytes, checksum: d0f51b1ad5ca0aecde8c3446dfc14c3a (MD5) Previous issue date: 1976
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Fluorescence Studies of Photosystem II Fluorescence Quenching During Desiccation
… In hydrated conditions nonphotochemical quenching (NPQ) mechanisms are able to safely dissipate excess excitation energy as heat, but mechanisms of NPQ associated with desiccation tolerance are still largely unclear. In the lichen Parmelia sulcata, photosystem protection has been …
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Mapping RNA Binding Surfaces on Hfq Using Tryptophan Fluorescence Quenching
… RNA sequences interact with Hfq, a tryptophan fluorescence quenching (TFQ) assay has been developed. Here it is presented that TFQ can correctly identify the binding face for two control sequences, A15 and U6, using the E. coli, S. aureus and L. monocytogenes Hfq homologues. Using fluorescence …
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A Kinetic and Thermodynamic Study of Exciplex Fluorescence Quenching by Ion Pairs
Tetraalkylammonium salts are shown to quench the fluorescence of the pyrene-N,N-dimethylaniline exciplex in solvents of low dielectric constant. Linear Stern-Volmer plots are obtained for quenching of both lifetime and intensity in dioxane. However, mechanistic complications are implied by …
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Towards the Understanding of Fluorescence Quenching Mechanisms : Molecular Dynamics Simulations of Dye-Quencher Interactions in Biomolecular Systems
… 6G and MR121) used in the development of fluorescence-based immunoassays for the detection of p53 antibodies. The "selective" fluorescence quenching property of tryptophan residues present in dye-conjugated peptide chains enables monitoring of conformational dynamics and antibody binding …
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THE DETERMINATION OF STABILITY CONSTANTS AND COMPLEXING CAPACITIES OF NATURAL ORGANIC MATTER FOR COPPER (2+), COBALT (2+) AND MANGANESE (2+) BY FLUORESCENCE QUENCHING TITRATION
… chelates still perform adequately.</p><p>A fluorescence titration technique is also described which can be used to determine complexing capacities (C(,L)) and 1:1 conditional stability constants (K) of fluorescing ligands with paramagnetic metal ions at the micromolar level. Titrations of …
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New sensor applications of poly(phenylene ethynylene)s
(cont.) Fluorescence quenching by these analytes was shown to be assisted by specific host-guest interactions involving the calix[4]arene macrocycles.
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INTERACTION OF POLYCYCLIC AROMATIC HYDROCARBONS WITH DISSOLVED AND SEDIMENT ASSOCIATED HUMIC MATERIALS (NMR, FLUORESCENCE)
<p>A fluorescence quenching method was developed for determining association constants of polycyclic aromatic hydrocarbons (PAH)s with dissolved and sediment bound humic materials. The technique is based upon the observation that PAH fluorescence in aqueous solution is quenched upon association …
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Fluorescent Tools for Watching Conformational Changes of Biological Molecules
Finally, we used a single molecule fluorescence quenching assay to study the timing of the neck linker conformational change that occurs as kinesin walks processively along a microtubule. We observed repetitive, alternating cycles of fluorescence and quenching as individual kinesin motors walked …
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Using Molecular Design to Influence Intermolecular Interactions
… Association constants were determined by fluorescence quenching experiments with fullerenes C70, C60, and Phenyl-C61-butyric acid methyl ester (PCBM). NMR titration experiments mimic fluorescence quenching results that suggest that association in solution increases with …
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Colloidal Gold as Molecular Capture Devices in Hybrid Nano/microfluidic Systems for Mass-Limited Sample Analysis
… of the gold colloid and the adsorbate in-device. Fluorescence microscopy was used to monitor fluorescence quenching during monolayer self-assembly of analytes on the colloidal gold surface. The kinetics of the heterogeneous reaction was fit using a second order reversible kinetics model and the …
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Immuntechnologische Verfahren zum Aufbau homogener Immunoassays sowie zur Selektion Antikörper produzierender Zellen
… Immunoassays zurück. Die Grundlage eines Fluorescence Quenching Immunoassays ist der gegenseitige Ausschluß zweier Antikörper bei der Bindung eines Dihapten-Konjugates. Das Konjugat besteht dabei aus dem Analyten und einem Fluorophor. Die beiden um die Konjugatbindung konkurrierenden …
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Design and Synthesis of Novel Cage-Functionalized Crown Ethers: A New Class of Ag Complexants.
… with Ag(I) have been studied. Atomic absorption, fluorescence quenching, and UV absorption have been used to study the interaction between the hosts (cage crown ethers) and guests (Ag+). For the cage-annulated crown ethers that contain aromatic rings, cation-π and π-π interactions may contribute …
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Influence of alloy metal nanoparticles on the fluorescence properties of a ruthenium complex
… of silver and gold (Ag/Au) nanoparticles on the fluorescence properties of the fluorescent dye, tris (2, 2’ – bipyridine) ruthenium (II) [Ru(bpy)<sub>3</sub>]<sup>+2</sup>. The dye showed fluorescence quenching by the gold, silver, and alloy nanoparticles. This extent of quenching, which is most …
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DESIGN AND CONSTRUCTION OF BIOSENSING PLATFORMS FOR THE DETECTION OF BIOMARKERS
… second one relays on a MoS2 nanosheet-mediated fluorescence quenching-restoration strategy; and the last one adopts a personal glucose meter as the signal readout through the conjugation of invertase with the substrate DNA of MTase. All of these established biosensors show good analytical …
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Optimization and Applications of Fluorescence anisotropy assays and Fluorescence Resonance Energy Transfer Measurements
… mainly focuses on optimizing and applications of fluorescence anisotropy (FA) and FRET experiments for CaM-target interactions. First we evaluated the extent of interaction of fluorophores with CaM upon conjugation. In this study, three dyes were tested for influences of their charges on …
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Kinetic Analysis of High Affinity Polyclonal Anti-Fluorescyl Immunoglobulin-G Antibodies
… rates were measured by following the increase in fluorescence quenching observed when anti-fluorescyl antibodies were added, in excess, to aqueous fluorescein. The reaction was made pseudo-first order to enable use of the same data processing routines for analysis of both association and …
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Fluorescence Depletion by Stimulated Emission in Single-Molecule Spectroscopy
… (STED) technique to the field of single-molecule fluorescence spectroscopy. It is demonstrated that fluorescence quenching induced by STED is reversible and can be repeated a large number of cycles on a single molecule. Being ideal point-like probes, single molecules can therefore be used to …
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