Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 85 for “"fluorescence in situ hybridization"”.
-
An automated multicolour fluorescence in situ hybridization workstation for the identification of clonally related cells
The methods presented in this study are aimed at the identification of subpopulations (clones) of genetically similar cells within tissue samples through measurement of loci-specific Fluorescence in-situ hybridization (FISH) spot signals for each nucleus and analyzing cell spatial distributions by …
-
Fluorescence in Situ Hybridization Analysis of Human Embryos Derived From in Vitro and in Vivo Matured Oocytes
… oocytes collected for the purpose of in vitro fertilization and embryo transfer display several levels of nuclear maturity. Preovulatory or mature oocytes, technically those that are Metaphase I or II, are inseminated shortly after aspiration and assessed for fertilization the …
-
Establishment and implementation of multiplexed error robust fluorescence in situ hybridization to study spatial mRNA expression in cells and tissues
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-12-01
-
Identification of chemoautotrophic microorganisms from a diffuse flow hydrothermal vent at EPR 9° North using 13C DNA Stable Isotope Probing and Catalyzed Activated Reporter Deposition-Fluorescence in situ Hybridization
… levels. To better understand these underlying processes and the organisms catalyzing them, this research used DNA Stable Isotope Probing (SIP) combined with Catalyzed Activated Reporter Deposition‐Fluorescence in situ Hybridization (CARD‐FISH) to identify the microorganisms …
-
Identification of chemoautotrophic microorganisms from a diffuse flow hydrothermal vent at EPR 9° north using ¹³C DNA stable isotope probing and catalyzed activated reporter deposition-fluorescence in situ hybridization
… levels. To better understand these underlying processes and the organisms catalyzing them, this research used DNA Stable Isotope Probing (SIP) combined with Catalyzed Activated Reporter Deposition-Fluorescence in situ Hybridization (CARD-FISH) to identify the microorganisms …
-
Generalist 3D Cell Phenotyping for All-Type Tissues
Tissue-clearing methods, light-sheet microscopy, and antibody labeling enable extracting cellular and subcellular information, producing large amount of image data needs to be analyzed. Hundreds of heterogeneous cell types were detected through the data obtained across species and types of tissues. …
-
From coarse fate choice to precise pattern: post-mitotic progenitor targeting
… these progenitors migrate across the animal remains unclear. Irradiation experiments eliminate all neoblasts outside shielded areas, allowing for the visualization of cell migration from the remaining neoblasts, but irradiated animals may not reflect homeostatic progenitor migration patterns. To …
-
Oligonucleotide Design for Whole Genome Tiling Arrays
Oligonucleotides are short, single-stranded fragments of DNA or RNA, designed to readily bind with a unique part in the target sequence. They have many important applications including PCR (polymerase chain reaction) amplification, microarrays, or FISH (fluorescence in situ hybridization) probes. …
-
Simultaneous partial Nitritation, Anammox and Denitrification (SNAD) process for treating ammonium-rich wastewaters
… received a great deal of attention during the last years. Since the discovery of anammox bacteria the traditional theory of biological nitrogen removal has been extended to new nitrogen metabolic pathways. This thesis deals with the study of Simultaneous partial Nitritation, Anammox and …
-
Molecular Signatures of gastric cancer: An integrated approach to molecular cytogenetics, whole genome copy number and transcriptome profiles
Signature chromosomal translocations in gastric cancer are poorly described. By integrating spectral karyotype and high-resolution genome copy number data, 45 recurrent translocations in 17 human gastric cancer cell lines were identified. Dissecting a simple rearrangement in SNU-1 cells using …
-
Characterization of genetically distinct neuronal subtypes in the lateral habenula using Cre mouse lines
The lateral habenula (LHb) is a bilateral brain structure implicated in action selection, reward processing, and motivation. The LHb projects to the ventral tegmental area (VTA) and dorsal raphe nucleus (DNR), and is thus involved in both dopaminergic and serotonergic pathways. Recent single-cell …
-
Pressure Treatment of Polytene Chromosomes and Computer Vision Techniques for High Resolution Cytogenetic Studies
The exceptional cytology provided by the interphase chromatin organization in polytene chromosomes has made Drosophila the premier model organism for analysis of chromosome structure and behavior. A major impediment to full exploitation of polytene chromosome cytology has been the difficulty in …
-
Trojan horses in the marine realm: characterizing protistan parasite ecology in coastal waters
… diverse drivers of energy and nutrient flow in the marine environment, with recent research suggesting significant roles in global carbon cycling throughout the water column. Top-down controls on planktonic protists include grazing and parasitism, processes that both contribute to nutrient …
-
Trojan Horses in the Marine Realm: Characterizing Protistan Parasite Ecology in Coastal Waters
… diverse drivers of energy and nutrient flow in the marine environment, with recent research suggesting significant roles in global carbon cycling throughout the water column. Top-down controls on planktonic protists include grazing and parasitism, processes that both contribute to nutrient …
-
Single molecule investigation of antiviral signaling
Retinoic acid-inducible gene 1 (RIG-I) plays a major role in antiviral innate immunity by detecting cytoplasmic viral RNA and triggering the pathway that leads to transcriptional activation of type I interferon (IFN). Type I IFN induces a large set of genes called interferon-stimulated genes (ISGs) …
-
THE EFFECTS OF AGE AND HETEROCHROMATIN ON FREQUENCIES OF ACQUIRED CHROMOSOMAL ANEUPLOIDY IN UNCULTURED HUMAN LEUKOCYTES
… relationship between autosomal loss and age remains unclear. The emergence of the specific and highly sensitive fluorescence in situ hybridization (FISH) technology has enabled investigators to study interphase cells, thereby overcoming problems inherent with the study of metaphase spreads for …
-
Caracterización clínica de pacientes pediátricos con síndrome de deleción 22q11 atendidos en Hospital de Bogotá, durante el periodo de 2010 a 2020
… la segunda causa de cardiopatía congénita en la infancia (1). El 90-95% de los casos se deben a microdeleciones de novo, con un riesgo de recurrencia de ∼1%. El 22q11DS afecta múltiples sistemas que incluyen el sistema cardiaco e inmunológico, alteraciones faciales y dificultades en el …
-
Processes and community structure in microbial biofilms of the River Elbe: relation to nutrient dynamics and particulate organic matter
The conceptual subject of this study was to investigate the effects of microbial biofilms of the hyporheic zone of the river Elbe on nutrient dynamics and elimination. This resulted in four aspects, which were carried out separately. One aspect was the investigation of the dynamics of inorganic …
-
Greining erfðamengja xTriticoleymus og Rosa L.
Melhveiti (xTriticoleymus) er blendingur hveiti (Triticum) og melgresis (Leymus) tegunda. Erfðamengi xTriticoleymus er áhugavert vegna þess að blendingurinn er frjór og mögulega hægt að rækta hann sem nytjaplöntu. Í þessari rannsókn voru litningar einangraðir úr rótarendum xTriticoleymus og …
Page 1 of 5