Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 44 for “"fluorescence activated cell sorting (FACS)"”.
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Isolation, engineering, and characterization of intracellular antibodies specific for the huntingtin protein
… conformation which is prone to aggregation. Intracellular antibodies (intrabodies) against htt have been shown to reduce htt aggregation by binding to the htt fragment and inactivating it or preventing its misfolding. Intrabodies may therefore be a useful gene therapy approach to treatment of the …
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Characterization of Dendritic Cells in the Bovine Mammary Gland
… pathogens could potentially target dendritic cells (DC). While there has been some research describing bovine DC populations in-vitro, little is known about DC in mammary tissue. In this study, immunohistofluorescence was used to identify and localize bovine mammary DC. DC were found in …
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Cell Fate Specification and the Regulation of RNA-dependent DNA Methylation in the Arabidopsis Root Meristem
… a complex tissue capable of generating all the cell types that ultimately make up the root. The work presented in this thesis takes advantage of the versatility of high-throughput sequencing to address two independent questions about the root meristem. Although a lot of information is known …
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Forward and inverse metabolic engineering strategies for improving polyhydroxybyrate production
… engineering (FME) is a rational approach to cellular engineering, relying on an understanding of the entire metabolic network to direct perturbations for phenotype improvement. Conversely, inverse metabolic engineering (IME) uses a global, combinatorial approach to identify genetic loci that …
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Genomewide Crispr/Cas9 Screen Identifies Network of Protein Complexes That Regulate Trim24
… a genomewide CRISPR/Cas9 screen library using fluorescence activated cell sorting (FACS) to identify regulators of TRIM24. The screen was enabled by two innovations. I engineered cells with an in-frame knock-in of mClover3 to the endogenous copy of TRIM24 to allow fluorescent monitoring of …
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„Ex vivo” Replikation des pathogenen Prion Proteins
… konnte durch den Western Blot (Wb) und eine fluorescence activated cell sorting (FACS) Analyse gezeigt werden, sowie die Anreicherung des PrP durch die Circulardichroismus (CD) - Spektroskopie. Das so reguliert exprimierte PrPC wurde mit PrPSc infiziert. Die anschließende …
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Alendronate Alters Single Cell Gene Expression of Cortical Osteoblast Lineage Cells During Bone Loss
… it difficult to examine specific populations of cells which are integral to the tissue using traditional molecular methods. For this study we examined single cell cortical osteoblasts derived from the femurs of C57BL/6J mice. After isolating single cells from bone, we were able to individually …
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Advanced image processing methods for 3D high-throughput microscopy
… algorithm for large-scale 3D imaging of cells and tissues. It automates: (1) selecting in-focus images, (2) detecting features from labelled cells to diffraction-limited puncta, and (3) analysing spatial correlations between cells and proteins. Current tools lack the speed, precision and …
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Elucidating Astrocytic Diversity in Human Cerebral Organoids
… slice cultures derived from human embryonic stem cells (hESCs) were generated. The potential for their long-term growth permits the generation of large numbers of glial cells and allowed for a longitudinal assay of astrocytic diversity. This thesis reports the study of newfound astrocytic …
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Inexpensive technologies enabling widespread utilization of image-predicated cell sorting
The most mature, widespread sorting technology, fluorescence-activated cell sorting (FACS), offers high throughput and sorts predicated on a wide range of phenotypes that can be conveyed through average cellular fluorescence or light scattering levels. However, FACS cannot detect a wide range of …
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Rational design and directed evolution of probe ligases for site-specific protein labeling and live-cell imaging
… However, in order to use these probes for live-cell protein imaging, highly specific labeling methods are required. Here, we will describe three efforts to re-engineer the E. coli enzyme, lipoic acid ligase (LplA), to catalyze the ligation of small-molecule probes onto recombinant proteins. We …
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The Omental Fat Band as an Immunomodulatory Microenvironment for Ovarian Cancer
… concerned with the mechanisms by which malignant cells circumvent cell death signaling and maintain unchecked proliferation, focus has shifted to the complex interactions between the tumor cell and the surrounding microenvironment. Ovarian cancer has one of the highest incidence-to-death ratios of …
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Post-transcriptional regulation of vibrio cholerae virulence activator toxt
… <p>In the last chapter, a method of utilizing fluorescence-activated cell sorting (FACS) technology in conjuction with transposon mutagenesis is described. This method was used to isolate bacterial mutants that produce different gene expression profiles in response to environmental cues while …
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Development of a stem cell-based therapy for the recovery of skeletal muscle mass following immobilization
… effective rehabilitation strategies. Satellite cells are myogenic progenitor cells that render muscle capable of repair and regeneration, yet the role for these cells in fiber growth and restoration of function following disuse is equivocal. Perivascular stem/stromal cells, including pericytes, …
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Involvement of CD133+ stem cells in the chemoresistance of glioma
… mass, there is a subpopulation of cancer stem cells that possess the capacity for selfrenewal, infinite proliferative potential and multipotency. The novel cell-membrane protein CD133 has been identified as a marker of stem-like cells in glioma. Interestingly, the CDI 33+ population of GBM has …
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Toward an improved understanding of the Southern Ocean's biological pump: phytoplankton group-specific contributions to nitrogen and carbon cycling across the Subantarctic Indian Ocean
… is rendered more accurate by coupling either fluorescence-activated cell sorting (FACS; of nano- and picophytoplankton; 0.4-20 μm) or microscopy (for microphytoplankton; >20 um) with groupspecific δ15N measurements. Samples were collected for the analysis of nutrients and nitrate-, FACS-, and …
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Translocations of Actin to Nucleus and p21WAF1 to Cytoplasm after Treatment of HDF Cells with H2O2
… 반복적으로 처리하였다. 세포주기는 propidium iodide로 DNA를 염색하여 fluorescence activated cell sorting (FACS)을 통해서 측정하였으며, reactive oxygen species 생성은 2',7'- dichlorofluorescin diacetate로 염색하여 FACS를 통해서 측정하였다. 단백질의 발현과 세포 내 위치변화는 immunoblotting과 면역형광염색을 수행하여 측정하였다. 세포노화가 진행된 정도는 senescence …
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Characterization of Influenza:Streptococcus pneumoniae synergistic disease and potential for disease alleviation via sphingolipid therapy
… (S1P) signaling, as well as control of intracellular S1P levels via sphingosine kinases (SK). Sphingolipids are involved in a multitude of cellular processes, and are tightly regulated by their metabolizing enzymes. We hypothesize that manipulation of sphingolipid signaling and alteration of …
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Control of Neuroendocrine Cell Physiology by a Single Transcription Factor, Drosophila Basic Helix Loop Helix Regulator DIMMED
<p>Neuroendocrine cells feature a large capacity for the processing, accumulation and regulated release of bioactive peptides and peptide hormones. The ultrastructural correlate of this regulated secretory pathway is a specialized organelle, called a dense core vesicle: DCV). DCVs are typically …
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Aggregation of IgG mAb Biotherapeutics: Sources, Methods of Characterization, and Biological Implications
… assay using human peripheral blood mononuclear cells (PBMC) was investigated. Stir-induced IgG2 mAb aggregates were size-enriched using fluorescence activated cell sorting (FACS) and tested for their ability to induce PBMC cytokine responses, at two phases of the immune response. The …
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