Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 60 for “"fluorescence lifetime imaging"”.
-
Compressed fluorescence lifetime imaging microscope
Fluorescence lifetime imaging microscopy (FLIM) has been extensively applied in wide biomedical applications from single cell studies to medical diagnosis. While the state-of-the-art FLIM techniques are highly sensitive to biological dynamics such as fluorescence resonance emission transfer (FRET), …
-
LINEAR AND NONLINEAR FLUORESCENCE LIFETIME IMAGING FOR BIOSENSING APPLICATIONS
Fluorescence techniques, based on both linear and non-linear excitation, have been widely used in biosensing application. Among fluorescence techniques, Fluorescence Lifetime Imaging (FLIM) has gained increased importance because of their robust and versatile feature. FLIM is a technique to …
-
Computational Dead Time Correction in Fluorescence Lifetime Imaging Microscopy (FLIM)
<p>Fluorescence Lifetime Imaging Microscopy (FLIM) is a crucial tool in biomedical research. It can visualize and measure molecular interactions and cellular processes by analyzing the fluorescence decay of molecules in biological samples. Unlike traditional imaging methods based on fluorescence …
-
Advanced Fluorescence Lifetime Imaging and Spectroscopy Techniques for Biological Samples
… and chemical signalling. In this thesis, fluorescence-based studies were conducted on a fluorescent molecular rotor, BODIPY-Ci2, which was used to measure the intracellular viscosity. BODIPY-C12 is insensitive to the surrounding polarity using solvatochromic methods. It was demonstrated …
-
Development & Application of Fluorescence Spectroscopy & Fluorescence Lifetime Imaging to Biology & Medicine
… (FRET) and development and application of fluorescence lifetime-resolved imaging microscopy (FLIM). It includes work to extend the FRET and FLIM methods for studying microsecond reaction kinetics. The common thread and unifying theme of the dissertation is the development and application of …
-
The development of fluorescence lifetime imaging and an application in immunology
Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:35:59Z Item is restricted indefinitely.
-
Development of fluorescence lifetime imaging microscopy techniques for analysis of single extracellular vesicles
… This thesis presents the first use of two-photon fluorescence lifetime imaging microscopy (FLIM) to characterize the free and protein-bound reduced forms of nicotinamide adenine dinucleotide and nicotinamide adenine dinucleotide phosphate (NAD(P)H) content of EVs derived from cells, human urine, …
-
Applying Flinet Deep Learning Model to Fluorescence Lifetime Imaging Microscopy for Lifetime Parameter Prediction
Fluorescence Lifetime Imaging Microscopy (FLIM) is essential in biomedical research, providing insights into cellular mechanisms and disease diagnosis by measuring the time molecules remain in an excited state before emitting a photon. Analyzing FLIM data is challenging due to the complex, …
-
Development &Amp; Application of Fluorescence Spectroscopy &Amp; Fluorescence Lifetime Imaging to Biology &Amp; Medicine
… (FRET) and development and application of fluorescence lifetime-resolved imaging microscopy (FLIM). It includes work to extend the FRET and FLIM methods for studying microsecond reaction kinetics. The common thread and unifying theme of the dissertation is the development and application of …
-
Fast Fluorescence Lifetime Imaging Using a Full-Field Homodyne System with Applications in Biology
A fast, full-field fluorescence lifetime imaging system based on the homodyne technique was built and used for several applications. Also contained herein is the basic theory and background for fluorescence lifetime imaging, as well as data analysis of lifetime distributions. The fast, easy-to-use …
-
Fluorescence lifetime imaging microscopy to study protein aggregation in the context of neurodegenerative diseases
… presented in this thesis involves the use of fluorescence lifetime imaging microscopy (FLIM) and other biophysical techniques to elucidate the molecular mechanisms that underlie different amyloid-associated neurodegenerative dis- eases. Currently, there is no prevailing therapeutic option for …
-
Light in Living Systems: Ultra-Weak Photon Emission and Fluorescence Lifetime Imaging of Cellular Dynamics
… SPADs, confocal SPAD hybrids, EMCCD imaging, and PMT systems were implemented to test biological photon emission against mechanical artefacts. Entropy mapping, autocorrelation, and spectral analyses show that apparent low frequency rhythms are explained by system level artefacts (e.g. …
-
Determining S6K1 localisation and interactions with mTORC1 in live cells using fluorescence lifetime imaging microscopy
… and adiposity. Using GFP technology and advanced imaging, localisation of S6K1 has been established (47% nucleus and 53% cytoplasm). S6K1 strongly (Δτ=200 ps) interacts with the complex scaffold protein, raptor, and when the presence of the latter protein is increased, S6K1 translocates to the …
-
Optimization of fluorescence lifetime imaging microscopy (FLIM) for studying the activity of enzymes in live cancer cells
… describes the process of optimizing a Fluorescence Lifetime Imaging Microscopy (FLIM) system in order to observe the dynamics of enzymes in live cancer cells. The enzyme studied throughout this research is Membrane Type 1 Matrix Metalloproteinase (MT1-MMP) which is a membrane-bound …
-
Multi Scale Fluorescence Studies: The Spinning Disk Confocal Fluorescence Lifetime Imaging Microscopy & Single Molecule Nano-Metronome & Single Molecule Study of Tetramethyrhodamine -Dna Interaction
… absence of any biological activities of DNA, the fluorescence emission of TMR undergoes multiple slow transitions among at least four different fluorescence states. While the actual number of fluorescence states and therefore the actual rates of transition among them remains open for discussion …
-
Multi Scale Fluorescence Studies: The Spinning Disk Confocal Fluorescence Lifetime Imaging Microscopy &Amp; Single Molecule Nano-Metronome &Amp; Single Molecule Study of Tetramethyrhodamine-Dna Interaction
… The first part involves the frequency domain fluorescence lifetime imaging microscopy (FLIM) and an improvement of FLIM setup to decrease the out of focus background fluorescence without drastically sacrificing the data acquisition speed while taking fluorescence lifetime data. The setup …
-
Early selection of Chinese Hamster Ovary (CHO) cells using simultaneous label-free autofluorescence multi-harmonic (slam) microscopy and Fluorescence Lifetime Imaging Microscopy (FLIM) for biopharmaceutical cell line development
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2027-05-01
-
Development of Methods for Structure and Function Determination in Living and Fixated Cells on the Single-Molecule Level Based on Coincidence Analysis and Spectrally-Resolved Fluorescence Lifetime Imaging Microscopy [SFLIM]
… For this purpose an algorithm based on confocal imaging microscopy has been developed, which facilitates the separation of colocalized dyes by their fluorescence lifetimes and spectral characteristics. The accuracy and applicability of the method was in this work using biological calibration …
-
Time Resolved Light Sheet Microscopy
… Time-correlated single photon counting fluorescence lifetime imaging microscopy is just one of the tools used to carry out this line of research. Here we demonstrate a direct interaction between two proteins involved in gene regulation and expression; p21 and FMN2. Furthermore, we also …
-
Functional Imaging Through Dark State Dynamics
… triplet state, was developed and called FAPA, Fluorescence Anomalous Phase Advance. This technique is a sensitive and fast reporter of dark state dynamics and can be used for imaging. In addition, a genetically-encoded microviscosity and micro-environmental mapping ability with high …
Page 1 of 3