Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 46 for “"fluorescence anisotropy"”.
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Real time PCR measurement by fluorescence anisotropy
… real-time PCR that utilizes the fluorescent anisotropy (FA) of labeled primers. FA, when measured at constant temperature, is indicative of the molecular mass to which the fluorophore is attached. Specificity is improved with the FA method over the use of intercalating dyes since the …
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Optimization and Applications of Fluorescence anisotropy assays and Fluorescence Resonance Energy Transfer Measurements
… mainly focuses on optimizing and applications of fluorescence anisotropy (FA) and FRET experiments for CaM-target interactions. First we evaluated the extent of interaction of fluorophores with CaM upon conjugation. In this study, three dyes were tested for influences of their charges on …
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Theoretical and lab based studies of fluorescence anisotropy toward the analysis of multiple homo-FRET pairs
… receptor proteins because homo-FRET causes the fluorescence anisotropy of the system to decrease when the number of identical fluorophores within energy transfer distance increases. Theories that describe these systems apply an assumption of equal fluorescence efficiency for all sites [1, 2], …
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Development and Application of a Fluorescence Anisotropy Microplate Assay for Analyzing the Interaction of Steroid Hormone Receptors With DNA and Coactivators
… microplates to develop the simple and rapid fluorescence anisotropy microplate assay (FAMA). The FAMA was applied to the binding of estrogen and progesterone receptors to their respective DNA response elements. The FAMA is also the first system suitable for screening large compound libraries …
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DEVELOPMENT OF A NEW SCREENING AND DETECTION METHOD FOR IDENTIFYING PROTEIN-SMALL MOLECULE INTERACTIONS
… technique termed Dynamic Isoelectric focusing Anisotropy Binding Ligand Assay (DIABLA) is being in collaboration with the Tolley Laboratory (SIU) developed to fulfill this task. In this technique, a protein mixture is separated within the capillary using dynamic isoelectric focusing, while a …
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Local Viscosity and Environment on the Nanometer Scale
… setup for two photon excitation time-resolved fluorescence anisotropy and lifetime measurements was built and used to understand the local environment and viscosity. The rotational correlation time constants by the fluorescence anisotropy provide the information on the hydrodynamic volume and …
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Steady-State and Ultrafast Fluorescence Depolarisation in Rigid-Rod Conjugated Polymers
… underlying physics of steady-state and ultrafast fluorescence depolarisation in conjugated polymers. Depolarisation is due to fluorescence anisotropy: the angular difference between the absorption and emission transition dipole moments of a molecule. Polarised spectroscopy results from a polymer …
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Synthesis and Physical Properties of Helical Nanosized Quinoline-based Foldamers: Structure, dynamics and photoinduced electron transport
… state of foldamers in gas phase; DOSY and fluorescence anisotropy assessed the diffusion (translational and rotational, respectively) of foldamers in solution. All of these techniques revealed that quinoline-based foldamers are rigid and that helical conformation is conserved. Photoinduced …
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Advanced Fluorescence Lifetime Imaging and Spectroscopy Techniques for Biological Samples
… and chemical signalling. In this thesis, fluorescence-based studies were conducted on a fluorescent molecular rotor, BODIPY-Ci2, which was used to measure the intracellular viscosity. BODIPY-C12 is insensitive to the surrounding polarity using solvatochromic methods. It was demonstrated …
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Lead compound discovery for myotonic dystrophy
… compounds and the development of a fluorescence anisotropy assay for a high-throughput screening of the NCI Diversity Set III compound library. From such studies I was able to identify several lead compounds that are successful at inhibiting the pathological nuclear aggregation.
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AN EXAMINATION OF BINDING DIFFERENCES AMONG MISMATCH REPAIR RECOGNITION PROTEINS WITH MISMATCH AND CISPLATIN DAMAGED DNA
… and MSH2-MSH3 were expressed to perform fluorescence anisotropy binding experiments and to crystallize the proteins in complex with cisplatin damaged DNA for comparison to mismatch DNA binding. All three mismatch repair (MMR) recognition complexes proved capable of binding cisplatin …
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Optical properties and biological applications of fluorescent gold nanoclusters
… and intensive interests, the study of the fluorescence mechanism of AuNCs has so far been limited. In order to investigate the factors that determine the fluorescence properties of Bovine Serum Albumin (BSA) encapsulated AuNCs (BSA-AuNCs), the influence of pH during nanocluster growth on …
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Synthesis and characterization of terpyridine-based fluorescent coordination polymers
… complexes have been investigated by UV-vis and fluorescence spectroscopy. The Zn2+ coordination to the terpyridine unit does not effect the advantageous fluorescence properties of perylene bisimide moieties. The reversibility of the formation of coordination polymers has been established by 1H …
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Characterizing interactions between cAMP responsive element binding protein 1 and methyl-CpG-binding protein 2 as a potential transcriptional activation complex
… effects of MeCP2 on CREB1 binding to DNA through fluorescence anisotropy assays. These studies suggest that there is a potential interaction between MeCP2 and CREB1. In addition to examining the presence of interactions between MeCP2 and CREB1, we also make progress on establishing protocols for …
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Application of fluorescence spectroscopy for the in-vial investigation of protein solutions
… of traditional techniques, the development of a fluorescence-based spectroscopic technique for “in-vial” protein analysis was a key thesis aim. A fluorescence excitation wavelength of 365 nm was found and confirmed to be transmissible through borosilicate glass vial walls. The excitation resulted …
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Structural and mechanistic studies on eukaryotic UDP-galactopyranose mutases
… kinetics, trapping of reaction intermediates, fluorescence anisotropy, and X-ray crystallography. The mechanism first involves reduction of the required flavin by NADPH, followed by UDP-Galp binding and subsequent SN2 attack by the flavin on galactose displacing UDP to form a flavin N5-C1 …
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Rapid diffusion in the brain extracellular space - biophysical constraints and physiological implications
… (area CA1). Next, we adopt time‐resolved fluorescence anisotropy imaging microscopy to establish the previously unknown instantaneous diffusivity of small molecules in the extracellular space. The result indicates that nanometer‐scale diffusivity in the brain extracellular space is 25‐30% …
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Molecular Structure-nonlinear Optical Property Relationships For A Series Of Polymethine And Squaraine Molecules
… upon laser intensity, such as two-photon fluorescence imaging, three-dimensional microfabrication, optical data storage and optical limiting, have motivated researchers to find novel materials exhibiting strong 2PA. Organic materials are promising candidates because their linear and …
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Applied molecular quantum electrodynamics: geometric aspects of the efficiency of photonic interactions
… single unspecified chromophore. Anomalously high fluorescence-anisotropy is explainable with the latter analysis. Off-resonant light is known to modify the absorption behaviour of molecules: This weak-interaction is analysed with an MQED formulation modified by field dressing, modelling advanced …
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Protein folding and diffusion: from in vitro to live cells
… the protein diffusion in living cells with fluorescence loss in photobleaching experiment. The temperature dependence of diffusion of proteins with different sizes, stabilities are compared. We studied the anomalous diffusion and microenvironment fluctuation in the cell by comparing the …
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