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Showing 1 to 7 of 7 for “"fluorescence depolarization"”.
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Dynamics of biological macromolecules investigated by fluorescence depolarization
… to be important to their physiological function. Fluorescence depolarization is a powerful technique for the investigation of these dynamics. A general overview of the use of time-resolved fluorescence to investigate nanosecond dynamics is presented. The basic theory and experimental techniques …
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Dynamics of biological macromolecules investigated by fluorescence depolarization
… to be important to their physiological function. Fluorescence depolarization is a powerful technique for the investigation of these dynamics. A general overview of the use of time-resolved fluorescence to investigate nanosecond dynamics is presented. The basic theory and experimental techniques …
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Fluorescence Depolarization Study of Internal Tryptophan Mobility in Hydrated Azurin Films
… dynamics was investigated using steady state fluorescence depolarization on azurin, which has a single tryptophan residue well-buried in the hydrophobic interior. Azurin was imbedded in a thin, solid, water-permeable polymer film. This procedure inhibited whole-protein motion while allowing …
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The Influence of Incorporated Cholesterol Auto-Oxides Upon The Permeability and Fluidity of Multilamellar Vesicles (Oxidized Sterols)
… scanning calorimetry and steady state fluorescence depolarization. Differential scanning calorimetry on dipalmitoyl lecithin:sterol dispersion of the B ring sterol oxides, 7-ketocholesterol and cholestan-triol, indicated that they were similar to cholesterol dispersions in their ability …
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Fluorenscence depolarization study of internal tryptophan mobility in hydrated azurin films
… dynamics was investigated using steady state fluorescence depolarization on azurin, which has a single tryptophan residue well-buried in the hydrophobic interior. Azurin was imbedded in a thin, solid, water-permeable polymer film. This procedure inhibited whole-protein motion while allowing …
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The effects of altered membrane fatty acid composition on the toxic interactions of heavy metals with Saccharomyces Cerevisiae
… in plasma membrane lipid order, detected by fluorescence depolarization measurements. Levels of the lipid peroxidation products thiobarbituric acid-reactive substances (TBARS) and conjugated dienes were markedly higher in PUF A-enriched cells, compared with unsupplemented cells, following …
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Insights into the Mechanisms of Metal Oxide and Cationic Antimicrobials
… taken for individual cells to permeabilize using fluorescence microscopy. We found a time lag of 5–10 minutes between adsorption and death and found that the time-to-die of an individual cell was well correlated with the rate of adsorption. We also found that the time-to-die and equilibrium …