Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"fluorescence lifetime imaging microscopy (FLIM)"”.
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Computational Dead Time Correction in Fluorescence Lifetime Imaging Microscopy (FLIM)
<p>Fluorescence Lifetime Imaging Microscopy (FLIM) is a crucial tool in biomedical research. It can visualize and measure molecular interactions and cellular processes by analyzing the fluorescence decay of molecules in biological samples. Unlike traditional imaging methods based on fluorescence …
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Optimization of fluorescence lifetime imaging microscopy (FLIM) for studying the activity of enzymes in live cancer cells
… describes the process of optimizing a Fluorescence Lifetime Imaging Microscopy (FLIM) system in order to observe the dynamics of enzymes in live cancer cells. The enzyme studied throughout this research is Membrane Type 1 Matrix Metalloproteinase (MT1-MMP) which is a membrane-bound …
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Early selection of Chinese Hamster Ovary (CHO) cells using simultaneous label-free autofluorescence multi-harmonic (slam) microscopy and Fluorescence Lifetime Imaging Microscopy (FLIM) for biopharmaceutical cell line development
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2027-05-01
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Compressed fluorescence lifetime imaging microscope
Fluorescence lifetime imaging microscopy (FLIM) has been extensively applied in wide biomedical applications from single cell studies to medical diagnosis. While the state-of-the-art FLIM techniques are highly sensitive to biological dynamics such as fluorescence resonance emission transfer (FRET), …
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Towards The Development Of Intrinsically Fluorescent Unnatural Amino Acids For In Vivo Incorporation Into Proteins
… several previous biophysical studies involving fluorescence polarization (FP) and Förster resonance energy transfer (FRET) measurements, but to date was limited to solely E. coli expressed proteins. This thesis work will continue to explore Acd’s use in in vitro measurements from E. coli …
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Multi Scale Fluorescence Studies: The Spinning Disk Confocal Fluorescence Lifetime Imaging Microscopy &Amp; Single Molecule Nano-Metronome &Amp; Single Molecule Study of Tetramethyrhodamine-Dna Interaction
… The first part involves the frequency domain fluorescence lifetime imaging microscopy (FLIM) and an improvement of FLIM setup to decrease the out of focus background fluorescence without drastically sacrificing the data acquisition speed while taking fluorescence lifetime data. The setup …
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Graphene Microelectrode Arrays to Combine Electrophysiology with Fluorescence Imaging of Amyloid Proteins
… Two techniques used in amyloid research are fluorescence microscopy, to map the protein location and aggregation state, and electrophysiology, to examine the effect of the proteins on neurons. To enable these techniques to be combined, a transparent graphene microelectrode array (MEA) was …
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Advanced optical microscopy toolkits for non-invasive imaging in oncology
… stress in ex vivo human skin cultures by imaging endogenous and exogenous fluorescent compounds using two-photon excitation fluorescence (TPEF) and fluorescence lifetime imaging microscopy (FLIM); (2) visualizing water and lipid distribution as well as cellular morphology using coherent …
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Applying Flinet Deep Learning Model to Fluorescence Lifetime Imaging Microscopy for Lifetime Parameter Prediction
Fluorescence Lifetime Imaging Microscopy (FLIM) is essential in biomedical research, providing insights into cellular mechanisms and disease diagnosis by measuring the time molecules remain in an excited state before emitting a photon. Analyzing FLIM data is challenging due to the complex, …
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Study on gold nanoparticles for biological applications
… of biological research, especially in biological imaging and sensing due to their unique physical properties. Fluorescence is a highly-sensitive, non-invasive biological study method and has been widely used in a variety of research topics. The aim of this thesis is to study the unique optical …
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Techniques for high-speed high-resolution in-vivo volumetric fluorescence imaging
Optical microscopy has long been a cornerstone for exploring biological and biomedical systems. 3D microscopy with high spatialtemporal resolution over large fields of view is increasingly essential in life sciences. This thesis proposes two innovations to address this need: (1) high-resolution …
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Development of fluorescence lifetime imaging microscopy techniques for analysis of single extracellular vesicles
… This thesis presents the first use of two-photon fluorescence lifetime imaging microscopy (FLIM) to characterize the free and protein-bound reduced forms of nicotinamide adenine dinucleotide and nicotinamide adenine dinucleotide phosphate (NAD(P)H) content of EVs derived from cells, human urine, …
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Advancing fluorescence lifetime microscopy for quantitative bioimaging
This thesis presents the development of fluorescence lifetime imaging microscopy (FLIM) methodologies to advance biological understanding of neurodegeneration and stem cell differentiation. Two FLIM technologies enabling faster FLIM acquisition were developed and tested for high-throughput …
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Light in Living Systems: Ultra-Weak Photon Emission and Fluorescence Lifetime Imaging of Cellular Dynamics
… SPADs, confocal SPAD hybrids, EMCCD imaging, and PMT systems were implemented to test biological photon emission against mechanical artefacts. Entropy mapping, autocorrelation, and spectral analyses show that apparent low frequency rhythms are explained by system level artefacts (e.g. …
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Synthesis and evaluation of novel antivascular agents for two-photon activation
… 280 – 390 nM). Photochemical properties such as fluorescence lifetimes, fluorescence quantum yields, and two-photon absorption characteristics of the combretastatin derivatives were investigated spectroscopically. Fluorescence-lifetime imaging microscopy (FLIM) and light sheet fluorescence …
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Investigating the protein disaggregation machinery in the early secretory pathway
… closed and open conformation versions of a FRET-FLIM paired biosensor, which demonstrated its efficacy upon detection through Fluorescence Lifetime Imaging Microscopy (FLIM).
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Engineering Synthetic Biomolecular Condensates with Rationally-Designed Modular Repeat Proteins
… boundaries, and material properties. Multiple fluorescence-based methods—including fluorescence recovery after photobleaching (FRAP), fluorescence lifetime imaging microscopy (FLIM), fusion kinetic assays, and flicker spectroscopy—were employed to systematically characterise condensate dynamics …
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Multiplexed biochemical imaging reveals the extent and complexity of non-genetic heterogeneity in DNA damage-induced caspase dynamics.
… developed a unique sensing platform based on fluorescence lifetime imaging microscopy (FLIM) capable to multiplex at least three biosensors by utilizing Förster Resonance Energy Transfer (FRET) efficiently. After an overall introduction in Chapter 1, I describe the rational design and …
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Examining the Role of Phospholamban Phosphorylation on Interaction with SERCA Using Fluorescence Microscopy
… SERCA and PLB respectively, in HEK293 cells for fluorescence resonance energy transfer (FRET) microscopy experiments. We were able to use the native beta-adrenergic signaling system in the cells to control the state of PLB phosphorylation in a time-dependent manner. For the dissociation model to …
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Development of the single-molecule tracking and fluorescence lifetime techniques for biophysical measurements and biomedical applications
… 3D single-molecule tracking (3D-SMT) and lifetime measurement methods that can follow individual DNA molecules diffusing inside a mammalian cell and observe multiple annealing and melting events on the same molecules (Chapter 2). By comparing the hybridization kinetics of the same DNA …
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