Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 36 for “"fluorescence enhancement"”.
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Fluorescence Enhancement Strategies for Polymer Semiconductors
… pumped laser diodes. This thesis investigates fluorescence enhancement strategies for high-mobility polymer semiconductor systems and the mechanisms by which they currently display poor emission properties. Four independent approaches were taken and are detailed as follows. 1. Solubilising …
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Harnessing the optical properties of gold nanorods: fluorescence enhancement, biosensing and photothermal therapy
… plasmon resonance (LSPR) can result in the enhancement of many photophysical processes such as light absorption, Rayleigh (Mie) scattering, and fluorescence. The presence of a strong electromagnetic field has been observed to enhance spectroscopic molecular signals from fluorophores, …
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Characterization of protein induced fluorescence enhancement (PIFE): A label-free protein assay with short distance sensitivity
… In this report, we report on a newly developed fluorescence assay termed Protein Induced-Fluorescence Enhancement (PIFE), which circumvents protein labeling and displays sharp detection sensitivity in short distance regions, overcoming the inherent limitations of FRET. The enhancement of …
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Next generation instrumentation for photonic crystal biosensors: a passage to early detection of cancer
… approach done using photonic crystal enhanced fluorescence (PCEF) instrumentation as the best way to achieve this goal. We introduce a model for predicting enhanced fluorescence (EF) performance of a photonic crystal (PC) in the context of a given set of instrument parameters as well as …
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Sensitive microarray-based biomarker detection using photonic crystal enhanced fluorescence and label-free imaging
Fluorescence-based biomarker detection in serum, saliva, urine, or breath condensate samples holds great promise for disease diagnostics and therapy monitoring because of its non-invasive nature. The microarray format, for its high-throughput and low analyte consumption characteristics, is …
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Self-Assembly of a Bio-Driven Single Molecule DNA Sequencer
… correct sequence by the enzyme DNA polymerase. Fluorescence enhancement is achieved by the use of a plasmonic nanoantenna. Chapter 1 provides an overview of existing state of the art technologies and underlying theory behind plasmonic nanoantenna sequencing. Chapter 2 provides the materials and …
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Biosensor and assay development for the detection of disease biomarkers at the point of care
… crystal biosensor platform with the capacity for fluorescence enhancement was combined with an automated multiplexed microfluidic flow system and an automated spot-finding and data generation algorithm for the detection of a cancer biomarker in human serum and plasma.
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Surface plasmon enhanced fluorescence for biological imaging : from visible to short-wave infrared
Fluorescence imaging offers high spatio-temporal resolution, low radiation dosage exposure, and low cost among all the available imaging modalities, for example, magnetic resonance imaging, computerized tomography and positron emission tomography. Imaging probes of high emissivity and …
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Silicon based photonic crystal coupled to an optical cavity for highly sensitive biodetection
… by Sir John Frederick William Herschel in 1845, fluorescence detection technology has developed extensively to be used in disease diagnosis, DNA sequencing, cellular imaging, flow cytometry, forensics, and monitoring processes in biological systems. In order to achieve better sensitivity and …
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Interactions of ATP, ADP and magnesium ion with rubisco activase and their effects on rubisco activation
… induced a transient increase in the tryptophan fluorescence of spinach rubisco activase. The kinetics of the fluorescence change was similar to that of ATP hydrolysis. ADP was a competitive inhibitor of the ATP induced fluorescence enhancement. This complex exhibited a higher molecular size …
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Photophysics of Biological and Synthetic Multichromophoric Systems : Spectroscopic Investigations of Bacterial Light-Harvesting Complexes and of Carbonyl-Bridged Triarylamine Derivatives
… RC-LH1 from Rhodopseudomonas palustris. Their fluorescence decay was recorded dependent on the excitation fluence and the repetition rate of the excitation laser. Both parameters were varied over three orders of magnitude. We observed three components in the decays with characteristic decay …
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Improved microarray sensitivity using photonic crystal enhanced fluorescence
… the proliferation of research on modifying fluorescence with optical resonances, there have been few evaluations of the practical impact of enhanced fluorescence on high-throughput biological assays such as microrarrays. This work details the application of photonic crystal (PC) surfaces as …
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Applications of Molecular Switching and Logic
… and PASS 1 with H+ input, 700 nm near-infrared fluorescence output and 615 nm red light excitation. The gates carry an azaBODIPY (azaborodipyrromethene) fluorophore and an aliphatic tertiary amine as the H+ receptor where necessary. The H+-induced fluorescence enhancement factors are signatures …
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Unveiling Dna Polymerase Synthesis And Proofreading Activities - One Molecule At A Time
… Second, we applied a new single molecule fluorescence technique, single molecule protein induced fluorescence enhancement (smPIFE), to our system to accurately measure binding dynamics and to strengthen our interpretation of the polymerase position on the DNA. Third, determined how …
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Single molecule pull down of double strand RNA binding proteins
… probed by single molecule protein induced fluorescence enhancement (smPIFE), which demonstrates how single molecule approaches could be utilized to provide new insight into molecular mechanisms involved in protein-RNA interaction. Collectively, our study highlights the diverse nature of …
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Optical Characterization and Evaluation of Dye-Nanoparticle Interactions
… the metal, observations of quenching as well as fluorescence enhancement have been reported. Based on the phenomenon of surface plasmon resonance as well as the properties of metallic nanoparticles, this research reports the interaction of gold and silver nanoparticles in an aqueous dye solution. …
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DEVELOPMENT OF A NEW CHIRAL MONOLITHIC CAPILLARY COLUMN AND A FLUORESCENCE SPECTROSCOPIC STUDY OF A SELECTIVE OFF-ON PET SENSOR FOR THE DETECTION OF ZINC IONS
… found to respond selectively to Zn2+ ions with fluorescence enhancement. The fluorescence properties and binding response were evaluated in the presence of water and a Lewis base, which we found to have a marked effect on the fluorescence signal. The selectivity of the sensor for Zn2+ was also …
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Guided -Mode Resonance in Planar Photonic Crystals: Application to Sensing
… PCs are also studied for application as fluorescence enhancement biosensors. For the first time, a PC capable of a dual enhancement modality (enhanced excitation and enhanced extraction of fluorescence) is demonstrated for boosting quantum dot fluorescence by over two orders of magnitude. …
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Optical Properties of Individual Nano-Sized Gold Particle Pairs
… To attain a figure of merit, the intensity of fluorescence light from dye molecules in the surrounding medium was measured at various directions of polarisation. The field enhancement was turned into fluorescence enhancement, even providing a means for sensing the presence of very small MNPs of …
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Using photo-instability to quantify fluorophores and achieve super-resolution imaging
… fluorophores and count the stepwise drops in fluorescence intensity to determine the number of ligand binding sites. Results from single α7 and neuromuscular junction nicotinic acetylcholine receptors in mammalian cell membranes are shown. The results indicate that there are two bungarotoxin …
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