Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"fluorescence background"”.
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Oxygen stable isotopic analysis of calcite by Raman microprobe spectrometry
… nitrogen temperatures (78-90K) to remove the background from Mn²⁺ fluorescence and minimize the overlap of the symmetric stretching bands. At near liquid nitrogen temperatures, the presence of the C¹⁸O¹⁶O₂²⁻, C¹⁷O¹⁶O₂²⁻, and C¹⁶O₃²⁻ symmetric stretching bands are clearly visible. Initially, …
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Quantitative mRNA detection with advanced nonlinear microscopy
… to detect mRNA without the interference of fluorescence background. The thesis presents the fundamental study on the probes used in SHaSM, the concept and instrumental layout of the two technologies, and the detection as well as quantification of mRNA transcript in cells and tissues by super …
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Advanced Fluorescence Correlation Techniques to Study Membrane Dynamics
Fluorescence Correlation Spectroscopy (FCS) is a powerful tool to measure important physical quantities such as concentrations, diffusion coefficients, diffusion modes or binding parameters, both in solution and in membranes. However, it can suffer from severe artifacts, especially in non-ideal …
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Single-Objective Tilted Light Sheet Illumination with Exchange-PAINT and Deep Learning for Fast, Accurate, and Precise 3D Single-Molecule Super-Resolution Imaging in Mammalian Cells
Single-molecule super-resolution fluorescence microscopy is a powerful method for imaging detailed biological structures at the nanoscale. However, imaging capabilities in thick samples such as mammalian cells remain limited due to increased fluorescence background which degrades the achievable …
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Resonant soft X-ray studies of charge orders in high-temperature cuprates with energy-resolving transition edge sensors
… example Cu in cuprates; however, the inelastic fluorescence (usually referred as background in RSXS experiments) is also the most intense at resonance making short-ranged charge orders harder to be defined. The fluorescence background can only be separated by introducing energy-resolving …
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Screening of algal cells using microdroplet technology
… cell suspension prior to the encapsulation. A fluorescence-activated droplet sorting platform was used to detect the fluorescence of encapsulated algal cells. Following modifications to the optics of a previously used platform, the fluorescence measurement uncertainty was reduced from 87% to …
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Studying viral-like particles using fluorescence fluctuation spectroscopy
… viral assembly, but it is notoriously difficult. Fluorescence fluctuation spectroscopy (FFS) is a promising experimental technique that determines the interactions of proteins and other biomolecules from fluorescence signal fluctuations with single molecule sensitivity. FFS can directly quantify …
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Advanced Raman spectroscopy for bioprocess monitoring
… so far has been the disturbance of the broad fluorescence background especially in biological samples. The main objective of this thesis was to find solutions for increasing the limit of detection (LOD) for biomolecules, being capable to detect them during the course of the process reliably …
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DNA origami substrates as a versatile tool for surface-enhanced Raman scattering (SERS)
… analogue non-covered SERS substrates. First, the fluorescence background of dye molecules that are located in between the AuNP surface and SLG is efficiently reduced. Second, the photobleaching rate of the incorporated dye molecules is decreased up to one order of magnitude. Third, due to the …
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New Developments On High-resolution Luminescence Spectroscopy And Their Application To The Direct Analysis Of Organic Pollutants
… namely Shpol'skii Spectroscopy (SS) and Fluorescence Line Narrowing Spectroscopy (FLNS). Although these two techniques have long been recognized for their capability in providing direct determination of target PACs in complex environmental samples, several reasons have hampered their …