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Showing 1 to 12 of 12 for “"fluorescence fluctuation"”.
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Analysis and Applications of Fluorescence Fluctuation Spectroscopy
… the influence of the molecular brightness on the fluctuation amplitude G(0). This dependence allows to resolve multiple species based on the difference in their molecular brightness, which we applied to a titration study of an antibody with its ligand. The global fit of the average photon counts …
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Studying viral-like particles using fluorescence fluctuation spectroscopy
… viral assembly, but it is notoriously difficult. Fluorescence fluctuation spectroscopy (FFS) is a promising experimental technique that determines the interactions of proteins and other biomolecules from fluorescence signal fluctuations with single molecule sensitivity. FFS can directly quantify …
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Axial-scan fluorescence fluctuation spectroscopy: initial development and experimental challenges.
Summary abstract not available.
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Fluorescence fluctuation spectroscopy and microscopy : application to cellular molecular dynamics and organization
Fluorescence fluctuation spectroscopy and microscopy have been powerful tools for studying molecular organization and dynamics in cells. Fluorescence Correlation Spectroscopy (FCS) has been widely applied to probing molecular dynamics in live cells with single molecule sensitivity and the ability …
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Quantitative Fluorescence Studies in Living Cells: Extending Fluorescence Fluctuation Spectroscopy to Peripheral Membrane Proteins
… aims at addressing this challenge by extending fluorescence fluctuation spectroscopy (FFS) to simultaneously measure the oligomeric state of peripheral membrane proteins in the cytoplasm and at the plasma membrane. We developed a new method based on z-scan FFS that accounts for the fluorescence …
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Advancing Fluorescence Fluctuation Microscopy in Living Cells: From Non-stationary Signals to Ternary Protein Interactions
Fluorescence fluctuation spectroscopy (FFS) is a powerful method for quantifying protein interactions. By exploiting the brightness of fluorescence intensity fluctuations we are able to measure the stoichiometry of protein complexes. FFS is particularly valuable because it allows real-time …
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Fluorescence Depletion by Stimulated Emission in Single-Molecule Spectroscopy
… (STED) technique to the field of single-molecule fluorescence spectroscopy. It is demonstrated that fluorescence quenching induced by STED is reversible and can be repeated a large number of cycles on a single molecule. Being ideal point-like probes, single molecules can therefore be used to …
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Fluorescence Detectors for Proteins and Toxic Heavy Metals
… based on two-photon excitation intrinsic protein fluorescence using a visible 532 nm diode-pumped nano laser as the excitation source. Proteins that exhibit intrinsic fluorescence must contain at least one tryptophan, tyrosine, or phenylalanine residue in their amino acid sequences. The detector …
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Scalable Chemical Tool for Analyzing Brain: Electron Microscope-Like Images with Fluorescent Microscope
… dense labeling of membranous structures while fluorescence microscopy (FM) allowed neuroscientists to identify and study specific biomolecules of interest. However, it would be ideal if such imaging could be achieved with FM (i.e., conventional light microscope), allowing anyone to identify and …
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Printing methodologies for functional bioarrays
… is obtained by employing the advanced fluorescence fluctuation technique Raster Image Correlation Spectroscopy, here employed to probe protein-ligand interactions in artificial aqueous droplets by mapping concentration and diffusion coefficients of fluorolabeled ligands at nanomolar …
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Resonance Fluorescence in a Photonic Crystal
… problems, that are related to the full resonance fluorescence problem: a pure dephasing model, the driven atom and resonance fluorescence in free space. In the lowest order of the system-environment coupling, the one-time expectation values for the full problem are calculated analytically and the …